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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 4

sp. nov., isolated from farmland soil Free

Abstract

A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2, was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2 was a member of the genus and most closely related to R-6514 (98.9 % similarity), RA9 (98.0 %) and R-7190 (97.7 %). A draft genome sequence determined for strain 3-2-2 revealed a DNA G+C content of 42.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between 3-2-2 and the closely related species ranged 79.4–84.2 % and 23.4–24.6 %. The major fatty acids of strain 3-2-2 were iso-C, anteiso-C, iso-C and iso-C. The major isoprenoid quinone was MK-7. meso-Diaminopimelic acid was detected in the peptidoglycan. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. The results of phylogenetic analyses, genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 3-2-2 (=CGMCC 1.13685=LMG 30839).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus acidinfaciens and novel species
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41601332 and 41401275)
© 2019 IUMS
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2019-02-12
2024-04-26
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Bacillus acidinfaciens sp. nov., isolated from farmland soil
Int J Syst Evol Microbiol 69, 1075 (2019); https://doi.org/10.1099/ijsem.0.003271
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