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Volume 72, Issue 10

sp. nov., isolated at Marimurtra Botanical Garden in Blanes, Catalonia, Spain No Access

Abstract

In the framework of the research project called , we have isolated and characterized a bacterial plant-endophyte from the rhizomes of , hereafter referred to as strain FIT81. The bacterium is Gram negative, rod-shaped with lophotrichous flagella, and catalase- and oxidase-positive. The optimal growth temperature of strain FIT81 is 28 °C, although it can grow within a temperature range of 4–32 °C. The pH growth tolerance ranges between pH 5 and 10, and it tolerates 4% (w/v) NaCl. A 16S rRNA phylogenetic analysis positioned strain FIT81 within the genus , and multilocus sequence analysis revealed that IzPS32d, MS586, IzPS23, '' 46–2 and PS9-14 are the top five most closely related species, which were selected for further genome-to-genome comparisons, as well as for physiological and chemotaxonomic characterization. The genome size of strain FIT81 is 6 492 796 base-pairs long, with 60.6 mol% of G+C content. Average nucleotide identity and digital DNA–DNA hybridization analyses yielded values of 93.6 and 56.1%, respectively, when the FIT81 genome was compared to that of the closest type strain IzPS32d. Taken together, the obtained genomic, physiologic and chemotaxonomic data indicate that strain FIT81 is different from its closest relative species, which lead us to suggest that it is a novel species to be included in the list of type strains with the name sp. nov. (FIT81=CECT 30374=DSM 112699).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome assembly , genome-to-genome comparison , multilocus sequence analysis and Pseudomonas fitomaticsae FIT81T
Funding
This study was supported by the:
  • Ministerio de Ciencia, Innovación y Universidades-Agencia Estatal de Investigación (Spain) (Award RTC-2017-6431)
    • Principle Award Recipient: TeresaAltabella
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005557
2022-10-19
2024-04-27
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Pseudomonas fitomaticsae sp. nov., isolated at Marimurtra Botanical Garden in Blanes, Catalonia, Spain
Int J Syst Evol Microbiol 72, 005557 (2022); https://doi.org/10.1099/ijsem.0.005557
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