1887

Abstract

Two mesophilic, anaerobic bacterial strains (ZLJ115 and L4-2) were isolated from the sludge of an anaerobic digester treating municipal solid waste and sewage in Fujian province, China. The strains were Gram-positive, spore-forming, motile rods (0·9–1·0×3·6–7·3 μm). Growth of the strains was observed at 20–42 °C and pH 6·0–9·5. Both strains fermented several mono- and disaccharides. The main fermentation products from glucose were acetate, ethanol, hydrogen and carbon dioxide. Optimal hydrogen production by the new isolates was observed at pH 8·8 and 39 °C, and 1·4 mol H was detected from fermentation of 1 mol glucose. The DNA G+C contents of strains ZLJ115 and L4-2 were 53·9 and 54·3 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represented a novel phyletic sublineage within cluster XI of the clostridia, clustering with four thermophilic species, with <93·8 % 16S rRNA gene sequence similarity to previously described species. Phenotypically, the new isolates were distinguished from their phylogenetic relatives by growing mesophilically and by fermenting a variety of pentoses, as well as their higher genome DNA G+C content. On the basis of polyphasic evidence from this study, a novel genus and species are proposed, gen. nov., sp. nov.; strain ZLJ115 (=DSM 16796=AS 1.5019) is the type strain of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63686-0
2006-04-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/4/721.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63686-0&mimeType=html&fmt=ahah

References

  1. Alain K., Pignet P., Zbinden M. 8 other authors 2002; Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52:1621–1628 [CrossRef]
    [Google Scholar]
  2. Cato E. P., George W. L., Finegold S. M. 1986; Genus Clostridium Prazmowski 1880, 23AL . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1141–1200 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Chen S., Dong X. 2004; Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill wastewater. Int J Syst Evol Microbiol 54:2257–2262 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1993 phylip – Phylogenetic Inference Package, version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  8. Fendrich C., Hippe H., Gottschalk G. 1990; Clostridium halophilium sp. nov. and C. litorale sp. nov., an obligate halophilic and a marine species degrading betaine in the Stickland reaction. Arch Microbiol 154:127–132 [CrossRef]
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Hungate R. E. 1969; A roll-tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  11. Kumar S., Tamura K., Jakobsen I.-B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  12. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy , Special Publication 6 pp  227–291 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  13. Li Y., Mandelco L., Wiegel J. 1993; Isolation and characterization of a moderately thermophilic anaerobic alkaliphile, Clostridium paradoxum sp. nov. Int J Syst Bacteriol 43:450–460 [CrossRef]
    [Google Scholar]
  14. Li Y., Engle M., Weiss N., Mandelco L., Wiegel J. 1994; Clostridium thermoalcaliphilum sp. nov., an anaerobic and thermotolerant facultative alkaliphile. Int J Syst Bacteriol 44:111–118 [CrossRef]
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  17. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  18. Nandi R., Sengupta S. 1998; Microbial production of hydrogen: an overview. Crit Rev Microbiol 24:61–84 [CrossRef]
    [Google Scholar]
  19. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note 101: Newark, DE: MIDI, Inc;
    [Google Scholar]
  20. Slobodkin A. L., Tourova T. P., Kostrikina N. A., Chernyh N. A., Bonch-Osmolovskaya E. A., Jeanthon C., Jones B. E. 2003; Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 53:1131–1134 [CrossRef]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Woods W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  23. Urios L., Cueff V., Pignet P., Barbier G. 2004; Tepidibacter formicigenes sp. nov., a novel spore-forming bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent. Int J Syst Evol Microbiol 54:439–443 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63686-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63686-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error