1887

Microbiology Society logo

Volume 141, Issue 6

Nucleotide sequences of two fimbrial major subunit genes, pmpA and ucaA, from canine-uropathogenic Proteus mirabilis strains Free

Abstract

Proteus mirabilis strains were isolated from dogs with urinary tract infection (UTI) and fimbriae were prepared from two strains. The N-terminal amino acid sequences of the major fimbrial subunits were determined and both sequences appeared identical to the N-terminal amino acid sequence of a urinary cell adhesin (UCA) (Wray, S. K., Hull, S. I., Cook, R. G., Barrish, J. Hull, R. A., 1986, Infect Immun 54, 43-49). The genes of two different major fimbrial subunits were cloned using oligonucleotide probes that were designed on the basis of the N-terminal UCA sequence. Nucleotide sequencing revealed the complete ucaA gene of 540 bp (from strain IVB247) encoding a polypeptide of 180 amino acids, including a 22 amino acid signal sequence peptide, and the pmpA (P. mirabilis P-like pili) gene of 549 bp (from strain IVB219) encoding a polypeptide of 183 amino acids, including a 23 amino acid signal sequence. Hybridization experiments gave clear indications of the presence of both kinds of fimbriae in many UTI-related canine P. mirabilis isolates. However, the presence of these fimbriae could not be demonstrated in P. vulgaris or other Proteus-related species. Database analysis of amino acid sequences of major subunit proteins revealed that the UcaA protein shares about 56% amino acid identity with the F17A and F111A major fimbrial subunits from bovine enterotoxigenic Escherichia coli. In turn, the PmpA protein more closely resembled the pyelonephritis-associated pili (Pap)-like major subunit protein from UTI-related E. coli. The evolutionary relationship of UcaA, PmpA and various other fimbrial subunit proteins is presented in a phylogenetic tree.

  • Published Online:
Keyword(s): fimbriae , major subunit gene , Proteus mirabilis and urinary tract infection
© Society for General Microbiology 1995
Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-6-1349
1995-06-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/6/mic-141-6-1349.html?itemId=/content/journal/micro/10.1099/13500872-141-6-1349&mimeType=html&fmt=ahah

