1887

Abstract

A DNA clone containing a putative enolase gene was isolated from a genomic DNA library of the anaerobic fungus . It was deduced from sequence comparisons that the enolase gene was interrupted by a large 331 bp intron. The enolase gene, termed , has an ORF of 1308 bp and encodes a predicted 436 amino acid protein. The deduced amino acid sequence shows high identity (715-71%) to those of enolases from the yeasts and . The G+ C content of the enolase coding sequence (438 mol%) is considerably higher than the G + C content of the intervening sequence (142 mol%) or the 5' and 3' non-translated flanking sequences (152 and 47 mol%, respectively). The codon usage of the enolase gene was very biased as has been found for the highly expressed genes of yeast and filamentous fungi. The gene has all the canonical features (polyadenylation signal, intron splicing boundaries) of genes isolated from aerobic filamentous fungi. Only one enolase gene could be detected in genomic DNA by Southern analysis with a homologous probe. RNA analysis detected a single enolase transcript of about 16 kb. When mycelium was grown on glucose, levels of enolase mRNA were markedly increased by comparison with enolase mRNA levels in mycelium grown on cellulose, suggesting that expression of the enolase gene was transcriptionally regulated by the carbon source.

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1995-06-01
2024-03-29
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References

  1. Billon-Grand G., Fiol J.B., Breton A., Bruyère A., Oulhaj Z. 1991; DNA of some anaerobic fungi: G + C-content determi¬nation.. FEMS Microbiol Lett 82:267–270
    [Google Scholar]
  2. Brett Mason A., Buckley H.R., Gorman J.A. 1993; Molecular cloning and characterization of the Candida albicans enolase gene.. J Bacteriol 175:2632–2639
    [Google Scholar]
  3. Brownlee A.G. 1994; The nucleic acids of anaerobic fungi.. In Anaerobic Fungi Biology, Ecology and Function pp. 124–136 Mountfort D.O., Orpin C.G. Edited by New York: Marcel Dekker;
    [Google Scholar]
  4. Carmen A.A., Holland M.J. 1994; The upstream repression sequence from the yeast enolase gene EN01 is a complex regulatory element that binds multiple /rawr-acting factors including REB1.. J Biol Chem 269:9790–9797
    [Google Scholar]
  5. Chirgwin J.M., Przybyla A.E., MacDonald R.J., Rutter W.S. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.. Biochemistry 18:5294–5297
    [Google Scholar]
  6. Cohen R., Holland J.P., Yokoi T., Holland M.J. 1986; Identification of a regulatory region that mediates glucose- dependent induction of the Saccharomyces cerevisiae enolase gene EN02.. Mol Cell Biol 6:2287–2297
    [Google Scholar]
  7. Cohen R., Yokoi T., Holland J.P., Pepper A.E., Holland M.J. 1987; Transcription of the constitutively expressed yeast enolase gene EN01 is mediated by positive and negative m-acting regulatory sequences.. Mol Cell Biol 7:2753–2761
    [Google Scholar]
  8. Dessen P., Fondrat C., Valencien C., Mugnier C. 1990; Bisance: a French service for access to biomolecular sequence databases.. Cabios 6:355–356
    [Google Scholar]
  9. Edelmann S.E., Staben C. 1994; A statistical analysis of sequence features within genes from Neurospora crassa.. Exp Mycol 18:70–81
    [Google Scholar]
  10. Feinberg A.P., Vogelstein B. 1983; A technique for radio-labelling DNA restriction endonuclease fragments to high specific activity.. Anal Biochem 132:6–13
    [Google Scholar]
  11. Franklyn K.M., Warmington J.R., Otta A.K., Ashman R.B. 1990; An immunodominant antigen of Candida albicans shows homology to the enzyme enolase.. Immunol Cell Biol 68:173–178
    [Google Scholar]
  12. Gurr S.J., Unkles S.E., Kinghorn J.R. 1987; The structure and organization of nuclear genes of filamentous fungi.. In Gene Structure in Eukaryotic Microbes pp. 93–139 Kinghorn J.R. Edited by Oxford: IRL Press;
    [Google Scholar]
  13. Hébraud M., Fèevre M. 1988; Characterization of glycoside and polysaccharide hydrolases secreted by the rumen anaerobic fungi Neocallimastix frontalis, Sphaeromonas communis and Piromonas communis.. J Gen Microbiol 134:1123–1129
