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Volume 67, Issue 6

gen. nov., a marine phototrophic member of the family including the description of sp. nov. and the reclassification of Anil Kumar . 2007 as comb. nov. Free

Abstract

A coccoid-shaped phototrophic purple sulfur bacterium, strain AK35, was isolated from a coastal surface water sample collected from Visakhapatnam, India. Cells were Gram-stain-negative, motile and purple, containing bacteriochlorophyll and the carotenoid rhodopinal as major photosynthetic pigments. Strain AK35 was able to grow photoheterotrophically and could utilize a number of organic substrates. It was unable to grow photoautotrophically. Strain AK35 was able to utilize sulfide and thiosulfate as electron donors. The main fatty acids present were identified as C, Cω7, and Cω7 and/or iso-C 2OH (summed feature 3). Strain AK35 contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and six unidentified lipids as polar lipids. The G+C content of the DNA of strain AK35 was 63.1 mol%. 16S rRNA gene sequence comparisons indicated that the isolate represented a member of the family . 16S rRNA gene sequence analysis indicated that strain AK35 is phylogenetically distinctly positioned outside the groups of most members of the genus clustered with members of the genera and , but was most closely related to with a pairwise sequence similarity of 98.75 %. Based on DNA–DNA hybridization between strain AK35 and MTCC 8120 a relatedness of 39.46 % was established. Distinct morphological, physiological and genotypic differences from these previously described taxa supported the classification of the new isolate as a representative of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is AK35 (=JCM 18851=KCTC 15575=MTCC 12304). In addition, is recognized as another species of this genus and transferred to comb. nov.

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  • Accepted:
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Keyword(s): 16S rRNA gene-based phylogeny , chemotaxonomy , Gammaproteobacteria and Imhoffiella purpurea
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2017-06-01
2024-04-19
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References

