1887

Abstract

A yellow-pigmented bacterial strain (CC-H3-2), isolated from the rhizosphere of L. (garden lettuce) in Taiwan, was investigated using a polyphasic taxonomic approach. The cells were Gram-negative, rod-shaped and non-spore-forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus , with the highest sequence similarity to the type strains of (97·7 %), (97·5 %), (97·2 %) and (97·2 %). The major whole-cell fatty acids were iso-C (52·2 %) and iso-C 3-OH. DNA–DNA hybridization experiments revealed levels of only 27·4 % to , 27·1 % to , 14·1 % to and 7·8 % to . DNA–DNA relatedness and biochemical and chemotaxonomic properties demonstrate that strain CC-H3-2 represents a novel species, for which the name sp. nov. is proposed. The type strain is CC-H3-2 (=CCUG 49271=CIP 108367).

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/content/journal/ijsem/10.1099/ijs.0.63331-0
2005-01-01
2024-03-19
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References

  1. Cowan S. T. 1974 Cowan and Steel's Manual for the Identification of Medical Bacteria , 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Edwards U., Rogall T., Blocker H., Emde M., Bottger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853 [CrossRef]
    [Google Scholar]
  3. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  4. Hugo C. J., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
    [Google Scholar]
  5. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  6. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  7. Li Y., Kawamura Y., Fujiwara N., Naka T., Liu H., Huang X., Kobayashi K., Ezaki T. 2003; Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Syst Appl Microbiol 26:523–528 [CrossRef]
    [Google Scholar]
  8. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  9. Venter H. 1987; Purification and characterization of a heat stable metalloprotease from a Chryseobacterium of dairy origin . MSc thesis University of Orange Free State; Bloemfontein: South Africa;
  10. Yamaguchi S., Yokoe M. 2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343 [CrossRef]
    [Google Scholar]
  11. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
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