1887

Abstract

Members of the genus , including (RLBV), are enveloped plant viruses with segmented genomes of negative-strand RNA, although the complete genome complement for any of these viruses is not yet clear. Currently, wheat mosaic virus has the largest emaravirus genome comprising eight RNAs. Previously, we identified five genomic RNAs for RLBV; here, we identify a further three RNAs (RNA6–8). RNA6–8 encode proteins that have clear homologies to one another, but not to any other emaravirus proteins. The proteins self-interacted in yeast two-hybrid and bimolecular fluorescence complementation (BiFC) experiments, and the P8 protein interacted with the virus nucleocapsid protein (P3) using BiFC. Expression of two of the proteins (P6 and P7) using potato virus X led to an increase in virus titre and symptom severity, suggesting that these proteins may play a role in RLBV pathogenicity; however, using two different tests, RNA silencing suppression activity was not detected for any of the RLBV proteins encoded by RNA2–8.

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2015-11-01
2024-03-29
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References

  1. Bi Y., Tugume A.K., Valkonen J.P.T. 2012; Small-RNA deep sequencing reveals Arctium tomentosum as a natural host of Alstroemeria virus X and a new putative Emaravirus. PLoS One 7:e42758 [View Article][PubMed]
    [Google Scholar]
  2. Ebrahim-Nesbat F., Izadpanah K. 1992; Virus-like particles associated with ringfleck mosaic of mountain ash and a mosaic disease of raspberry in the Bavarian Forest. Eur J Forest Pathol 22:1–10 [View Article]
    [Google Scholar]
  3. Elbeaino T., Digiaro M., Martelli G.P. 2009; Complete nucleotide sequence of four RNA segments of fig mosaic virus. Arch Virol 154:1719–1727 [View Article][PubMed]
    [Google Scholar]
  4. Elbeaino T., Digiaro M., Uppala M., Sudini H. 2014; Deep sequencing of pigeonpea sterility mosaic virus discloses five RNA segments related to emaraviruses. Virus Res 188:27–31 [View Article][PubMed]
    [Google Scholar]
  5. Elbeaino T., Digiaro M., Uppala M., Sudini H. 2015; Deep sequencing of dsRNAs recovered from mosaic-diseased pigeonpea reveals the presence of a novel emaravirus: pigeonpea sterility mosaic virus 2. Arch Virol 160:2019–2029 [CrossRef]
    [Google Scholar]
  6. Garcia S., Billecocq A., Crance J.M., Prins M., Garin D., Bouloy M. 2006; Viral suppressors of RNA interference impair RNA silencing induced by a Semliki Forest virus replicon in tick cells. J Gen Virol 87:1985–1989 [View Article][PubMed]
    [Google Scholar]
  7. Ishikawa K., Maejima K., Komatsu K., Kitazawa Y., Hashimoto M., Takata D., Yamaji Y., Namba S. 2012; Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6. J Gen Virol 93:1612–1619 [View Article][PubMed]
    [Google Scholar]
  8. Ishikawa K., Maejima K., Komatsu K., Netsu O., Keima T., Shiraishi T., Okano Y., Hashimoto M., Yamaji Y., Namba S. 2013; Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement. J Gen Virol 94:682–686 [View Article][PubMed]
    [Google Scholar]
  9. Lacorte C., Ribeiro S.G., Lohuis D., Goldbach R., Prins M. 2010; Potatovirus X and Tobacco mosaic virus-based vectors compatible with the Gateway cloning system. J Virol Methods 164:7–13 [View Article][PubMed]
    [Google Scholar]
  10. Laney A.G., Keller K.E., Martin R.R., Tzanetakis I.E. 2011; A discovery 70 years in the making: characterization of the Rose rosette virus. J Gen Virol 92:1727–1732 [View Article][PubMed]
    [Google Scholar]
