1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped (1.4–3.6×0.4–0.6 µm) and motile marine bacterium, designated as MEBiC09124, was isolated from tidal flat sediment of Suncheon Bay, South Korea. 16S rRNA gene sequence analysis revealed that strain MEBiC09124 showed high similarity to 3.5X (96.7 %). Growth was observed at 18–38 °C (optimum 30 °C), at pH 4.0–8.5 (optimum pH 7.5) and with 0–6 % (w/v) (optimum 2.5 %) NaCl. The predominant cellular fatty acids were iso-C, iso-C, iso-C and summed feature 9 (comprising 10-methyl C and/or iso-Cω9). The DNA G+C content was 66.1 mol%. The major respiratory quinone was Q-8. Biochemical characteristics such as production of acetoin and enzyme activities of trypsin, -chymotrypsin and -acetyl--glucosaminidase differentiated strain MEBiC09124 from 3.5X. On the basis of data from this polyphasic taxonomic study, strain MEBiC09124 (=KCCM 43131=JCM 30904) is classified as the type strain of a novel species in the genus for which the name sp. nov. is proposed. Emended descriptions of the genus and are also given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001846
2017-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1672.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001846&mimeType=html&fmt=ahah

References

  1. Fang T, Wang H, Huang Y, Zhou H, Dong P. Oleiagrimonas soli gen. nov., sp. nov., a genome-sequenced gammaproteobacterium isolated from an oilfield. Int J Syst Evol Microbiol 2015; 65:1666–1671 [View Article][PubMed]
    [Google Scholar]
  2. Huang Y, Fang T, Wang H, Zhou H. Draft genome sequence of Oleiagrimonas soli 3.5XT, a type species in a newly identified genus, isolated from an oil field in China. Genome Announc 2015; 3:e00469–15 [View Article][PubMed]
    [Google Scholar]
  3. Naushad S, Adeolu M, Wong S, Sohail M, Schellhorn HE et al. A phylogenomic and molecular marker based taxonomic framework for the order Xanthomonadales: proposal to transfer the families Algiphilaceae and Solimonadaceae to the order Nevskiales ord. nov. and to create a new family within the order Xanthomonadales, the family Rhodanobacteraceae fam. nov., containing the genus Rhodanobacter and its closest relatives. Antonie van Leeuwenhoek 2015; 107:467–485 [View Article][PubMed]
    [Google Scholar]
  4. Saddler GS, Bradbury JF. Order III. Xanthomonadales. In Brenner DJ, Kreig NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 63–122 [CrossRef]
    [Google Scholar]
  5. Angelim AL, Costa SP, Farias BC, Aquino LF, Melo VM. An innovative bioremediation strategy using a bacterial consortium entrapped in chitosan beads. J Environ Manage 2013; 127:10–17 [View Article][PubMed]
    [Google Scholar]
  6. Zobell CE. Studies on marine Bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 1941; 4:42–75
    [Google Scholar]
  7. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  8. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  9. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  10. Yang SH, Seo HS, Oh HM, Kim SJ, Lee JH et al. Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale. Int J Syst Evol Microbiol 2013; 63:1105–1110 [View Article][PubMed]
    [Google Scholar]
  11. Collins MD. Isoprenoid quinone analysis in bacterial classification and identification. In Goodfellow M, Minikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp. 267–287
    [Google Scholar]
  12. Stackebrandt E, Liesack W. Nucleic acids and classification. In Goodfellow M, O’Donnell AG. (editors) Handbook of New Bacterial Systematics London: Academic Press; 1993 pp. 158–160
    [Google Scholar]
  13. Giovannoni SJ. The polymerase chain reaction. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: John Wiley and Sons Ltd; 1991 pp. 177–203
    [Google Scholar]
  14. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  15. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  18. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001846
Loading
/content/journal/ijsem/10.1099/ijsem.0.001846
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error