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Volume 67, Issue 2

sp. nov., isolated from the respiratory tract of Free

Abstract

Five strains of Gram-positive-staining, catalase-negative, coccus-shaped, chain-forming organisms isolated separately from the respiratory tracts of five animals in the Qinghai-Tibet Plateau of China were subjected to phenotypic and molecular taxonomic analyses. Comparative analysis of the 16S rRNA gene indicated that these singular organisms represent a new member of the genus , being phylogenetically closest to DSM 101995 (98.4 % similarity). The , and sequence analysis showed interspecies similarity values between HTS2 and DSM 101995, its closest phylogenetic relative based on 16S rRNA gene sequences, of 98.2, 78.8 and 93.7 %, respectively. A whole-genome phylogenetic tree built from 82 core genes of genomes from 16 species of the genus validated that HTS2 forms a distinct subline and exhibits specific phylogenetic affinity with DNA–DNA hybridization of HTS2 showed an estimated DNA reassociation value of 40.5 % with DSM 101995. On the basis of their phenotypic characteristics and phylogenetic findings, it is proposed that the five isolates be classified as representatives of a novel species of the genus , sp. nov. The type strain is HTS2 (=DSM 101997=CGMCC 1.15533). The genome of sp. nov. strain HTS2 contains 2195 genes with a size of 2 275 471 bp and a mean DNA G+C content of 41.3 mol%.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , Marmota himalayana , Qinghai-Tibet Plateau , respiratory tract and Streptococcus himalayensis
© 2017 IUMS
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2017-02-01
2024-04-18
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Streptococcus himalayensis sp. nov., isolated from the respiratory tract of Marmota himalayana
Int J Syst Evol Microbiol 67, 256 (2017); https://doi.org/10.1099/ijsem.0.001609
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