References

  1. Adler J.L, Martin D.F., Finland M. 1971; Proteus infections in a general hospital. I. Biochemical characteristics and antibiotic susceptibility of the organisms.. Ann Intern Med 75:517–530
     [Google Scholar]
  2. Ahrens R., Ott M., Ritter A., Hoschützky H., Bühler T., Lottspeich F., Boulnois G.J., Jann K., Hacker J. 1993; Genetic analysis of the gene cluster encoding nonfimbrial adhesin I from an Escherichia coli uropathogen.. Infect Immun 61:2505–2512
     [Google Scholar]
  3. Allen B.L., Gerlach G.-F., Clegg S. 1991; Nucleotide sequence and functions of mrk determinants necessary for expression of type 3 fimbriae in Klebsiella pneumoniae. . J Bacteriol 173:916–920
     [Google Scholar]
  4. Allison C., Coleman N., Jones P.L., Hughes C. 1992; Ability of Proteus mirabilis to invade human urothelial cells is coupled to motility and swarming differentiation.. Infect Immun 60:4740–4746
     [Google Scholar]
  5. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990; Basic local alignment search tool.. J Mol Biol 215:403–410
     [Google Scholar]
  6. Båga M., Normark S., Hardy J., O’Hanley P., Lark D., Olsson O., Schoolnik G., Falkow S. 1983; Nucleotide sequence of the pap A gene encoding the pap pilus subunit of human uropathogenic Escherichia coli. . J Bacteriol 157:330–333
     [Google Scholar]
  7. Bahrani F.K., Mobley H.L.T. 1993; Proteus mirabilis MR/P fimbriae: molecular cloning, expression, and nucleotide sequence of the major fimbrial subunit gene.. J Bacteriol 175:457–464
     [Google Scholar]
  8. Bahrani F.K., Mobley H.L.T. 1994; Proteus mirabilis MR/P fimbrial operon: genetic organization, nucleotide sequence and conditions for expression.. J Bacteriol 176:3412–3419
     [Google Scholar]
  9. Bahrani F.K., Johnson D.E., Robbins 0., Mobley H.L.T. 1991; Proteus mirabilis flagella and MR/P fimbriae: isolation, purification, N-terminal analysis, and serum antibody response following experimental urinary tract infection.. Infect Immun 59:3574–3580
     [Google Scholar]
  10. Bahrani F.K., Cook S., Hull R.A., Massad G., Mobley H.L.T. 1993; Proteus mirabilis fimbriae: N-terminal amino acid sequence of a major fimbrial subunit and nucleotide sequences of the genes from two strains.. Infect Immun 61:884–891
     [Google Scholar]
  11. Corpet F. 1988; Multiple sequence alignment with hierarchical clustering.. Nucleic Acids Res 16:10881–10890
     [Google Scholar]
  12. Denich K., Blyn L.B., Craiu B., Braaten A., Hardy J., Low D.A., O’Hanley P.D. 1991; DNA sequences of three papA genes from uropathogenic Escherichia coli strains: evidence of structural and serological conservation.. Infect Immun 59:3849–3858
     [Google Scholar]
  13. Duguid J.P., Old D.C. 1980; Adhesive properties of Entero- bacteriaceae. . In Bacterial Adherence - Receptors and Recognition series B 6 pp. 185–217 Edited by Beachey E. H. London: Chapman & Hall;
     [Google Scholar]
  14. Felsenstein J. 1989; phylip - phylogeny inference package (version 3.2).. Cladistics 5:164–166
     [Google Scholar]
  15. Fitch W.M., Margoliash E. 1967; Construction of phylogenetic trees.. Science 155:279–284
     [Google Scholar]
  16. Gaastra W., Hansen K. 1984; Ligation of DNA with T4 DNA ligase.. In Methods in Molecular Biology pp. 225–230 Edited by Walker J. M. Clifton: Humana Press;
     [Google Scholar]
  17. Gaastra W., van Oosterom R.A.A., Pieters E.W.J., Bergmans H.E.N., van Dijk L., ten Huurne A.A.H.M. 1995; Isolation and characterisation of dog uropathogenic Proteus mirabilis strains.. Vet Microbiol (in Press)
    [Google Scholar]
  18. Garcia E., Bergmans H.E.N., van den Bosch J.F., Ørskov I., van der Zeijst B.A.M., Gaastra W. 1988a; Isolation and characterization of dog uropathogenic Escherichia coli strains and their fimbriae.. Antonie Eeeuwenhoek 54:149–163
     [Google Scholar]
  19. Garcia E., Hamers A.M., Bergmans H.E.N., van der Zeijst B.A.M., Gaastra W. 1988b; Adhesion of canine and human uropathogenic Escherichia coli and Proteus mirabilis strains to canine and human epithelial cells.. Curr Microbiol 17:333–337