  11. Li H., Handsaker B., Wysoker A., Fennell T., Ruan J., Homer N., Marth G., Abecasis G., Durbin R., 1000 Genome Project Data Processing Subgroup. 2009; The Sequence Alignment/Map format and SAMtools. Bioinformatics 25:2078–2079 [View Article][PubMed]
    [Google Scholar]
  12. McGavin W.J., Mitchell C., Cock P.J.A., Wright K.M., MacFarlane S.A. 2012; Raspberry leaf blotch virus, a putative new member of the genus Emaravirus, encodes a novel genomic RNA. J Gen Virol 93:430–437 [View Article][PubMed]
    [Google Scholar]
  13. Mielke N., Muehlbach H.P. 2007; A novel, multipartite, negative-strand RNA virus is associated with the ringspot disease of European mountain ash (Sorbus aucuparia L.). J Gen Virol 88:1337–1346 [View Article][PubMed]
    [Google Scholar]
  14. Mielke-Ehret N., Mühlbach H.P. 2012; Emaravirus: a novel genus of multipartite, negative strand RNA plant viruses. Viruses 4:1515–1536 [View Article][PubMed]
    [Google Scholar]
  15. Mielke-Ehret N., Thoma J., Schlatermund N., Mühlbach H.P. 2010; Detection of European mountain ash ringspot-associated virus-specific RNA and protein P3 in the pear leaf blister mite Phytoptus pyri (Eriophyidae). Arch Virol 155:987–991 [View Article][PubMed]
    [Google Scholar]
  16. Milne I., Stephen G., Bayer M., Cock P.J.A., Pritchard L., Cardle L., Shaw P.D., Marshall D. 2013; Using Tablet for visual exploration of second-generation sequencing data. Brief Bioinform 14:193–202 [View Article][PubMed]
    [Google Scholar]
  17. Sabanadzovic S., Abou Ghanem-Sabanadzovic N. 2011; Emaravirus and cryptovirus infection of Viburnum lantanoides in the Great Smoky Mountains. Phytopathology 101:S158
    [Google Scholar]
  18. Schindelin J., Arganda-Carreras I., Frise E., Kaynig V., Longair M., Pietzsch T., Preibisch S., Rueden C., Saalfeld S., other authors. 2012; Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676–682 [View Article][PubMed]
    [Google Scholar]
  19. Schnettler E., Hemmes H., Huismann R., Goldbach R., Prins M., Kormelink R. 2010; Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules. J Virol 84:11542–11554 [View Article][PubMed]
    [Google Scholar]
  20. Takeda A., Sugiyama K., Nagano H., Mori M., Kaido M., Mise K., Tsuda S., Okuno T. 2002; Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus . FEBS Lett 532:75–79 [View Article][PubMed]
    [Google Scholar]
  21. Tatineni S., McMechan A.J., Wosula E.N., Wegulo S.N., Graybosch R.A., French R., Hein G.L. 2014; An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein. J Virol 88:11834–11845 [View Article][PubMed]
    [Google Scholar]
  22. Uhrig J.F., Mutondo M., Zimmermann I., Deeks M.J., Machesky L.M., Thomas P., Uhrig S., Rambke C., Hussey P.J., Hülskamp M. 2007; The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis. Development 134:967–977 [View Article][PubMed]
    [Google Scholar]
  23. van Rij R.P., Saleh M.C., Berry B., Foo C., Houk A., Antoniewski C., Andino R., The R.N.A. 2006; silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster . Genes Dev 20:2985–2995 [View Article][PubMed]
    [Google Scholar]
  24. Wijkamp I., van Lent J., Kormelink R., Goldbach R., Peters D. 1993; Multiplication of tomato spotted wilt virus in its insect vector. Frankliniella occidentalis. J Gen Virol 74:341–349 [View Article][PubMed]
    [Google Scholar]
  25. Yu C., Karlin D.G., Lu Y., Wright K., Chen J., MacFarlane S. 2013; Experimental and bioinformatic evidence that raspberry leaf blotch emaravirus P4 is a movement protein of the 30K superfamily. J Gen Virol 94:2117–2128 [CrossRef]
    [Google Scholar]
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