1887

Abstract

The type strain of and 38 strains identified as , four strains of , including the type and two subcultures of the type, two strains of and six unidentified strains that resembled were examined by PCR fingerprints using primers M13 and (GAC)5. The same strains, together with four strains of the recently introduced nom. inval., were sequenced for the D1/D2 domain of the 26S rRNA gene and parts of the ITS domain and also studied for their physiological properties. Of the strains identified previously as , CBS 2076 had the same fingerprint as the type of and strain CBS 4855 was distinct from all other strains. The six strains that resembled were separated into two groups distinct from any of the other clades. A total of six groups obtained by fingerprint and sequence data were evaluated by performing DNA reassociation reactions. Mating experiments among the 35 strains of showed that 15 strains represented one mating type and 16 strains represented the opposite mating type, while four strains were self-sporulating. Teleomorph states were not produced by , or any of the unidentified isolates. However, positive mating reactions were rarely observed in crosses among and some strains of Y. and . Consequently, sharing the same mating type system, and could be considered anamorphs of unnamed species. Results from PCR fingerprints, sequencing and mating studies support the grouping of the studied strains into , , , nom. inval. and three novel species in the clade: sp. nov. (type strain CBS 10146 =NRRL Y-48252; Mycobank number MB 510769), sp. nov. (type strain CBS 10151 =NRRL Y-48253; Mycobank number MB 510770) and sp. nov. (type strain CBS 4855 =NRRL Y-48254; Mycobank number MB 510771).

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2007-10-01
2024-03-19
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References

  1. Abdelal A. T. H., Kennedy E. H., Ahearn D. G. 1977; Purification of a neutral protease from Saccharomycopsis lipolytica . J Bacteriol 130:1125–1129
    [Google Scholar]
  2. Baleiras Couto M. M., Hartog B. J., Huis in't Veld J. H. J., Hofstra H., van der Vossen J. M. B. M. 1996; Identification of spoilage yeasts in a food-production chain by microsatellite polymerase chain reaction fingerprinting. Food Microbiol 13:59–67 [CrossRef]
    [Google Scholar]
  3. Barnett J. A., Payne R. W., Yarrow D. 2000 Yeasts: Characteristics and Identification , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  4. Barns S. M., Lane D. J., Sogin M. L., Bibeau C., Weisburg W. G. 1991; Evolutionary relationships among pathogenic Candida species and relatives. J Bacteriol 173:2250–2255
    [Google Scholar]
  5. Barth G., Gaillardin C. 1996; Yarrowia lipolytica . In Nonconventional Yeasts in Biotechnology: a Handbook pp 313–388 Edited by Wolf K. Berlin & London: Springer;
    [Google Scholar]
  6. Barth G., Gaillardin C. 1997; Physiology and genetics of the dimorphic fungus Yarrowia lipolytica . FEMS Microbiol Rev 19:219–237 [CrossRef]
    [Google Scholar]
  7. Benno Y. 2004; Microbe division (RIKEN BRC JCM). In Annual Report of the Riken Biological Research Center . pp 905–914 Wako, Japan: RIKEN (in Japanese with English translation
  8. Bigey F., Tuery K., Bougard D., Nicaud J., Moulin G. 2003; Identification of a triacylglycerol lipase gene family in Candida deformans : molecular cloning and functional expression. Yeast 20:233–248 [CrossRef]
    [Google Scholar]
  9. de Hoog G. S., Gerrits van den Ende A. H. 1998; Molecular diagnostics of clinical strains of filamentous Basidiomycetes. Mycoses 41:183–189 [CrossRef]
    [Google Scholar]
  10. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [CrossRef]
    [Google Scholar]
  11. Fickers P., Nicaud J. M., Destain J., Thonart P. 2003; Overproduction of lipase by Yarrowia lipolytica mutants. Appl Microbiol Biotechnol 63:136–142 [CrossRef]
    [Google Scholar]
