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Volume 71, Issue 10

Isolation and description of sp. nov., a novel species detected in a gingivitis patient No Access

Abstract

A Gram-stain-negative bacterium, designated strain Marseille-Q3039, was isolated from subgingival dental plaque of a woman with gingivitis in Marseille, France. Strain Marseille-Q3039 was found to be an anaerobic, motile and spore-forming crescent-shaped bacterium that grew at 25–41.5 °C (optimum, 37 °C), pH 5.5–8.5 (optimum, pH 7.5) and salinity of 5.0 g l NaCl. The results of 16S rRNA gene sequence analysis revealed that strain Marseille-Q3039 was closely related to ATCC 43532 (98.42 % similarity), ATCC 43527 (97.25 %) and DSM 2778 (97.19 %). The orthologous average nucleotide identity and digital DNA–DNA hybridization relatedness between strain Q3039 and its closest phylogenetic neighbours were respectively 84.57 and 28.2 % for ATCC 43532 and 83.93 and 27.2 % for DSM 2778. The major fatty acids were identified as C (27.7 %), C (24.4 %) and specific C 3-OH (12.3 %). Genome sequencing revealed a genome size of 2 351 779 bp and a G+C content of 57.2 mol%. On the basis of the results from phenotypic, chemotaxonomic, genomic and phylogenetic analyses and data, we concluded that strain Marseille-Q3039 represents a novel species of the genus , for which the name sp. nov. is proposed (=CSUR Q3039=CECT 30128).

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Keyword(s): culturomics , dental plaque , gingivitis and Selenomonas
Funding
This study was supported by the:
  • Agence Nationale de la Recherche (Award Méditerranée Infection 10-IAHU-03)
    • Principle Award Recipient: NotApplicable
© 2021 The Authors
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2021-10-06
2024-04-19
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Isolation and description of Selenomonas timonae sp. nov., a novel Selenomonas species detected in a gingivitis patient
Int J Syst Evol Microbiol 71, 005040 (2021); https://doi.org/10.1099/ijsem.0.005040
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