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Abstract

Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8 % (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9 % (55/251) of samples and mixed infections in 37.4 % (94/251), with RVA/HAdV-F being the most frequent association (21.5 %; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.

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2020-10-12
2024-03-29
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References

  1. Liu L, Johnson HL, Cousens S, Perin J, Scott S et al. Child health epidemiology reference group of who and UNICEF. global, regional, and national causes of child mortality: an update systematic analysis for 2010 with time trends since 2000. Lancet 2012; 379:2151–2161
    [Google Scholar]
  2. Institute for Health Metrics and Evaluation (IHME) Findings from the global burden of disease study 2017. The Lancet http://healthdata.org 08/2020
    [Google Scholar]
  3. Krishnan T. Novel human astroviruses: challenges for developing countries. Virusdisease 2014; 25:208–214 [View Article][PubMed]
    [Google Scholar]
  4. Sidoti F, Rittà M, Costa C, Cavallo R. Diagnosis of viral gastroenteritis: limits and potential of currently available procedures. J Infect Dev Ctries 2015; 9:551–561 [View Article][PubMed]
    [Google Scholar]
  5. Lima AAM, Oliveira DB, Quetz JS, Havt A, Prata MMG et al. Etiology and severity of diarrheal diseases in infants at the semiarid region of Brazil: a case-control study. PLoS Negl Trop Dis 2019; 13:e0007154 [View Article][PubMed]
    [Google Scholar]
  6. de Crom SCM, Rossen JWA, van Furth AM, Obihara CC. Enterovirus and parechovirus infection in children: a brief overview. Eur J Pediatr 2016; 175:1023–1029 [View Article][PubMed]
    [Google Scholar]
  7. Amaral MS, Estevam GK, Penatti M, Lafontaine R, Lima IC et al. The prevalence of norovirus, astrovirus and adenovirus infections among hospitalised children with acute gastroenteritis in Porto Velho, state of Rondônia, Western Brazilian Amazon. Mem Inst Oswaldo Cruz 2015; 110:215–221
    [Google Scholar]
  8. do Carmo GMI, Yen C, Cortes J, Siqueira AA, de Oliveira WK et al. Decline in diarrhea mortality and admissions after routine childhood rotavirus immunization in Brazil: a time-series analysis. PLoS Med 2011; 8:e1001024
    [Google Scholar]
  9. Barrella KM, Garrafa P, Monezi TA, Hársi CM, Salvi C et al. Longitudinal study on occurrence of adenoviruses and hepatitis A virus in raw domestic sewage in the city of Limeira, SÃo Paulo. Braz J Microbiol 2009; 40:102–107
    [Google Scholar]
  10. Koo HL, Neill FH, Estes MK, Munoz FM, Cameron A et al. Noroviruses: the most common pediatric viral enteric pathogen at a large university hospital after introduction of rotavirus vaccination. J Pediatric Infect Dis Soc 2013; 2:57–60
    [Google Scholar]
  11. Ramani S, Kang G. Viruses causing childhood diarrhoea in the developing world. Curr Opin Infect Dis 2009; 22:477–482
    [Google Scholar]
  12. Ferreira MSR, Xavier MdaPTP, Tinga ACDC, Rose TL, Fumian TM et al. Assessment of gastroenteric viruses frequency in a children's day care center in Rio de Janeiro, Brazil: a fifteen year study (1994-2008). PLoS One 2012; 7:e33754 [View Article][PubMed]
    [Google Scholar]
  13. Davison AJ, Benkő M, Harrach B. Genetic content and evolution of adenoviruses. J Gen Virol 2003; 84:2895–2908
    [Google Scholar]
  14. Borkenhagen LK, Fieldhouse JK, Seto D, Gray GC. Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect 2019; 8:1679–1687
    [Google Scholar]
  15. ICTV International Committee on Taxonomy of Viruses. https://talk.ictvonline.org/taxonomy/ 08/2020
  16. Lion T. Adenovirus infections in immunocompetent and immunocompromised patients. Clin Microbiol Rev 2014; 27:441–462
    [Google Scholar]
  17. Lynch JP, Fishbein M, Echavarria M. Adenovirus. Semin Respir Crit Care Med 2011; 32:494–511
    [Google Scholar]
  18. Morris CA, Flewett TH, Bryden AS, Davies H. Epidemic viral enteritis in a long-stay children’s ward. Lancet 1975; 1:4–5