  12. Fournier P., Gaillardin C., Persuy M.-C., Klootwijk J., van Heerikhuizen H. 1986; Heterogeneity in the ribosomal family of the yeast Yarrowia lipolytica : genomic organization and segregation. Gene 42:273–282 [CrossRef]
    [Google Scholar]
  13. Franke-Rinker D., Behrens U., Nockel E., Forner C., Portnowa A. 1983; Joint utilization of glucose and n-alkanes in citric acid synthesis by Saccharomycopsis lipolytica . Z Allg Mikrobiol 23:9–16 [CrossRef]
    [Google Scholar]
  14. Furukawa T., Ogina T., Matsuyoshi T. 1982; Fermentative production of citric acid from n-paraffins by Saccharomycopsis lipolytica . J Ferment Technol 60:281–286
    [Google Scholar]
  15. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [CrossRef]
    [Google Scholar]
  16. Guindon S., Lethiec F., Duroux P., Gascuel O. 2005; phyml Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33:W557–W559 [CrossRef]
    [Google Scholar]
  17. Hadeball W. 1991; Production of lipase by Yarrowia lipolytica . Acta Biotechnol 11:159–167 [CrossRef]
    [Google Scholar]
  18. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  19. Kalle G. P., Gadkari S. V., Deshpande S. Y. 1972; Inducibility of lipase in Candida lipolytica . Indian J Biochem Biophys 9:171–175
    [Google Scholar]
  20. Kapoor K. K., Chaudhary K., Tauro P. 1982; Citric acid. In Prescott & Dunn's Industrial Microbiology , 4th edn. pp 709–747 Edited by Reed G. Westport, CT: AVI Publishing;
    [Google Scholar]
  21. Ko K. S., Jung H. S. 2002; Three nonorthologous ITS1 types are present in a polypore fungus Trichaptum abietinum . Mol Phylogenet Evol 23:112–122 [CrossRef]
    [Google Scholar]
  22. Kurtzman C. P. 2005; New species and a new combination in the Hyphopichia and Yarrowia yeast clades. Antonie van Leeuwenhoek 88:121–130 [CrossRef]
    [Google Scholar]
  23. Kurtzman C. P., Fell J. W. (editors) 1998 The Yeasts: a Taxonomic Study , 4th edn. Amsterdam: Elsevier;
    [Google Scholar]
  24. Kurtzman C. P., Robnett C. J. 1995; Molecular relationships among hyphal ascomycetous yeasts and yeastlike taxa. Can J Bot 73:S824–S830 [CrossRef]
    [Google Scholar]
  25. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [CrossRef]
    [Google Scholar]
  26. Kurtzman C. P., Smiley M. J., Johnson C. J., Wickerham L. J. 1980; Two closely related heterothallic species, Pichia amylophila and Pichia mississipiensis : characterization by hybridization and deoxyribonucleic acid reassociation. Int J Syst Bacteriol 30:206–216 [CrossRef]
    [Google Scholar]
  27. Kurtzman C. P., Boekhout T., Robert V., Fell J. W., Yarrow D., Deak T. 2003; Methods to identify yeasts. In Yeasts in Food pp 69–122 Edited by Boekhout T., Robert V. Hamburg: Behr's Verlag;
    [Google Scholar]
  28. Lachance M.-A., Daniel H. M., Meyer W., Prasad G. S., Gautam S. P., Boundy-Mills K. 2003; The D1/D2 domain of the large-subunit rDNA of the yeast species Clavispora lustianiae is unusually polymorphic. FEMS Yeast Res 4:253–258 [CrossRef]
    [Google Scholar]
  29. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  30. Mattey M. 1992; The production of organic acids. Crit Rev Biotechnol 12:87–132 [CrossRef]
    [Google Scholar]
  31. Meyer W., Latouche G. N., Thanos M., Mitchell T. G., Yarrow D., Schönian G., Sorrell T. C. 1997; Identification of pathogenic yeasts of the imperfect genus Candida by polymerase chain reaction fingerprinting. Electrophoresis 18:1548–1559 [CrossRef]
    [Google Scholar]
  32. Naumov G. I., Naumova E. S., Smith M. Th., de Hoog G. S. 2006; Molecular-genetic diversity of the ascomycetous yeast genus Arthroascus : Arthroascus babjevae sp.nov., Arthroascus fermentans var. arxii var. nov.and geographical populations of Arthroascus schoenii . Int J Syst Evol Microbiol 56:1997–2007 [CrossRef]
    [Google Scholar]
  33. Naumova E., Naumov G., Fournier P., Nguyen H.-V., Gaillardin C. 1993; Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyotyping and hybridization with cloned genes. Curr Genet 23:450–454 [CrossRef]
    [Google Scholar]
