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Volume 64, Issue Pt_6

gen. nov., sp. nov., isolated from coking wastewater activated sludge, and reclassification of as comb. nov. Free

Abstract

A taxonomic study was carried out on strain GCS-AE-31, which was isolated from a phenol-degrading consortium, enriched from coking wastewater activated sludge of the Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AE-31 were Gram-stain-negative, short rods, motile by gliding, oxidase- and catalase-positive. Growth was observed at salinities of 0–3 % and at temperatures of 10–37 °C. On the basis of 16S rRNA gene sequence similarity, strain GCS-AE-31 was most closely related to LMG 10337 (96.17 %), but it showed low similarity to all other species of the genus (89.28–92.45 %). It also showed low 16S rRNA gene similarity to all other species of the family (87.25–92.45 %) examined. The dominant fatty acids were iso-C, summed feature 3 (Cω7/Cω6), anteiso-C and iso-C 3-OH. The menaquinones were MK-7 (95.5 %) and MK-6 (4.5 %). The polar lipids were phosphatidylethanolamine, three aminolipids and three unknown phospholipids. Sphingolipid was present. The G+C content of the chromosomal DNA was 36.2 mol%. According to its phylogenetic position and phenotypic traits, the novel strain could not be assigned to the genus ; it should be classified as representing a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed (type strain GCS-AE-31 = MCCC 1A01299 = CGMCC 1.12329 = LMG 27180). The misclassified species is transferred to the novel genus as comb. nov. (type strain LMG 10337 = MCCC 1A06472 = DSM 12145 = CCUG 39354 = CIP 105500 = JCM 21818 = NBRC 100064).

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Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2012-9, NIMR-2013-9 and NIMR-2014-9)
© 2014 IUMS
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2014-06-01
2024-04-19
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) ( 1995 ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
     [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  3. Cao J. W., Dong C. M., Cao H. B., Shao Z. Z. ( 2011 ). [Isolation of phenol-degrading bacteria from coking wastewater and their degradation gene]. . Huan Jing Ke Xue 32, 560–566 (in Chinese). [PubMed]
    [Google Scholar]
  4. Essam T., Amin M. A., El Tayeb O., Mattiasson B., Guieysse B. ( 2010 ). Kinetics and metabolic versatility of highly tolerant phenol degrading Alcaligenes strain TW1. . J Hazard Mater 173, 783–788. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  6. Kates M. ( 1986 ). Techniques of lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
     [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  8. Lai Q., Yuan J., Gu L., Shao Z. ( 2009 ). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. . Int J Syst Evol Microbiol 59, 1278–1281. [View Article] [PubMed]
    [Google Scholar]
  9. Liu C., Shao Z. ( 2005 ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55, 1181–1186. [View Article] [PubMed]
    [Google Scholar]
  10. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297–306. [View Article] [PubMed]
    [Google Scholar]
  11. Rzhetsky A., Nei M. ( 1992 ). A simple method for estimating and testing minimum evolution trees. . Mol Biol Evol 9, 945–967.
     [Google Scholar]
  12. Rzhetsky A., Nei M. ( 1993 ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10, 1073–1095.[PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  14. Sambrook J., Fritsch E. F., Maniatis T. ( 1989 ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
     [Google Scholar]
  15. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI;.
     [Google Scholar]
  16. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. ( 1998 ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48, 165–177. [View Article] [PubMed]
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  18. Zimmermann J. J., Langer R., Cooney C. L. ( 1990 ). Specific plate assay for bacterial heparinase. . Appl Environ Microbiol 56, 3593–3594.[PubMed]
    [Google Scholar]
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Pseudopedobacter beijingensis gen. nov., sp. nov., isolated from coking wastewater activated sludge, and reclassification of Pedobacter saltans as Pseudopedobacter saltans comb. nov.
Int J Syst Evol Microbiol 64, 1853 (2014); https://doi.org/10.1099/ijs.0.053991-0
/content/journal/ijsem/10.1099/ijs.0.053991-0
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