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Volume 71, Issue 9

sp. nov., isolated from soil of Pu-erh tea cellar No Access

Abstract

A Gram-reaction-negative, yellow-pigmented, non-spore-forming rod, aerobic, motile bacterium, designated SJY3, was isolated from soil samples collected from a Pu-erh tea cellar in Bolian Pu-erh tea estate Co. Ltd. in Pu'er city, Yunnan, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus . The closest phylogenetic relative was CICC 24458 (99.5 %), followed by CCUG45783 (97.9 %), CCUG43427A (97.8 %), and DSM 18055 (97.8 %). The major fatty acids were C and C 7 and/or C 6. The major respiratory quinone was ubiquinone Q-8 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Genome sequencing revealed a genome size of 5.97 M bp and a G+C content of 65.4 mol%. Pairwise determined whole genome average nucleotide identity (gANI) values and digital DNA–DNA hybridization (dDDH) values were all below the threshold. Although the 16S rRNA gene similarity of stain SJY3 and CICC 24458 was more than 99 %, the gANI, dDDH values and genomic tree clearly indicated that they were not of the same species. In summary, strain SJY3 represents a new species, for which we propose the name sp. nov. with the type strain SJY3 (=CGMCC 1.17158=KCTC 82193).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , Massilia , novel species , polyphasic taxonomy and Pu-erh tea Cellar
Funding
This study was supported by the:
  • Applied Basic Research Key Project of Yunnan (Award 2019ZG00909-02)
    • Principle Award Recipient: LiangYan
© 2021 The Authors
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2021-09-09
2024-04-19
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Massilia puerhi sp. nov., isolated from soil of Pu-erh tea cellar
Int J Syst Evol Microbiol 71, 004992 (2021); https://doi.org/10.1099/ijsem.0.004992
/content/journal/ijsem/10.1099/ijsem.0.004992
/content/journal/ijsem/10.1099/ijsem.0.004992
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