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Volume 59, Issue 12

sp. nov., isolated from a culture of the Antarctic green alga Free

Abstract

Two Gram-negative, strictly aerobic bacterial strains, designated CL-AP6 and CL-AP22, were isolated from a culture of a green alga, , established from the Antarctic. Cells of the strains were straight rods and motile by means of a single polar flagellum. The strains grew with 0.5–8 % (w/v) NaCl (optimum, 1–2 %) and at 4–33 °C (optimum, 25 °C) and pH 6.5–9.1 (optimum, pH 7.5–8.1). The two strains shared 98.8 % 16S rRNA gene sequence similarity. Analysis of the 16S rRNA gene sequences of strains CL-AP6 and CL-AP22 revealed that they were members of the genus and were most closely related to C10-2 (95.5–95.8 % sequence similarity) and next to NBRC 14163 (95.1–95.5 % sequence similarity) and to other members of the genus (<95.2 % sequence similarity). Phylogenetic analyses based on the 16S rRNA gene sequences showed that strain CL-AP6 formed a robust clade with strain CL-AP22, and that this clade clustered tightly with the nearest clade containing and . The major isoprenoid quinone of strains CL-AP6 and CL-AP22 was Q-9 and the major cellular fatty acids were C 7 (40.2–41.6 %), summed feature 3 (C 7 and/or iso-C 2-OH; 26.3–26.8 %), C (13.7–13.9 %) and C (5.8–6.2 %). The genomic DNA G+C contents of strains CL-AP6 and CL-AP22 were 59.1 and 57.2 mol%, respectively. DNA–DNA hybridization experiments revealed high relatedness values (98.5±0.5 %; mean±, =2) between strains CL-AP6 and CL-AP22, indicating that the two strains constituted a single species. However, the two strains differed phenotypically from by their inability to grow without NaCl, temperature range for growth, hydrolysis of starch and production of certain enzymes. The phylogenetic analysis and physiological and biochemical data showed that strains CL-AP6 and CL-AP22 should be classified as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CL-AP6 (=KCCM 90073=JCM 15562).

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2009-12-01
2024-04-24
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Pseudomonas pelagia sp. nov., isolated from a culture of the Antarctic green alga Pyramimonas gelidicola
Int J Syst Evol Microbiol 59, 3019 (2009); https://doi.org/10.1099/ijs.0.008102-0
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Extended neighbour-joining tree based on 16S rRNA gene sequences showing the positions of strains CL-AP6 and CL-AP22 and related species. [PDF](23 KB)

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Transmission electron micrograph of a negatively stained cell of strain CL-AP6 . [PDF](265 KB)

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