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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_4

gen. nov., sp. nov., a thermophilic and slightly halophilic filamentous bacterium from the phylum Free

Abstract

A strictly aerobic, thermophilic, moderately acidophilic, non-spore-forming bacterium, strain P373, was isolated from geothermally heated soil at Waikite, New Zealand. Cells were filamentous rods, 0.2–0.4 µm in diameter and grew in chains up to 80 µm in length. On the basis of 16S rRNA gene sequence similarity, strain P373 was shown to belong to the family (class ) of the phylum , with the most closely related cultivated strain, UQM 2034, having 87.6 % sequence similarity. Cells stained Gram-negative, and were catalase- and oxidase-positive. The major fatty acids were i-15 : 0 (10.8 %), i-17 : 0 (24.5 %) and i-17 : 0 3-OH (35.2 %). Primary lipids were phosphatidylethanolamine, two unidentified aminolipids and three other unidentified polar lipids. The presence of sulfonolipids (-acyl-capnines) was observed in the total lipid extract by mass spectrometry. The G+C content of the genomic DNA was 47.3 mol% and the primary respiratory quinone was MK-7. Strain P373 grew at 35–63 °C with an optimum temperature of 60 °C, and at pH 5.5–8.7 with an optimum growth pH of 7.3–7.4. NaCl tolerance was up to 5 % (w/v) with an optimum of 0.1–0.25 % (w/v). Cell colonies were non-translucent and pigmented vivid yellow–orange. Cells displayed an oxidative chemoheterotrophic metabolism. The distinct phylogenetic position and the phenotypic characteristics separate strain P373 from all other members of the phylum and indicate that it represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is P373 ( = ICMP 20041 = DSM 27268).

  • Published Online:
Funding
This study was supported by the:
  • Geothermal Resources of New Zealand (GRN) Programme at GNS Science
  • Wairakei Environmental Mitigation Charitable Trust (WEMCT)
© 2014 Institute of Geological and Nuclear Sciences (GNS Science)
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2014-04-01
2024-04-19
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Thermoflavifilum aggregans gen. nov., sp. nov., a thermophilic and slightly halophilic filamentous bacterium from the phylum Bacteroidetes
Int J Syst Evol Microbiol 64, 1264 (2014); https://doi.org/10.1099/ijs.0.057463-0
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