1887

Abstract

A novel coryneform bacterium, designated strain GIMN1.010, was isolated from a sand sample collected in the desert in the west of China. Cells were Gram-stain-positive, non-spore-forming, catalase-positive, irregular rods. Comparative 16S rRNA gene sequence analysis demonstrated that strain GIMN1.010 belonged to the genus and was related closely to ATCC 13032 (98.4 % similarity). However, the level of DNA–DNA relatedness between strain GIMN1.010 and ATCC 13032 was only 22.4±1.72 %, showing that strain GIMN1.010 represented a genomic species distinct from On the basis of phenotypic and phylogenetic data, strain GIMN1.010 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GIMN1.010 ( = CCTCC AB 2010341  = NRRL B-59552).

Funding
This study was supported by the:
  • Ministry of Science and Technology of China (Award 2006AA020101, 2006AA02Z229, 2007AA021305 and 2007CB707805)
  • National Natural Science Foundation of China (Award 30670050)
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2012-04-01
2024-04-18
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References

  1. Collins M. D., Cummins C. S. 1986; Genus Corynebacterium Lehmann and Neumann 1896, 350AL . In Bergey’s Manual of Systematic Bacteriology vol. 21266–1276 Edited by Sneath P. H. A. , Mair N. S., Sharpe M. E. , Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Cowan S. T., Barrow G. I., Steel K. J., Feltham R. K. A. 2003 Cowan and Steel’s Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  4. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of inuent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
    [Google Scholar]
  5. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
    [Google Scholar]
  6. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  7. Liebl W. 1992; The genus Corynebacterium – nonmedical. In The Prokaryotes pp. 1157–1171 Edited by Balows A., Trüper H. G. , Dworkin M., Harder W. , Schleifer K.-H. New York: Springer;
    [Google Scholar]
  8. Liebl W., Ehrmann M., Ludwig W., Schleifer K. H. 1991; Transfer of Brevibacterium divaricatum DSM 20297T, “Brevibacterium flavum” DSM 20411, “Brevibacterium lactofermentum” DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns. Int J Syst Bacteriol 41:255–260 [View Article][PubMed]
    [Google Scholar]
  9. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  10. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [View Article]
    [Google Scholar]
  11. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  12. Owen R. J., Lapage S. P. 1976; The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. J Appl Bacteriol 41:335–340 [View Article][PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  15. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  16. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
    [Google Scholar]
  17. Yuan M., Zhang W., Dai S., Wu J., Wang Y., Tao T., Chen M., Lin M. 2009; Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium. Int J Syst Evol Microbiol 59:1513–1517 [View Article][PubMed]
    [Google Scholar]
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