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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 54, Issue 1

Phylogenetic analysis of spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S–23S rDNA internally transcribed spacer sequences Free

Abstract

The 16S rRNA gene sequences for 34 strains, including 11 isolates, were determined to classify scab-causing spp. and relatives isolated from potato scab lesions collected in Jeju, Korea. The 16S–23S rDNA internally transcribed spacer (ITS) sequences were determined to investigate whether the 16S–23S ITS region is useful for analysing intra- and interspecific relationships in these bacteria. On the basis of phylogenetic analysis of 16S rRNA gene sequences, most of the isolates were classified as and . Isolate KJO61 was placed in an ambiguous taxonomic position between and . 16S–23S ITS region sequence analysis showed that tRNA genes were not found in this region of spp. The 16S–23S ITS regions of spp. exhibited various lengths and highly variable sequence similarities (35–100 %) within strains as well as intra- and interspecies. It was revealed that could be clearly differentiated from . However, it was clarified that ITS regions are not useful in phylogenetic analysis of spp.

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Keyword(s): ITS, internally transcribed spacer
SGM
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2004-01-01
2024-04-18
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Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S–23S rDNA internally transcribed spacer sequences
Int J Syst Evol Microbiol 54, 203 (2004); https://doi.org/10.1099/ijs.0.02624-0
/content/journal/ijsem/10.1099/ijs.0.02624-0
/content/journal/ijsem/10.1099/ijs.0.02624-0
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