  1. Anil Kumar P, Srinivas TNR, Sasikala C, Ramana C, Imhoff JF. Allochromatium renukae sp. nov. Int J Syst Evol Microbiol 2008; 58:404–407 [View Article][PubMed]
     [Google Scholar]
  2. Anil Kumar P, Srinivas TNR, Sasikala C, Ramana C, Imhoff JF. Thiophaeococcus mangrovi gen. nov., sp. nov., a photosynthetic, marine gammaproteobacterium isolated from the Bhitarkanika mangrove forest of India. Int J Syst Evol Microbiol 2008; 58:2660–2664 [View Article][PubMed]
     [Google Scholar]
  3. Anil Kumar P, Srinivas TNR, Thiel V, Tank M, Sasikala C et al. Thiohalocapsa marina sp. nov., from an Indian marine aquaculture pond. Int J Syst Evol Microbiol 2009; 59:2333–2338 [View Article][PubMed]
     [Google Scholar]
  4. Imhoff JF. Transfer of Pfennigia purpurea Tindall 1999 (Amoebobacter purpureus Eichler and Pfennig 1988) to the genus Lamprocystis as Lamprocystis purpurea comb. nov. Int J Syst Evol Microbiol 2001; 51:1699–1701 [View Article][PubMed]
     [Google Scholar]
  5. Imhoff JF. Family I. Chromatiaceae Bavendamm 1924, 125AL emend. Imhoff 1984b, 339. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 3–9
     [Google Scholar]
  6. Nupur N, Tanuku NR, Shinichi T, Pinnaka AK. Phaeobacterium nitratireducens gen. nov., sp. nov., a phototrophic gammaproteobacterium isolated from a mangrove forest sediment sample. Int J Syst Evol Microbiol 2015; 65:2357–2364 [View Article][PubMed]
     [Google Scholar]
  7. Srinivas TNR, Anil Kumar P, Sucharitha K, Sasikala C, Ramana C. Allochromatium phaeobacterium sp. nov. Int J Syst Evol Microbiol 2009; 59:750–753 [View Article][PubMed]
     [Google Scholar]
  8. Imhoff JF, Süling J, Petri R. Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 1998; 48:1129–1143 [View Article][PubMed]
     [Google Scholar]
  9. Guyoneaud R, Matheron R, Liesack W, Imhoff JF, Caumette P. Thiorhodococcus minus, gen. nov., sp. nov., A new purple sulfur bacterium isolated from coastal lagoon sediments. Arch Microbiol 1997; 168:16–23 [View Article][PubMed]
     [Google Scholar]
  10. Rabold S, Gorlenko VM, Imhoff JF. Thiorhodococcus mannitoliphagus sp. nov., a purple sulfur bacterium from the White Sea. Int J Syst Evol Microbiol 2006; 56:1945–1951 [View Article][PubMed]
     [Google Scholar]
  11. Anil Kumar P, Sasi Jyothsna TS, Srinivas TNR, Sasikala C, Ramana C et al. et al. Two novel species of marine phototrophic Gammaproteobacteria: Thiorhodococcus bheemlicus sp. nov. and Thiorhodococcus kakinadensis sp. nov. Int J Syst Evol Microbiol 2007; 57:2458–2461 [View Article][PubMed]
     [Google Scholar]
  12. Sucharita K, Sasikala C, Ramana CV. Thiorhodococcus modestalkaliphilus sp. nov. a phototrophic gammaproteobacterium from Chilika salt water lagoon, India. J Gen Appl Microbiol 2010; 56:93–99 [View Article][PubMed]
     [Google Scholar]
  13. Pfennig N, Trüper HG. The family Chromatiaceae. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H et al. (editors) The Prokaryotes. a Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Applications, 2nd ed. Berlin, Heidelberg & New York: Springer; 1992 pp. 3200–3221
     [Google Scholar]
  14. Srinivas TNR, Anil Kumar P, Sasikala C, Ramana C, Süling J et al. Rhodovulum marinum sp. nov., a novel phototrophic purple non-sulfur alphaproteobacterium from marine tides of Visakhapatnam, India. Int J Syst Evol Microbiol 2006; 56:1651–1656 [View Article][PubMed]
     [Google Scholar]
  15. Trüper HG, Pfennig N. Isolation of members of the families Chromatiaceae and Chlorobiaceae. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria Berlin: Springer; 1981 pp. 279–289
     [Google Scholar]
  16. Takaichi S, Shimada K. Characterization of carotenoids in photosynthetic bacteria. Methods Enzymol 1992; 213:374–385 [CrossRef]
     [Google Scholar]
  17. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
     [Google Scholar]
  18. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–206 [CrossRef]
     [Google Scholar]
  19. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
     [Google Scholar]
  20. Sly LI, Blackall LL, Kraat PC, Tian-Shen T, Sangkhobol V. The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 1986; 5:139–156 [CrossRef]
     [Google Scholar]
  21. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  22. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  23. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  24. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526[PubMed]
     [Google Scholar]
  25. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  26. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000 pp. 333
     [Google Scholar]
  27. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  28. Tourova TP, Antonov AS. Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 1987; 19:333–355 [View Article]
