1887

Abstract

Twenty-nine rhizobial isolates from root nodules of and spp. growing in the Loess Plateau of China were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rRNA genes, measurement of DNA G+C content, DNA–DNA relatedness and cross-nodulation with selected legume species. Based on the results of numerical taxonomy, the isolates formed two clusters (1 and 2) with some single isolates at a similarity level of 82 %. Cluster 1 contained six isolates from and spp. Cluster 2 consisted of nine isolates from spp. DNA relatedness was greater than 80 % among isolates within cluster 2. Phylogenetic analysis based on 16S rRNA gene sequences showed that CCBAU 7190B, representing cluster 2, was closely related to and . DNA–DNA relatedness between CCBAU 7190B and reference strains of , and other related species ranged from 0 to 48·6 %. The cluster 2 isolates could also be differentiated phenotypically from related species. Based on these data, a novel species, sp. nov., is proposed for cluster 2, with the type strain CCBAU 7190B (=AS1.3401=LMG 21975).

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2003-09-01
2024-03-19
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