  34. Novotný C., Doležalová L., Lieblová J. 1994; Dimorphic growth and lipase production in lipolytic yeasts. Folia Microbiol (Praha 39:71–73 [CrossRef]
    [Google Scholar]
  35. O'Donnell K. 1993; Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics pp 225–233 Wallingford, UK: CAB International;
    [Google Scholar]
  36. O'Donnell K., Cigelnik E. 1997; Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116 [CrossRef]
    [Google Scholar]
  37. Ogrydziak D. M. 1988; Production of alkaline extracellular protease by Yarrowia lipolytica . Crit Rev Biotechnol 8:177–187 [CrossRef]
    [Google Scholar]
  38. Ogrydziak D. M., Scharf S. J. 1982; Alkaline extracellular protease produced by Saccharomycopsis lipolytica CX161-1B. J Gen Microbiol 128:1225–1234
    [Google Scholar]
  39. Oogaki M., Nakahara T., Uchiyama H., Tabuchi T. 1983; Extracellular production of d-(+)-2-hydroxyglutaric acid by Yarrowia lipolytica from glucose under aerobic, thiamine-deficient conditions. Agric Biol Chem 47:2619–2624 [CrossRef]
    [Google Scholar]
  40. Péter G., Dlauchy D., Vasdinyei R., Tornoi-Lehockzi J., Deak T. 2004; Candida galli sp. nov., a new yeast from poultry. Antonie van Leeuwenhoek 86:105–110 [CrossRef]
    [Google Scholar]
  41. Robert V. 2003; Data processing. In Yeasts in Food pp 139–170 Edited by Boekhout T., Robert V. Hamburg: Behr's Verlag;
    [Google Scholar]
  42. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [CrossRef]
    [Google Scholar]
  43. Seidler R. J., Mandel M. 1971; Quantitative aspects of deoxy-ribonucleic acid renaturation: base composition, site of chromosome replication, and polynucleotide homologies. J Bacteriol 106:608–614
    [Google Scholar]
  44. Smith M. T., de Cock A. W. A. M., Poot G. A., Steensma H. Y. 1995; Genome comparisons in the yeastlike fungal genus Galactomyces Redhead et Malloch. Int J Syst Bacteriol 45:826–831 [CrossRef]
    [Google Scholar]
  45. Smith M. Th., Robert V., Poot G. A., Epping W., de Cock A. W. A. M. 2005; Taxonomy and phylogeny of the ascomycetous yeast genus Zygoascus , with proposal of Zygoascus meyerae sp. nov.. and related anamorphic varieties. Int J Syst Evol Microbiol 55:1353–1363 [CrossRef]
    [Google Scholar]
  46. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  47. Tsugawa R., Nakase T., Koyabashi T., Yamashita K., Okumura S. 1969; Fermentation of n-paraffins by yeast. Part III. α -Ketoglutarate productivity of various yeasts. Agric Biol Chem 33:929–938 [CrossRef]
    [Google Scholar]
  48. Ueda-Nishimura K., Mikata K. 2002; Species distinction of the ascomycetous heterothallic yeast-like fungus Stephanoascus ciferrii complex: description of Candida allociferrii sp. nov. and reinstatement of Candida mucifera Kocková-Kratochvílová et Sláviková. Int J Syst Evol Microbiol 52:463–471
    [Google Scholar]
  49. van der Walt J. P., von Arx J. A. 1980; The yeast genus Yarrowia gen. nov. Antonie van Leeuwenhoek 46:517–521 [CrossRef]
    [Google Scholar]
  50. Vassart G., Georges M., Monsieur R., Brocas H., Lequarré A. S., Christophe D. 1987; A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235:683–684 [CrossRef]
    [Google Scholar]
  51. Vilgalys R., Hester M. 1990; Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246
    [Google Scholar]
  52. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications pp 315–322 Edited by Innis N., Gelfand D., Sninsky J., White T. New York: Academic Press;
    [Google Scholar]
  53. Wickerham L. J., Kurtzman C. P., Herman A. I. 1970; Sexuality in Candida lipolytica . In Recent Trends in Yeast Research (Spectrum , vol. 1) pp 31–92 Edited by Ahearn D. G. Atlanta: Georgia State University;
    [Google Scholar]
  54. Yamada T., Ogrydziak D. M. 1983; Extracellular acid proteases produced by Saccharomycopsis lipolytica . J Bacteriol 154:23–31
    [Google Scholar]
  55. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts: a Taxonomic Study , 4th edn. pp 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
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