    [Google Scholar]
  19. Giordano MO, Ferreyra LJ, Isa MB, Martinez LC, Yudowsky SI et al. The epidemiology of acute viral gastroenteritis in hospitalized children in Cordoba City, Argentina: an insight of disease burden. Rev Inst Med Trop Sao Paulo 2001; 43:193–197
    [Google Scholar]
  20. Tran A, Talmud D, Lejeune B, Jovenin N, Renois F et al. Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in northern France. J Clin Microbiol 2010; 48:1943–1946
    [Google Scholar]
  21. Raboni SM, Damasio GA, Ferreira CE, Pereira LA, Nogueira MB et al. Acute gastroenteritis and enteric viruses in hospitalized children in southern Brazil: aetiology, seasonality and clinical outcomes. Mem Inst Oswaldo Cruz 2014; 109:428–435
    [Google Scholar]
  22. Cruz JR, Cáceres P, Cano F, Flores J, Bartlett A et al. Adenovirus types 40 and 41 and rotaviruses associated with diarrhea in children from Guatemala. J Clin Microbiol 1990; 28:1780–1784
    [Google Scholar]
  23. Gelaw A, Pietsch C, Liebert UG. Genetic diversity of human adenovirus and human astrovirus in children with acute gastroenteritis in Northwest Ethiopia. Arch Virol 2019; 164:2985–2993
    [Google Scholar]
  24. Arashkia A, Bahrami F, Farsi M, Nejati B, Jalilvand S et al. Molecular analysis of human adenoviruses in hospitalized children. J Med Virol 2019; Nov;91:1930–1936
    [Google Scholar]
  25. Banerjee A, De P, Manna B, Chawla-Sarkar M. Molecular characterization of enteric adenovirus genotypes 40 and 41 identified in children with acute gastroenteritis in Kolkata, India during 2013-2014. J Med Virol 2017; 89:606–614
    [Google Scholar]
  26. Kim JS, Lee SK, DH K, Hyun J, Kim HS et al. Associations of adenovirus genotypes in Korean acute gastroenteritis patients with respiratory symptoms and intussusception. Biomed Res Int 2017; 2017:1602054
    [Google Scholar]
  27. Moyo SJ, Hanevik K, Blomberg B, Kommedal O, Nordbø SA et al. Prevalence and molecular characterisation of human adenovirus in diarrhoeic children in Tanzania; a case control study. BMC Infect Dis 2014; 14:666
    [Google Scholar]
  28. Li L, Phan TG, Nguyen TA, Kim KS, Seo JK et al. Molecular epidemiology of adenovirus infection among pediatric population with diarrhea in Asia. Microbiol Immunol 2005; 49:121–128
    [Google Scholar]
  29. Primo D, Pacheco GT, Timenetsky MdoCST, Luchs A. Surveillance and molecular characterization of human adenovirus in patients with acute gastroenteritis in the era of rotavirus vaccine, Brazil, 2012-2017. J Clin Virol 2018; 109:35–40 [View Article][PubMed]
    [Google Scholar]
  30. Hou X, Janes L, Brown SVT, Baker K, Sharma P. Health Financing System Assessment: Papua New Guinea Washington, DC: World Bank Group; 2017
    [Google Scholar]
  31. Levy SE, Myers RM. Advancements in next-generation sequencing. Annu Rev Genomics Hum Genet 2016; 17:95–115
    [Google Scholar]
  32. Watanabe ASA, Luchs A, Leal Élcio, Milagres FAdeP, Komninakis SV et al. Complete genome sequences of six human bocavirus strains from patients with acute gastroenteritis in the North region of Brazil. Genome Announc 6: [View Article]
    [Google Scholar]
  33. Cilli A, Luchs A, Leal E et al. Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil. Mem Inst Oswaldo Cruz 2019; 2019:e180574
    [Google Scholar]
  34. Ribeiro GdeO, Luchs A, Milagres FAdeP, Komninakis SV, Gill DE et al. Detection and characterization of enterovirus B73 from a child in Brazil. Viruses 2018; 11:E16 [View Article][PubMed]
    [Google Scholar]
  35. Luchs A, Leal E, Tardy K et al. The rare enterovirus C99 and echovirus 29 strains in Brazil: potential risks associated to silent circulation. Mem Inst Oswaldo Cruz. 2019; 114:e190160
    [Google Scholar]
  36. Rosa UA, Ribeiro GO, Villanova F, Luchs A, Milagres FAdeP et al. First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil. Sci Rep 2019; 9:18599 [View Article][PubMed]
    [Google Scholar]
