1887

Abstract

An obligately anaerobic, methanogenic archaeon, strain HC, was isolated from soil of the Zoige wetland on the Tibetan plateau, China. The strain was isolated through construction of an artificial butyrate-degrading consortium in co-culture with a syntrophic bacterium, ‘ subsp. ’ JCM 13344. Cells of strain HC were irregular coccoids, 0.8–2 μm in diameter, that occurred singly and utilized only H/CO for growth and methane production. Growth occurred at 18–45 °C (optimum around 37 °C). The pH for growth was 5.0–8.5 (optimal growth around pH 6.6). The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence analysis indicated that strain HC was affiliated to the genus , with sequence similarities of 94.8–97.2 % to existing members. However, strain HC was distinguished from described species by not using formate for growth or methane formation and not requiring acetate as a growth factor. On the basis of phylogenetic analysis and phenotypic characteristics, the novel species sp. nov. is proposed, with strain HC (=CGMCC 1.5146 =JCM 16311) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019273-0
2010-09-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/9/2165.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019273-0&mimeType=html&fmt=ahah

References

  1. Asakawa S., Nagaoka K. 2003; Methanoculleus bourgensis , Methanoculleus olentangyi and Methanoculleus oldenburgensis are subjective synonyms. Int J Syst Evol Microbiol 53:1551–1552 [CrossRef]
    [Google Scholar]
  2. Boone D. R., Whitman W. B. 1988; Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int J Syst Bacteriol 38:212–219 [CrossRef]
    [Google Scholar]
  3. Cheng L., Qiu T., Li X., Wang W., Deng Y., Yin X., Zhang H. 2008; Isolation and characterization of Methanoculleus receptaculi sp. nov. from Shengli oil field, China. FEMS Microbiol Lett 285:65–71 [CrossRef]
    [Google Scholar]
  4. Dianou D., Miyaki T., Asakawa S., Morii H., Nagaoka K., Oyaizu H., Matsumoto S. 2001; Methanoculleus chikugoensis sp. nov., a novel methanogenic archaeon isolated from paddy field soil in Japan, and DNA–DNA hybridization among Methanoculleus species. Int J Syst Evol Microbiol 51:1663–1669 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Furlong M. A., Singleton D. R., Coleman D. C., Whitman W. B. 2002; Molecular and culture-based analyses of prokaryotic communities from an agricultural soil and the burrows and casts of the earthworm Lumbricus rubellus . Appl Environ Microbiol 68:1265–1279 [CrossRef]
    [Google Scholar]
  7. Ganzert L., Jurgens G., Munster U., Wagner D. 2007; Methanogenic communities in permafrost-affected soils of the Laptev Sea coast, Siberian Arctic, characterized by 16S rRNA gene fingerprints. FEMS Microbiol Ecol 59:476–488 [CrossRef]
    [Google Scholar]
  8. Grabowski A., Nercessian O., Fayolle F., Blanchet D., Jeanthon C. 2005; Microbial diversity in production waters of a low-temperature biodegraded oil reservoir. FEMS Microbiol Ecol 54:427–443 [CrossRef]
    [Google Scholar]
  9. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  10. Kotelnikova S., Macario A. J. L., Pedersen K. 1998; Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater. Int J Syst Bacteriol 48:357–367 [CrossRef]
    [Google Scholar]
  11. Lai M.-C., Shu C.-M., Chen S.-C., Lai L.-J., Chiou M.-S., Hua J.-J. 2000; Methanosarcina mazei strain O1M9704, methanogen with novel tubule isolated from estuarine environment. Curr Microbiol 41:15–20 [CrossRef]
    [Google Scholar]
  12. Ma K., Liu X., Dong X. 2005; Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters. Int J Syst Evol Microbiol 55:325–329 [CrossRef]
    [Google Scholar]
  13. Maestrojuán G. M., Boone D. R., Xun L., Mah R. A., Zhang L. 1990; Transfer of Methanogenium bourgense , Methanogenium marisnigri , Methanogenium olentangyi , and Methanogenium thermophilicum to the genus Methanoculleus gen. nov., emendation of Methanoculleus marisnigri and Methanogenium , and description of new strains of Methanoculleus bourgense and Methanoculleus marisnigri . Int J Syst Bacteriol 40:117–122 [CrossRef]
    [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  15. Matthews E., Fung I. 1987; Methane emission from natural wetlands: global distribution, area, and environmental characteristics of sources. Global Biogeochem Cycles 1:61–86 [CrossRef]
    [Google Scholar]
  16. Mikucki J. A., Liu Y., Delwiche M., Colwell F. S., Boone D. R. 2003; Isolation of a methanogen from deep marine sediments that contain methane hydrates, and description of Methanoculleus submarinus sp. nov. Appl Environ Microbiol 69:3311–3316 [CrossRef]
    [Google Scholar]
  17. Owen R. J., Pitcher D. 1985; Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. In Chemical Methods in Bacterial Systematics pp 67–93 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  18. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212 [CrossRef]
    [Google Scholar]
  19. Rivard C. J., Smith P. H. 1982; Isolation and characterization of a thermophilic marine methanogenic bacterium, Methanogenium thermophilicum sp. nov. Int J Syst Bacteriol 32:430–436 [CrossRef]
    [Google Scholar]
  20. Sakai S., Imachi H., Sekiguchi Y., Ohashi A., Harada H., Kamagata Y. 2007; Isolation of key methanogens for global methane emission from rice paddy fields: a novel isolate affiliated with the clone cluster Rice Cluster I. Appl Environ Microbiol 73:4326–4331 [CrossRef]
    [Google Scholar]
  21. Spring S., Schumann P., Spröer C. 2005; Methanogenium frittonii Harris et al. 1996 is a later synonym of Methanoculleus thermophilus (Rivard and Smith 1982) Maestrojuán et al. 1990. Int J Syst Evol Microbiol 55:1097–1099 [CrossRef]
    [Google Scholar]
  22. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  25. Wu C., Liu X., Dong X. 2006; Syntrophomonas erecta subsp. sporosyntropha subsp. nov., a spore-forming bacterium that degrades short chain fatty acids in co-culture with methanogens. Syst Appl Microbiol 29:457–462 [CrossRef]
    [Google Scholar]
  26. Zhang G., Jiang N., Liu X., Dong X. 2008a; Methanogenesis from methanol at low temperatures by a novel psychrophilic methanogen, “ Methanolobus psychrophilus ” sp. nov., prevalent in Zoige wetland of the Tibetan plateau. Appl Environ Microbiol 74:6114–6120 [CrossRef]
    [Google Scholar]
  27. Zhang G., Tian J., Jiang N., Guo X., Wang Y., Dong X. 2008b; Methanogen community in Zoige wetland of Tibetan plateau and phenotypic characterization of a dominant uncultured methanogen cluster ZC-I. Environ Microbiol 10:1850–1860 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.019273-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019273-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error