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Volume 57, Issue 2

sp. nov., isolated from activated sludge Free

Abstract

A Gram-negative bacterium, designated strain EMB117, was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25–30 °C and pH 7.0–8.0. Strain EMB117 contained phosphatidylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (C 7 and/or iso-C 2-OH). The G+C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB117 belonged to the genus and was most closely related to DSM 425 (97.8 % sequence similarity). The DNA–DNA relatedness between strain EMB117 and ATCC 17061 was about 18 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EMB117 (=KCTC 12612=DSM 17963).

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2007-02-01
2024-04-24
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References

  1. Anderson R. L., Ordal E. J. 1961; Cytophaga succinicans sp. n., a facultatively anaerobic, aquatic myxobacterium. J Bacteriol 81:130–138
     [Google Scholar]
  2. Aslam Z., Im W. T., Kim M. K., Lee S. T. 2005; Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751 [CrossRef]
     [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978. Int J Syst Bacteriol 46:128–148 [CrossRef]
     [Google Scholar]
  4. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
     [Google Scholar]
  5. Cole J. R., Chai B., Marsh T. L., Farris R. J., Wang Q., Kulam S. A., Chandra S., McGarrell D. M., Schmidt T. M. other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
     [Google Scholar]
  6. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
     [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  8. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146
     [Google Scholar]
  9. Horn M. A., Ihssen J., Matthies C., Schramm A., Acker G., Drake H. L. 2005; Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa . Int J Syst Evol Microbiol 55:1255–1265 [CrossRef]
     [Google Scholar]
  10. Humphry D. R., George A., Black G. W., Cummings S. P. 2001; Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51:1235–1243
     [Google Scholar]
  11. Jeon C. O., Lim J. M., Lee J. M., Xu L. H., Jiang C. L., Kim C. J. 2005; Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896 [CrossRef]
     [Google Scholar]
  12. Kim B. Y., Weon H. Y., Cousin S., Yoo S. H., Kwon S. W., Go S. J., Stackebrandt E. 2006; Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea. Int J Syst Evol Microbiol 56:1645–1649 [CrossRef]
     [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–208
     [Google Scholar]
  15. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  16. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
     [Google Scholar]
  17. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  18. Lim J. M., Jeon C. O., Park D. J., Kim H. R., Yoon B. J., Kim C. J. 2005; Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus . Int J Syst Evol Microbiol 55:1027–1031 [CrossRef]
     [Google Scholar]
  19. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov. and Flavobacterium xanthum sp. nov., nom.rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. . Int J Syst Evol Microbiol 50:1055–1063 [CrossRef]
     [Google Scholar]
  20. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  21. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R.., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
     [Google Scholar]
  22. Stanier R. Y. 1947; Studies on nonfruiting myxobacteria. I Cytophaga johnsonae , n. sp., a chitin-decomposing myxobacterium. J Bacteriol 53:297–315
     [Google Scholar]
  23. Strohl W. R., Tait L. R. 1978; Cytophaga aquatilis sp. nov., a facultative anaerobe isolated from the gills of freshwater fish. Int J Syst Bacteriol 28:293–303 [CrossRef]
     [Google Scholar]
  24. Tamaki H., Hanada S., Kamagata Y., Nakamura K., Nomura N., Nakano K., Matsumura M. 2003; Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53:519–526 [CrossRef]
     [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  27. Van Trappen S., Mergaert J., Van Eygen S., Dawyndt P., Cnockaert M. C., Swings J. 2002; Diversity of 746 heterotrophic bacteria isolated from microbial mats from ten Antarctic lakes. Syst Appl Microbiol 25:603–610 [CrossRef]
     [Google Scholar]
  28. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov. novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:85–92 [CrossRef]
     [Google Scholar]
  29. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2005; Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55:769–772 [CrossRef]
     [Google Scholar]
  30. Wang Z.-W., Liu Y.-H., Dai X., Wang B.-J., Jiang C.-Y., Liu S.-J. 2006; Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:439–442 [CrossRef]
     [Google Scholar]
  31. Yi H., Chun J. 2006; Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 56:1239–1244 [CrossRef]
     [Google Scholar]
  32. Yoon J.-H., Kang S.-J., Oh T.-K. 2006; Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:997–1000 [CrossRef]
     [Google Scholar]
  33. Zhu F., Wang S., Zhou P. 2003; Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53:853–857 [CrossRef]
     [Google Scholar]
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Flavobacterium defluvii sp. nov., isolated from activated sludge
Int J Syst Evol Microbiol 57, 233 (2007); https://doi.org/10.1099/ijs.0.64669-0
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