  37. Leal Élcio, Luchs A, Milagres FAdeP, Komninakis SV, Gill DE et al. Recombinant strains of human parechovirus in rural areas in the North of Brazil. Viruses 2019; 11:488 [View Article][PubMed]
    [Google Scholar]
  38. da Costa AC, Luchs A, Milagres FAdeP, Komninakis SV, Gill DE et al. Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil. Sci Rep 2018a; 8: [View Article]
    [Google Scholar]
  39. da Costa AC, Luchs A, Milagres FAdeP, Komninakis SV, Gill DE et al. Recombination located over 2A-2B junction ribosome frameshifting region of saffold Cardiovirus. Viruses 2018b; 10:520 [View Article][PubMed]
    [Google Scholar]
  40. Luchs A, Leal E, Komninakis SV et al. Wuhan large pig roundworm virus identified in human feces in Brazil. Virus Genes 2018; 54:470–473
    [Google Scholar]
  41. Tahmasebi R, da Costa AC, Tardy K J, Tinker R, de Padua Milagres FA et al. Genomic analyses of potential novel recombinant human adenovirus C in Brazil. Viruses 12:
    [Google Scholar]
  42. da Costa AC, Leal E, Gill D, Milagres FAdeP, Komninakis SV et al. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes 2019; 55:332–338 [View Article][PubMed]
    [Google Scholar]
  43. Charlys da Costa A, Thézé J, Komninakis SCV, Sanz-Duro RL, Felinto MRL et al. Spread of Chikungunya virus East/Central/South African genotype in northeast Brazil. Emerg Infect Dis 2017; 23:1742–1744 [View Article][PubMed]
    [Google Scholar]
  44. Leal E, Luchs A, Milagres FAP et al. Recombinant strains of human parechovirus in rural areas in the North of Brazil. Viruses Published 2019; 6:488
    [Google Scholar]
  45. Price MN, Dehal PS, Arkin AP. FastTree 2-approximately maximum-likelihood trees for large alignments. PLoS One 2010; 5:e9490
    [Google Scholar]
  46. Posada D. jModelTest: phylogenetic model averaging. Mol Biol Evol 2008; 25:1253–1256
    [Google Scholar]
  47. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549
    [Google Scholar]
  48. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 2015; 1:vev003
    [Google Scholar]
  49. Zhang D, Lou X, Yan H, Pan J, Mao H et al. Metagenomic analysis of viral nucleic acid extraction methods in respiratory clinical samples. BMC Genomics 2018; 19:773
    [Google Scholar]
  50. Martínez-Puchol S, Rusiñol M, Fernández-Cassi X, Timoneda N, Itarte M et al. Characterisation of the sewage virome: comparison of NGS tools and occurrence of significant pathogens. Sci Total Environ 2020; 713:136604
    [Google Scholar]
  51. Mohammad HA, Madi NM, Al-Nakib W. Analysis of viral diversity in stool samples from infants and children with acute gastroenteritis in Kuwait using Metagenomics approach. Virol J 2020; 17:10
    [Google Scholar]
  52. Moore NE, Wang J, Hewitt J, Croucher D, Williamson DA et al. Metagenomic analysis of viruses in feces from unsolved outbreaks of gastroenteritis in humans. J Clin Microbiol 2015; 53:15–21
    [Google Scholar]
  53. Duarte MA, Silva JMF, Brito CR, Teixeira DS, Melo FL et al. Faecal Virome analysis of wild animals from Brazil. Viruses 2019; 11:pii: E803
    [Google Scholar]
  54. Kumthip K, Khamrin P, Ushijima H, Maneekarn N. Enteric and non-enteric adenoviruses associated with acute gastroenteritis in pediatric patients in Thailand, 2011 to 2017. PLoS One. 2019; 14:e0220263
    [Google Scholar]
  55. Magwalivha M, Wolfaardt M, Kiulia NM, van Zyl WB, Mwenda JM et al. High prevalence of species D human adenoviruses in fecal specimens from urban Kenyan children with diarrhea. J Med Virol 2010; 82:77–84
    [Google Scholar]
  56. Harb A, Abraham S, Rusdi B, Laird T, O'Dea M et al. Molecular detection and epidemiological features of selected bacterial, viral, and parasitic enteropathogens in stool specimens from children with acute diarrhea in Thi-Qar Governorate, Iraq. Int J Environ Res Public Health 2019; 16:pii: E1573
    [Google Scholar]
  57. Sanaei Dashti A, Ghahremani P, Hashempoor T, Karimi A. Molecular epidemiology of enteric adenovirus gastroenteritis in under-Old children in Iran. Gastroenterol Res Pract 2016; 2016:1–5 [View Article][PubMed]