     [Google Scholar]
  29. Rakshak K, Ravinder K, Nupur, Srinivas TNR, Anil Kumar P. Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India. Antonie Van Leeuwenhoek 2013; 104:1217–1225 [View Article][PubMed]
     [Google Scholar]
  30. Imhoff JF. Genus II. Allochromatium Imhoff, Süling and Petri 1998b, 1140VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 12–14
     [Google Scholar]
  31. Imhoff JF, Caumette P. Genus III. Halochromatium Imhoff, Süling and Petri 1998b, 1139VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 14–15
     [Google Scholar]
  32. Imhoff JF. Genus IV. Isochromatium Imhoff, Süling and Petri 1998b, 1141VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 15–16
     [Google Scholar]
  33. Imhoff JF. Genus VII. Marichromatium Imhoff, Süling and Petri 1998b, 1140VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 20–21
     [Google Scholar]
  34. Koops HP, Roser AP. Genus VIII. Nitrosococcus Windogradsky 1892, 127AL. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 21–22
     [Google Scholar]
  35. Shivali K, Sasikala C, Ramana C. MLSA barcoding of Marichromatium spp. and reclassification of Marichromatium fluminis (Sucharita et al., 2010) as Phaeochromatium fluminis gen. nov. comb. nov. Syst Appl Microbiol 2012; 35:221–225 [View Article][PubMed]
     [Google Scholar]
  36. Imhoff JF. Genus X. Rhabdochromatium Dilling, Liesack and Pfennig 1996, 362VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 23–24
     [Google Scholar]
  37. Brettar I, Christen R, Höfle MG. Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic sea. Int J Syst Evol Microbiol 2002; 52:1851–1857 [View Article][PubMed]
     [Google Scholar]
  38. Imhoff JF, Madigan MT. Genus XI. Thermochromatium Imhoff, Süling and Petri 1998b, 1140VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 24–25
     [Google Scholar]
  39. Imhoff JF. Genus XII.Thioalkalicoccus Bryantseva, Gorlenko and Imhoff 2000b, 2161VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 25–26
     [Google Scholar]
  40. Rees GN, Harfoot CG, Janssen PH, Schoenborn L, Kuever J et al. Thiobaca trueperi gen. nov., sp. nov., a phototrophic purple sulfur bacterium isolated from freshwater lake sediment. Int J Syst Evol Microbiol 2002; 52:671–678 [View Article][PubMed]
     [Google Scholar]
  41. Imhoff JF, Caumette P. Genus XIII.Thiocapsa Windogradsky 1888, 84AL emend Guyoneaud, Süling, Petri, Matheron, Caumette, Pfennig and Imhoff 1998b, 962. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 26–28
     [Google Scholar]
  42. Imhoff JF. Genus XIV. Thiococcus Imhoff, Süling and Petri 1998b, 1139VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 28–29
     [Google Scholar]
  43. Imhoff JF. Genus XV. Thiocystis Windogradsky 1888, 60AL emend. Imhoff, Süling and Petri 1998b, 1138. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 29–31
     [Google Scholar]
  44. Imhoff JF. Genus XVI. Thiodictyon Windogradsky 1888, 80AL. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 31–32
     [Google Scholar]
  45. Imhoff JF, Pfennig N. Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b. Int J Syst Evol Microbiol 2001; 51:105–110 [View Article][PubMed]
     [Google Scholar]
  46. Imhoff JF, Caumette P. Genus XVIII. Thiohalocapsa Imhoff, Süling and Petri 1998b, 1139VP. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 34–35
     [Google Scholar]
  47. Guyoneaud R, Süling J, Petri R, Matheron R, Caumette P et al. Taxonomic rearrangements of the genera Thiocapsa and Amoebobacter on the basis of 16S rDNA sequence analyses, and description of Thiolamprovum gen. nov. Int J Syst Bacteriol 1998; 48:957–964 [View Article][PubMed]
     [Google Scholar]
  48. Imhoff JF. Genus XX. Thiopedia Windogradsky 1888, 85AL. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 36–37 [CrossRef]
     [Google Scholar]
  49. Overmann J, Fischer U, Pfennig N. A new purple sulfur bacterium from saline littoral sediments, Thiorhodovibrio winogradskyi gen. nov. and sp. nov. Arch Microbiol 1992; 157:329–335 [CrossRef]
     [Google Scholar]
  50. Imhoff JF. Genus XXIII. Thiospirillum Windogradsky 1888, 104AL. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 39–40 [CrossRef]
     [Google Scholar]
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Imhoffiella gen. nov., a marine phototrophic member of the family Chromatiaceae including the description of Imhoffiella purpurea sp. nov. and the reclassification of Thiorhodococcus bheemlicus Anil Kumar et al. 2007 as Imhoffiella bheemlica comb. nov.
Int J Syst Evol Microbiol 67, 1949 (2017); https://doi.org/10.1099/ijsem.0.001891
/content/journal/ijsem/10.1099/ijsem.0.001891
/content/journal/ijsem/10.1099/ijsem.0.001891
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