     [Google Scholar]
  20. Garcia E., Bergmans H.E.N., van der Zeijst B.A.M., Gaastra W. 1992; Nucleotide sequences of the major subunits of F9 and FI 2 fimbriae of uropathogenic Escherichia coli. . Microb Pathog 13:161–166
     [Google Scholar]
  21. Hal Jones C., Jacob-Dubuisson F., Dodson K., Kuehn M., Slonim L., Striker R., Hultgren S.J. 1992; Adhesin presentation in bacteria requires molecular chaperones and ushers.. Infect Immun 60:4445–4451
     [Google Scholar]
  22. Harel J., Forget C., Saint-Amand J., Daigle F., Dubreuil D., Jacques M., Fairbrother J. 1992; Molecular cloning of a determinant coding for fimbrial antigen F1651, a Prs-like fimbrial antigen from porcine septicaemic Escherichia coli. . J Gen Microbiol 138:1495–1502
     [Google Scholar]
  23. Hultgren S.J., Normark S. 1991; Chaperone-assisted assembly and molecular architecture of adhesive pili.. Annu Rev Microbiol 45:383–415
     [Google Scholar]
  24. Johnson J.R. 1991; Virulence factors in Escherichia coli urinary tract infection.. Clin Microbiol Rev 4:80–128
     [Google Scholar]
  25. Johnson D.E., Russell R.G., Lockatell C.V., Zulty J.G, Mobley H.L.T. 1993; Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection.. Infect Immun 61:2748–2754
     [Google Scholar]
  26. Jones B.D., Lockatell C.V., Johnson D.E., Warren J.W., Mobley H.L.T. 1990; Construction of a urease-negative mutant of Proteus mirabilis-. analysis of virulence in a mouse model of ascending urinary tract infection.. Infect Immun 58:1120–1123
     [Google Scholar]
  27. Krogfelt K.A. 1991; Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli. . Rev Infect Dis 13:721–735
     [Google Scholar]
  28. Kuehn M.J., Haslam D., Normark S., Hultgren S.J. 1994; Structure, function and biogenesis of Escherichia coli P pili.. In Fimbriae: Adhesion, Genetics, Biogenesis and Vaccines pp. 37–52 Edited by Klemm P. Boca Raton: CRC Press;
     [Google Scholar]
  29. Kusters J.G., Gaastra W. 1994; Fimbrial operons and evolution.. In Fimbriae: Adhesion, Genetics, Biogenesis and Vaccines pp. 189–207 Edited by Klemm P. Boca Raton: CRC Press;
     [Google Scholar]
  30. Lintermans P. 1990 Karakterizatie van de FI7 en de Fill fimbriae van Escherichia coli en genetische analyse van de FI 7 genkluster. PhD Thesis University of Gent;
     [Google Scholar]
  31. Lintermans P., Pohl P., Deboeck F., Bertels A., Schlicker C., Vandekerckhove J., Van Damme, J., Van Montagu M., De Greve H. 1988; Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the FI 7 fimbriae in bovine enterotoxigenic Escherichia coli. . Infect Immun 56:1475–1484
     [Google Scholar]
  32. Loomes LM., Senior B.W., Kerr M.A. 1992; Proteinases of Proteus spp.: Purification, properties, and detection in urine of infected patients.. Infect Immun 60:2267–2273
     [Google Scholar]
  33. Lund B., Marklund B.-l., Stromberg N., Lindberg F., Karlsson K.-A., Normark S. 1988; Uropathogenic Escherichia coli can express serologically identical pili of different receptor binding specificities.. Mol Microbiol 2:255–263
     [Google Scholar]
  34. Marklund B.-L, Tennent J., Garcia E., Hamers A.M., Båga M., Lindberg F., Gaastra W., Normark S. 1992; Horizontal gene transfer of the E. coli pap and prs operons as a mechanism for the development of tissue specific adhesive properties.. Mol Microbiol 6:2225–2242
     [Google Scholar]
  35. Maiti S.N., DesGroseillers L., Fairbrother J.M., Harel J. 1994; Analysis of genes coding for the major and minor fimbrial subunits of the Prs-like fimbriae F165J of porcine septicemic Escherichia coli strain 4787.. Microb Pathog 16:15–25
     [Google Scholar]
  36. Massad G., Bahrani F.K., Mobley H.L.T. 1994; Proteus mirabilis fimbriae: identification, isolation, and characterization of a new ambient-temperature fimbria.. Infect Immun 62:1989–1994
     [Google Scholar]
  37. Mizunoe Y., Nakabeppu Y., Sekiguchi M., Kawabata S.-l., Moriya T., Amako K. 1988; Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens. . J Bacteriol 170:3567–3574