    [Google Scholar]
  58. Afrad MH, Avzun T, Haque J, Haque W, Hossain ME et al. Detection of enteric- and non-enteric adenoviruses in gastroenteritis patients, Bangladesh, 2012-2015. J Med Virol 2018; 90:677–684
    [Google Scholar]
  59. La Rosa G, Della Libera S, Petricca S, Iaconelli M, Donia D et al. Genetic diversity of human adenovirus in children with acute gastroenteritis, Albania, 2013-2015, Biomed. Res Int 2015; 2015:142912
    [Google Scholar]
  60. Liu L, Qian Y, Zhang Y, Deng J, Jia L et al. Adenoviruses associated with acute diarrhea in children in Beijing, China. PLoS One 2014; 9:e88791
    [Google Scholar]
  61. Mayindou BG, Ngokana A, Sidibé V, Moundélé F, Koukouikila-Koussounda J et al. Molecular epidemiology and surveillance of circulating rotavirus and adenovirus in Congolese children with gastroenteritis. J Med Virol 2016; 88:596–605
    [Google Scholar]
  62. Ozsari T, Bora G, Kaya B, Yakut K. The prevalence of rotavirus and adenovirus in the childhood gastroenteritis, Jundishapur. J Microbiol 2016; 9:e34867
    [Google Scholar]
  63. Mdo T, Kisielius CJJ, Grisi SJ, Escobar AM, Ueda M et al. Rotavirus, adenovirus, astrovirus, calicivirus and small round virus particles in feces of children with and without acute diarrhea, from 1987 to 1988, in the greater São Paulo. Rev Inst Med Trop Sao Paulo 1993; 35:275–280
    [Google Scholar]
  64. Cardoso DDP, Martins RMB, Kitajima EW, Barbosa AJ, Camarota SCT et al. Rotavirus and adenovirus in 0- to 5-year-old children hospitalized with or without gastroenteritis in Goiâna, go, Brazil. Rev Inst Med Trop São Paulo 1992; 34:433–443
    [Google Scholar]
  65. Amaral MSC, Estevam GK, Penatti M, Lafontaine R, Lima ICG et al. The prevalence of norovirus, astrovirus and adenovirus infections among hospitalised children with acute gastroenteritis in Porto Velho, state of Rondônia, Western Brazilian Amazon. Mem Inst Oswaldo Cruz 2015; 110:215–221
    [Google Scholar]
  66. Andreasi MS, Dd C, Fernandes SM, Tozetti IA, Borges AM et al. Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo grande, MS, Brazil. Mem Inst Oswaldo Cruz 2008; 103:741–744
    [Google Scholar]
  67. Duarte RJ, Mendes EN, Penna FJ, Péret-Filho LA, Magalhães PP. Prevalência baixa de adenovirus em crianças CoM diarréia em Belo Horizonte-MG. J Bras Patol Med Lab 2012; 48:259–263
    [Google Scholar]
  68. Reis TA, Assis AS, do Valle DA, Barletta VH, de Carvalho IP et al. The role of human adenoviruses type 41 in acute diarrheal disease in Minas Gerais after rotavirus vaccination, Braz. J Microbiol 2016; 47:243–250
    [Google Scholar]
  69. Costa L, Siqueira JAM, Portal TM, Sousa EC, Júnior ADC et al. Resque, detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil. Rev Soc Bras Med Trop 2017; 50:621–628
    [Google Scholar]
  70. Magalhães GF, Nogueira PA, Grava AF, Penati M, Silva LHPda et al. Rotavirus and adenovirus in Rondônia. Mem Inst Oswaldo Cruz 2007; 102:555–557 [View Article][PubMed]
    [Google Scholar]
  71. Gray JJ, Kohli E, Ruggeri FM, Vennema H, Sánchez-Fauquier A et al. European multicenter evaluation of commercial enzyme immunoassays for detecting norovirus antigen in fecal samples. Clin Vacc Immunol 2007; 14:1349–1355
    [Google Scholar]
  72. Arowolo KO, Ayolabi C I, Lapinski B, Santos JS, Raboni SM. Epidemiology of enteric viruses in children with gastroenteritis in Ogun state, Nigeria. J Med Virol 2019).; 91:1022–1029
    [Google Scholar]
  73. Kotloff KL, Blackwelder WC, Nasrin D, Nataro JP, Farag TH et al. The global enteric multicenter study (GEMs) of diarrheal disease in infants and young children in developing countries: epidemiologic and clinical methods of the case/control study. Clin Infect Dis 2012; 55:S232–S245
    [Google Scholar]
  74. Filho EP, da Costa Faria NR, Fialho AM, de Assis RS, Almeida MM et al. Adenoviruses associated with acute gastroenteritis in hospitalized and community children up to 5 years old in Rio de Janeiro and Salvador, Brazil. J Med Microbiol 2007; 56:313–319
