1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 2

sp. nov., isolated from a sea anemone in the Western Pacific Ocean No Access

Abstract

A novel marine bacterium designated strain PHK-P5 was isolated from a sea anemone ( sp. 1). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PHK-P5 belonged to the genus . The bacterium was Gram-stain-negative, aerobic, oxidase- and catalase- positive, oval- to rod-shaped, and motile. Growth was observed at pH 6.0–9.0, salinities of 2.0–9.0 % and temperatures of 4–37 °C. The G+C content of the chromosomal DNA was 49.2 %. The respiratory quinone was determined to be Q-10. The principal fatty acids of strain PHK-P5 were Ccyclo 8 (25.19 %), C (22.76 %), summed feature 8 (C 7/6; 16.14 %), C (8.81 %), Ccyclo (8.10 %), summed feature 2 (C aldehyde and/or unknown 10.928; 7.19 %) and C 7 11-methyl (5.03 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity and digital DNA–DNA hybridization values among the genomes of strain PHK-P5 and the reference strains were 68.7–70.9 % and 17.4–18.1 %, respectively. The combined genotypic and phenotypic data showed that strain PHK-P5 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain PHK-P5 (=MCCC M21824=KCTC 82924).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): nitrification , seawater , Sneathiella marina sp. nov. and taxonomy
Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2019-9)
    • Principle Award Recipient: ZongzeShao
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award No. 2019021)
    • Principle Award Recipient: ZongzeShao
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: QiliangLai
  • the National Natural Science Foundation of China (Award No.32200045)
    • Principle Award Recipient: GuizhenLi
  • Deep Sea Habitats Discovery Project (Award DY-XZ-04)
    • Principle Award Recipient: ZongzeShao
© 2023 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005721
2023-02-16
2024-05-14
Loading full text...

Full text loading...

References

  1. Jordan EM, Thompson FL, Zhang X-H, Li Y, Vancanneyt M et al. Sneathiella chinensis gen. nov., sp. nov., a novel marine Alphaproteobacterium isolated from coastal sediment in Qingdao, China. Int J Syst Evol Microbiol 2007; 57:114–121 [View Article]
     [Google Scholar]
  2. Kurahashi M, Fukunaga Y, Harayama S, Yokota A. Sneathiella glossodoripedis sp. nov., a marine Alphaproteobacterium isolated from the nudibranch Glossodoris cincta, and proposal of Sneathiellales ord. nov. and Sneathiellaceae fam. nov. Int J Syst Evol Microbiol 2008; 58:548–552 [View Article]
     [Google Scholar]
  3. Siamphan C, Kim H, Lee J-S, Kim W. Sneathiella chungangensis sp. nov., isolated from a marine sand, and emended description of the genus Sneathiella. Int J Syst Evol Microbiol 2014; 64:1468–1472 [View Article] [PubMed]
     [Google Scholar]
  4. Lee SD. Sneathiella limimaris sp. nov., a marine Alphaproteobacterium isolated from a tidal mudflat and emended description of the genus Sneathiella. Int J Syst Evol Microbiol 2019; 69:1993–1997 [View Article] [PubMed]
     [Google Scholar]
  5. Li G, Lai Q, Yan P, Gu L, Shao Z. Sneathiella aquimaris sp. nov., isolated from aquaculture seawater. Int J Syst Evol Microbiol 2020; 70:3824–3831 [View Article] [PubMed]
     [Google Scholar]
  6. Khan SA, Jung HS, Park HY, Jeon CO. Maritimibacter harenae sp. nov. and Sneathiella litorea sp. nov.: members of Alphaproteobacteria isolated from sea sand. Antonie Van Leeuwenhoek 2021; 114:799–811 [View Article] [PubMed]
     [Google Scholar]
  7. Lee Y, Weerawongwiwat V, Kim J-H, Yoon J-H, Suh MK et al. Sneathiella sedimenti sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2022; 72:005218 [View Article] [PubMed]
     [Google Scholar]
  8. Skerman VBD. A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins; 1967
     [Google Scholar]
  9. Dong X-Z, Cai M-Y. Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation); 2001
     [Google Scholar]
  10. Sambrook J, Fritsch EF, Maniatis T. Molecular Coning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1989
     [Google Scholar]
  11. Ausubel F, Brent R, Kingston R, Moore D, Seidman J et al. Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley; 1995
     [Google Scholar]
  12. Liu C, Shao Z. Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 2005; 55:1181–1186 [View Article] [PubMed]
     [Google Scholar]
  13. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article] [PubMed]
     [Google Scholar]
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article] [PubMed]
     [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  17. Rzhetsky A, Nei M. Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 1992; 35:367–375 [View Article] [PubMed]
     [Google Scholar]
  18. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article]
     [Google Scholar]
  19. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  20. Auch AF, Klenk HP, Göker M. Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2010; 2:142–148 [View Article] [PubMed]
     [Google Scholar]
  21. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article] [PubMed]
     [Google Scholar]
  22. Sasser M. Technical Note 101: Identification of bacteria by gas chromatography of cellular fatty acids Newark, DE: MIDI; 1990
     [Google Scholar]
  23. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  24. Cui H-L, Gao X, Yang X, Xu X-W. Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae. Int J Syst Evol Microbiol 2011; 61:1617–1621 [View Article]
     [Google Scholar]
  25. Tindall B. Lipid composition of Halobacterium lacusprofundi. FEMS Microbio Lett 1990; 66:199–202
     [Google Scholar]
  26. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 2009; 106:19126–19131 [View Article]
     [Google Scholar]
  27. Moore WEC, Stackebrandt E, Kandler O, Colwell RR, Krichevsky MI et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005721
Loading
Sneathiella marina sp. nov., isolated from a sea anemone in the Western Pacific Ocean
Int J Syst Evol Microbiol 73, 005721 (2023); https://doi.org/10.1099/ijsem.0.005721
/content/journal/ijsem/10.1099/ijsem.0.005721
/content/journal/ijsem/10.1099/ijsem.0.005721
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error