     [Google Scholar]
  38. Mobley H.L.T., Chippendale G.R. 1990; Hemagglutinin, urease, and hemolysin production by Proteus mirabilis from clinical sources.. J Infect Dis 161:525–530
     [Google Scholar]
  39. Mobley H.L.T., Chippendale G.R., Swihart K.G., Welch R.A. 1991; Cytotoxicity of the HpmA hemolysin and urease of Proteus mirabilis and Proteus vulgaris against cultured human renal proximal tubular epithelial cells.. Infect Immun 59:2036–2042
     [Google Scholar]
  40. Mooi F.R., van der Heide H.G.J., ter Avest A.R., Welinder K.G., Livey I., van der Zeijst B.A.M., Gaastra W. 1987; Characterization of fimbrial subunits from Bordetella species.. Microb Pathog 2:473–484
     [Google Scholar]
  41. Old D.C., Adegbola R.A. 1982; Haemagglutinins and fimbriae of Morganella, Proteus and Providencia. . J Med Microbiol 15:551–564
     [Google Scholar]
  42. Rhen M. 1985; Characterization of DNA fragments encoding fimbriae of the uropathogenic Escherichia coli strain KS71.. J Gen Microbiol 131:571–580
     [Google Scholar]
  43. Roosendaal B., Gaastra W., de Graaf F.K. 1984; The nucleotide sequence of the gene encoding the K99 subunit of enterotoxigenic Escherichia coli. . FEMS Microbiol Lett 22:253–258
     [Google Scholar]
  44. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning-, a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  45. Sareneva T., Holthöfer H., Korhonen T.K. 1990; Tissuebinding affinity of Proteus mirabilis fimbriae in the human urinary tract.. Infect Immun 58:3330–3336
     [Google Scholar]
  46. Silverblatt F.J. 1974; Host-parasite interaction in the rat renal pelvis. A possible role for pili in the pathogenesis of pyelonephritis.. J Exp Med 140:1696–1711
     [Google Scholar]
  47. Svanborg C., Ørskov F., Ørskov I. 1994; Fimbriae and disease.. In Fimbriae: Adhesion, Genetics, Biogenesis and Vaccines pp. 239–254 Edited by Klemm P. Boca Raton: CRC Press;
     [Google Scholar]
  48. Van den Bosch J.F., Hendriks J.H.I.M., Gladigau I., Willems H.M.G, Storm M. C., de Graaf F.K. 1993; Identification of Fll fimbriae on chicken Escherichia coli strains.. Infect Immun 61:800–806
     [Google Scholar]
  49. Van Die I., van Megen I., Hoekstra W.P.M., Bergmans H.E.N. 1984; Molecular organization of the genes involved in the production of F72 fimbriae causing mannose resistant hemagglutination of an uropathogenic E.coli 06: K2: HI: F7 strain.. Mol & Gen Genet 194:528–533
     [Google Scholar]
  50. Van Die I., Spierings G., van Megen I., Zuidweg E., Hoekstra W.P.M., Bergmans H.E.N. 1985; Cloning and genetic organization of the gene cluster encoding F7t fimbriae of an uropathogenic E. coli and comparison with the F72 gene cluster.. FEMS Microbiol Lett 28:329–334
     [Google Scholar]
  51. Whitney A.M., Farley M.M. 1993; Cloning and sequence analysis of the structural pilin gene of brazilian purpuric fever- associated Haemophilus influenzae biogroup aegyptius.. Infect Immun 61:1559–1562
     [Google Scholar]
  52. Wold A.E., Thorssén M., Hull S., Svanborg-Edén C. 1988; Attachment of Escherichia coli via mannose- or Galαl-4Gαl/β- containing receptors to human colonic epithelial cells.. Infect Immun 56:2531–2537
     [Google Scholar]
  53. Wold A., Caugant D.A., Lidin-Janson G., de Man P., Svanborg C. 1992; Resident colonic Escherichia coli strains frequently display uropathogenic characteristics.. J Infect Dis 165:46–52
     [Google Scholar]
  54. Wray S.K., Hull S.I., Cook R.G., Barrish J., Hull R.A. 1986; Identification and characterization of a uroepithelial cell adhesin from a uropathogenic isolate of Proteus mirabilis. . Infect Immun 54:43–49
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-6-1349
Loading
Nucleotide sequences of two fimbrial major subunit genes, pmpA and ucaA, from canine-uropathogenic Proteus mirabilis strains
Microbiology 141, 1349 (1995); https://doi.org/10.1099/13500872-141-6-1349
/content/journal/micro/10.1099/13500872-141-6-1349
/content/journal/micro/10.1099/13500872-141-6-1349
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error