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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov. and sp. nov., two novel actinobacteria isolated from rhizosphere soil of Griff. No Access

Abstract

The taxonomic position of two novel strains isolated from rhizosphere soil of wild rice ( Griff.) was established using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains WRP6H-15 and WRP9H-5 were closely related to JCM 3146 and TTN02-30. Chemotaxonomic and morphological characteristics of both strains were consistent with members of the genus , while phenotypic properties, genome-based comparisons and phylogenomic analyses distinguished strains WRP6H-15 and WRP9H-5 from their closest phylogenetic relatives. The two strains showed nearly identical 16S rRNA gene sequences (99.9 %). Strain WRP6H-15 showed 68.7 % digital DNA–DNA hybridization, 95.9 % average nucleotide identity (ANI) based on and 96.4 % ANI based on MUMmer to strain WRP9H-5. A phylogenomic tree based on draft genome sequences of the strains and representative of the genus confirmed the phylogenetic relationships. The genomes sizes of strains WRP6H-15 and WRP9H-5 were 9.42 Mb and 9.68 Mb, with DNA G+C contents of 71.5 and 71.3 mol%, respectively. analysis predicted that the strains contain biosynthetic gene clusters encoding for specialized metabolites. Characterization based on chemotaxonomic, phylogenetic, phenotypic and genomic evidence demonstrated that strains WRP6H-15 and WRP9H-5 represent two novel species of the genus , for which the names sp. nov. (type strain WRP6H-15=TBRC 15726=NBRC 115556) and sp. nov. (type strain WRP9H-5=TBRC 15727=NBRC 115557) are proposed.

  • Received:
  • Accepted:
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Keyword(s): Actinoallomurus , actinomycete , polyphasic taxonomy and wild rice
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006177
2023-11-23
2024-04-28
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Actinoallomurus soli sp. nov. and Actinoallomurus rhizosphaericola sp. nov., two novel actinobacteria isolated from rhizosphere soil of Oryza rufipogon Griff.
Int J Syst Evol Microbiol 73, 006177 (2023); https://doi.org/10.1099/ijsem.0.006177
/content/journal/ijsem/10.1099/ijsem.0.006177
/content/journal/ijsem/10.1099/ijsem.0.006177
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