    [Google Scholar]
  75. Kumthip K, Khamrin P, Ushijima H, Maneekarn N. Enteric and non-enteric adenovirus associated with acute gastroenteritis in pediatric patients in Thailand, 2011 to 2017. PLoS One 2019e0220263
    [Google Scholar]
  76. Ouyang Y, Ma H, Jin M, Wang X, Wang J et al. Etiology and epidemiology of viral diarrhea in children under the age of five hospitalized in Tianjin, China. Arch Virol 2012; 157:881–887 [View Article][PubMed]
    [Google Scholar]
  77. Shigemoto N, Hisatsune Y, Toukubo Y, Tanizawa Y, Shimazu Y et al. Detection of gastroenteritis viruses among pediatric patients in Hiroshima Prefecture, Japan, between 2006 and 2013 using multiplex reverse transcription PCR-based assays involving fluorescent dye-labeled primers. J Med Virol 2017; 89:791–800 [View Article][PubMed]
    [Google Scholar]
  78. Akdag A I, Gupta S, Khan N, Upadhayay A, Ray P. Epidemiology and clinical features of rotavirus, adenovirus, and astrovirus infections and coinfections in children with acute gastroenteritis prior to rotavirus vaccine introduction in Meerut, North India. J Med Virol 2019
    [Google Scholar]
  79. Aktas O, Aydin H, Timurkan MO. A molecular study on the prevalence and coinfections of rotavirus, norovirus, astrovirus and adenovirus in children with gastroenteritis. Minerva Pediatr. 2019; 71:431–437
    [Google Scholar]
  80. Tatte VS, Gopalkrishna V. Detection of different enteric viruses in children with diarrheal disease: evidence of the high frequency of mixed infections. Access Microbiology 2019; 1:
    [Google Scholar]
  81. Tran A, Talmud D, Lejeune B, Jovenin N, Renois F et al. Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in northern France. J Clin Microbiol 2010; 48:1943–1946 [View Article][PubMed]
    [Google Scholar]
  82. Román E, Wilhelmi I, Colomina J, Villar J, Cilleruelo ML et al. Acute viral gastroenteritis: proportion and clinical relevance of multiple infections in Spanish children. J Med Microbiol 2003; 52:435–440
    [Google Scholar]
  83. Taylor MB, Marx FE, Grabow WO. Rotavirus, astrovirus and adenovirus associated with an outbreak of gastroenteritis in a South African child care centre. Epidemiol Infect 1997; 119:227–230 [View Article][PubMed]
    [Google Scholar]
  84. Chhabra P, Payne DC, Szilagyi PG, Edwards KM, Staat MA et al. Etiology of viral gastroenteritis in children. J Infect Dis 2013; 208:790–800
    [Google Scholar]
  85. Lennon G, Cashman O, Lane K, Cryan B, O’Shea H. Prevalence and characterization of enteric adenoviruses in the South of Ireland. J Med Virol 2007; 79:1518–1526
    [Google Scholar]
  86. Soares CC, Volotão EM, Albuquerque MC, da Silva FM, de Carvalho TR et al. Prevalence of enteric adenoviruses among children with diarrhea in four Brazilian cities. J Clin Virol 2002; 23:171–177
    [Google Scholar]
  87. Shimizu H, Phan TG, Nishimura S, Okitsu S, Maneekarn N et al. An outbreak of adenovirus serotype 41 infection in infants and children with acute gastroenteritis in Maizuru City, Japan. Infect Genet Evol 2007; 7:279–284 [View Article][PubMed]
    [Google Scholar]
  88. Seto J, Walsh MP, Metzgar D, Seto D. Computational analysis of adenovirus serotype 5 (HAdV-C5) from an HAdV coinfection shows genome stability after 45 years of circulation. Virology 2010; 404:180–186
    [Google Scholar]
  89. Mahadevan P, Seto J, Tibbetts C, Seto D. Natural variants of human adenovirus type 3 provide evidence for relative genome stability across time and geographic space. Virology 2010; 397:113–118
    [Google Scholar]
  90. Ismail AM, Cui T, Dommaraju K, Singh G, Dehghan S et al. Genomic analysis of a large set of currently-and historically-important human adenovirus pathogens. Emerg Microbes Infect 2018; 7:10
    [Google Scholar]
  91. Li P, Yang L, Guo J, Zou W, Xu X et al. Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai. Sci Rep 2017; 7:3548
    [Google Scholar]
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