    [Google Scholar]
  14. Hein J. 1990; Unified approach to alignment and phylogenies.. Methods Envymol 183:626–645
    [Google Scholar]
  15. Holland M.J., Holland J.P., Thill G.P., Jackson K.A. 1981; The primary structures of two yeast enolase genes.. J Biol Chem 256:1385–1395
    [Google Scholar]
  16. Johnston P. 1988; Yeast genetics: molecular aspects.. In Yeast-, a Practical Approach pp. 107–123 Campbell I., Duffus J. Edited by Oxford: IRL Press;
    [Google Scholar]
  17. Lebioda L., Stec B., Brewer J.M. 1989; The structure of yeast enolase at 2·25-Å resolution: an 8-fold β+ α-barrel with a novel ββαα(βα)6 topology.. J Biol Chem 264:3685–3693
    [Google Scholar]
  18. Li J., Heath I.B. 1993; Chytridiomycetous gut fungi, oft overlooked contributors to herbivore digestion.. Can J Microbiol 39:1003–1013
    [Google Scholar]
  19. Lowe S.E., Theodorou M.K., Trinci A.P.J. 1987; Cellulases and xylanases of an anaerobic rumen fungus grown on wheat straw, wheat straw holocellulose, cellulose and xylan.. Appl Environ Microbiol 53:1216–1223
    [Google Scholar]
  20. McAlister L., Holland M.J. 1982; Targeted deletion of a yeast enolase structural gene.. J Biol Chem 257:7181–7188
    [Google Scholar]
  21. Mackay M. 1987; Gene organization in Plasmodium falciparum. . In Gene Structure in Eukaryotic Microbes pp. 227–241 Kinghorn J.R. Edited by Oxford: IRL Press;
    [Google Scholar]
  22. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Marvin-Sikkema F.D., Pedro Gomes T.M., Grivet J.P., Gottschal J.C., Prins R.A. 1993; Characterization of hydro- genosomes and their role in glucose metabolism of Neocallimastix sp. L2.. Arch Microbiol 160:388–396
    [Google Scholar]
  24. Moreau J., Marcand L., Maschat F., Kejzlarova-Lepesant J., Lepesant J.A., Scherrer K. 1982; A + T-rich linkers define functional domains in eukaryotic DNA.. Nature 295:260–262
    [Google Scholar]
  25. Orpin C.G. 1975; Studies on the rumen flagellate Neocallimastix frontalis.. J Gen Microbiol 91:249–262
    [Google Scholar]
  26. Read M., Hicks K.E., Sims P.F.G., Hyde J.E. 1994; Molecular characterization of the enolase gene from the human malaria parasite Plasmodium falciparum. Evidence for ancestry within a photosynthetic lineage.. Eur J Biochem 220:513–520
    [Google Scholar]
  27. Reymond P., Durand R., Hébraud M., Fèvre M. 1991; Molecular cloning of genes from the rumen anaerobic fungusNeocallimastix frontalis: expression during hydrolase induction.. FEMS Microbiol Lett 77:107–112
    [Google Scholar]
  28. Reymond P., Geourjon C., Roux B., Durand R., Fèvre M. 1992; Sequence of the phosphoenolpyruvate car boxy kinaseencoding cDNA from the rumen anaerobic fungus Neocallimastix frontalis: comparison of the amino acid sequence with animals and yeast.. Gene 110:57–63
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  30. Segil N., Shrutkowski A., Dworkin M.B., Dworkin-Rastl E. 1988; Enolase isoenzymes in adult and developing Xenopus laevis and characterization of a cloned enolase sequence.. Biochem J 251:31–39
    [Google Scholar]
  31. Sundstrom P., Aliaga G.R. 1992; Molecular cloning of cDNA and analysis of protein secondary structure of Candida albicans enolase, an abundant immunodominant glycolytic enzyme.. J Bacteriol 174:6789–6799
    [Google Scholar]
  32. Takaya N., Yanai K., Horiuchi H., Ohta A., Takagi M. 1994; Cloning and characterization of two 3’-phosphoglycerate kinase genes of Khisppus niveus and heterologous gene expression using their promoters.. Curr Genet 25:524–530
    [Google Scholar]
  33. Van der Straeten D., Rodrigues-Pousada R.A., Goodman H.M., Van Montagu M. 1991; Plant enolase: gene structure, expression and evolution.. Plant Cell 3:719–735
    [Google Scholar]
  34. Zhou L., Xue G., Orpin C.G., Black G.W., Gilbert H.J., Hazlewood G.P. 1994; Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endog- lucanase.. Biochem J 297:359–364
    [Google Scholar]
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