1887

Abstract

Two Gram-negative, aerobic, rod-shaped bacteria, designated strains SL-1 and F11, which had the ability to decompose polycyclic aromatic hydrocarbons (PAHs), were isolated from soil samples contaminated by oil. The cells were motile by polar or lateral flagella. According to comparison of 16S rRNA gene sequences, strains SL-1 and F11 were identical and showed the greatest degree of similarity (96.8 %) to both Alt505 and CCBAU 25010; however, only with SL-1 and F11 formed a separate clade. There were low similarities (<90 %) between the and sequences of the two strains and those of the genus of . The bacteria grew at temperatures of 10–40 °C with an optimum of 30 °C. The pH range for growth was 6.0–10.0 and optimum pH was 7.0–8.0. Growth occurred at NaCl concentrations up to 3.0 % (w/v). They were catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (18 : 1ω7c and/or 18 : 1ω6c) and 16 : 0. The DNA G+C content was 62.2 mol%. Strain SL-1 showed 29 and 0 % DNA–DNA relatedness, respectively, with the most related strains Alt505 and CCBAU 25010 according to phylogenic analysis of the 16S rRNA gene. According to physiological and biochemical characteristics and genotypic data obtained in this work, the bacteria represent a novel species of the genus , and the name is proposed. The type strain is SL-1 ( = ACCC 11238 = KCTC 23288) and it could nodulate in nodulation tests.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30900001)
  • Special Scientific Research Funds for Commonweal Agricultural Industry (Award 200803029)
  • Special Foundation for Central Academy of Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (Award 2011-11)
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2012-08-01
2024-03-19
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References

  1. Berge O., Lodhi A., Brandelet G., Santaella C., Roncato M. A., Christen R., Heulin T., Achouak W. 2009; Rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres. Int J Syst Evol Microbiol 59:367–372 [View Article][PubMed]
    [Google Scholar]
  2. Chaintreuil C., Giraud E., Prin Y., Lorquin J., A., Gillis M., de Lajudie P., Dreyfus B. 2000; Photosynthetic bradyrhizobia are natural endophytes of the African wild rice Oryza breviligulata . Appl Environ Microbiol 66:5437–5447 [View Article][PubMed]
    [Google Scholar]
  3. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:738–754[PubMed]
    [Google Scholar]
  4. Dong X.-Z., Cai M.-Y. (editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp. 370–398 Beijing: Science Press; (in Chinese)
    [Google Scholar]
  5. Gao J. L., Turner S. L., Kan F. L., Wang E. T., Tan Z. Y., Qiu Y. H., Gu J., Terefework Z., Young J. P. W. other authors 2004; Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China. Int J Syst Evol Microbiol 54:2003–2012 [View Article][PubMed]
    [Google Scholar]
  6. Graham P. H., Sadowsky M. J., Keyser H. H., Barnet Y. M., Bradley R. S., Cooper J. E., De Ley D. J., Jarvis B. D. W., Roslycky E. B. other authors 1991; Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int J Syst Bacteriol 41:582–587 [View Article]
    [Google Scholar]
  7. Jordan D. C. 1984; Genus I. Rhizobium Frank 1889, 338AL . In Bergey’s Manual of Systematic Bacteriology vol. 1 pp. 235–242 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Kuykendall L. D., Young J., Martínez-Romero E., Kerr A., Sawada H. 2005; Genus I. Rhizobium . In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 325–340 Edited by Garrity G. M., Brenner D. J., Krieg N. R., Staley J. R. Springer: Verlag; [View Article]
    [Google Scholar]
  9. Lin D. X., Chen W. F., Wang F. Q., Hu D., Wang E. T., Sui X. H., Chen W. X. 2009; Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes. Int J Syst Evol Microbiol 59:1919–1923 [View Article][PubMed]
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  12. Martens M., Delaere M., Coopman R., De Vos P., Gillis M., Willems A. 2007; Multilocus sequence analysis of Ensifer and related taxa. Int J Syst Evol Microbiol 57:489–503 [View Article][PubMed]
    [Google Scholar]
  13. Martens M., Dawyndt P., Coopman R., Gillis M., De Vos P., Willems A. 2008; Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium). Int J Syst Evol Microbiol 58:200–214 [View Article][PubMed]
    [Google Scholar]
  14. Menna P., Pereira A. A., Bangel E. V., Hungria M. 2009; Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool. Symbiosis 48:120–130 [View Article]
    [Google Scholar]
  15. Parshetti G., Saratale G., Telke A., Govindwar S. 2009; Biodegradation of hazardous triphenylmethane dye methyl violet by Rhizobium radiobacter (MTCC 8161). J Basic Microbiol 49:Suppl. 1S36–S42 [View Article][PubMed]
    [Google Scholar]
  16. Peng G. X., Yuan Q. H., Li H. X., Zhang W., Tan Z. Y. 2008; Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta . Int J Syst Evol Microbiol 58:2158–2163 [View Article][PubMed]
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  19. Sullivan J. T., Eardly B. D., van Berkum P., Ronson C. W. 1996; Four unnamed species of nonsymbiotic rhizobia isolated from the rhizosphere of Lotus corniculatus. Appl Environ Microbiol 62:2818–2825[PubMed]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  21. Tan Z., Hurek T., Vinuesa P., Müller P., Ladha J. K., Reinhold-Hurek B. 2001; Specific detection of Bradyrhizobium and Rhizobium strains colonizing rice (Oryza sativa) roots by 16S-23S ribosomal DNA intergenic spacer-targeted PCR. Appl Environ Microbiol 67:3655–3664 [View Article][PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  23. Tighe S. W., de Lajudie P., Dipietro K., Lindström K., Nick G., Jarvis B. D. 2000; Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50:787–801 [View Article][PubMed]
    [Google Scholar]
  24. Ueda T., Suga Y., Yahiro N., Matsuguchi T. 1995; Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences. J Bacteriol 177:1414–1417[PubMed]
    [Google Scholar]
  25. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
    [Google Scholar]
  26. Vincent J. M. 1970; The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria pp. 1–13 Edited by Vincent J. M. Oxford: Blackwell Scientific;
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  28. Yan Q. X., Wang Y. X., Li S. P., Li W. J., Hong Q. 2010; Sphingobium qiguonii sp. nov., a carbaryl-degrading bacterium isolated from a wastewater treatment system. Int J Syst Evol Microbiol 60:2724–2728 [View Article][PubMed]
    [Google Scholar]
  29. Yanni Y. G., Rizk R. Y., Corich V., Squartini A., Ninke K., Philip-Hollingsworth S., Orgambide G., De Bruijn F., Stoltzfus J. other authors 1997; Natural endophytic association between Rhizobium leguminosarum bv. trifolii and rice roots and assessment of its potential to promote rice growth. Plant Soil 194:99–114 [View Article]
    [Google Scholar]
  30. Zhang X. X., Sun L., Qiu F. B., McLean R. J. C., Jiang R. B., Song W. 2008; Rheinheimera tangshanensis sp. nov., a rice root-associated bacterium. Int J Syst Evol Microbiol 58:2420–2424 [View Article][PubMed]
    [Google Scholar]
  31. Zhang X. X., Sun L., Ma X. T., Sui X. H., Jiang R. B. 2011; Rhizobium pseudoryzae sp. nov., isolated from the rhizosphere of rice. Int J Syst Evol Microbiol 61:2425–2429 [View Article][PubMed]
    [Google Scholar]
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