1887

Abstract

SUMMARY

Leucocyte influx into rabbit ileal loops, induced by strains of of different virulence, was assessed with Indium-labelled leucocytes. Strains fell into two groups on the basis of their leucotactic potential: ‘virulent’ strains (which induced fluid secretion) caused a dose-dependent leucocyte influx; strains which did not induce fluid secretion failed to induce a significant leucocyte influx. Fluid secretion was never observed in the absence of leucocyte influx, but leucocyte influx did not induce fluid secretion. The phenotype of the challenge inoculum influenced fluid secretion; young log-phase organisms induced fluid secretion with a higher frequency than overnight cultures. These findings support earlier evidence implicating leucocytes in an interactive but not exclusive role in the genesis of salmonella-induced fluid secretion. They suggest, though do not prove, that interaction of leucocytes with the appropriate phenotype of organisms results in the release of a host-derived or bacterial secretagogue, or both. The bacterial factor may or may not be the antigen related to cholera toxin, described previously.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-30-2-149
1989-10-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/30/2/medmicro-30-2-149.html?itemId=/content/journal/jmm/10.1099/00222615-30-2-149&mimeType=html&fmt=ahah

References

  1. Clarke G. J. 1988; Expression of an antigen in strains of Salmonella typhimurium which reacts with antibodies to cholera toxin. Journal of Medical Microbiology 25:139–146
    [Google Scholar]
  2. Coleman R. E., Black R. E., Welch D. M., Maxwell J. G. 1980; Indium-111 labelled leukocytes in the evaluation of suspected abdominal abscesses. American Journal of Surgery 139:99–104
    [Google Scholar]
  3. Duebbert I. E., Peterson J. W. 1985; Enterotoxin-induced fluid accumulation during experimental salmonellosis and cholera: involvement of prostaglandin synthesis by intestinal cells. Toxicon 23:157–172
    [Google Scholar]
  4. Finkelstein R. A., Marchlewicz B. A., McDonald R. J., Boesman- Finkelstein M. 1983; Isolation and characterization of a cholera-related enterotoxin from Salmonella typhimurium. FEMS Microbiology letters 17:239–241
    [Google Scholar]
  5. Giannella R. A. 1979; The importance of the intestinal inflammatory reaction in salmonella-mediated intestinal secretion. Infection and Immunity 23:140–145
    [Google Scholar]
  6. Giannella R. A., Formal S. B., Dammin G. J., Collins H. 1973; Pathogenesis of Salmonellosis. Studies of fluid secretion, mucosal invasion and morphological reaction in the rabbit ileum. Journal of Clinical Investigation 52:441–453
    [Google Scholar]
  7. Giannella R. A., Gots R. E., Charney A. N., Greenough W. B., Formal S. B. 1975; Pathogenesis of Salmonella mediated intestinal fluid secretion. Gastroenterology 69:1238–1245
    [Google Scholar]
  8. Giannella R. A., Rout W. R., Formal S. B. 1977; Effect of indomethacin on intestinal water transport in salmonella-infected rhesus monkeys. Infection and Immunity 17:136–139
    [Google Scholar]
  9. Gots R. E., Formal S. B., Giannella R. A. 1974; Indomethacin inhibition of Salmonella typhimurium, Shigella flexneri and cholera-mediated rabbit ileal secretion. Journal of Infectious Diseases 130:280–284
    [Google Scholar]
  10. Hawker R. J., Hall C. E., Drolc Z., Rhys-Evans P. H. 1985; Indium leucocyte imaging in true pyrexia of unknown origin. European Journal of Nuclear Medicine 10:172–174
    [Google Scholar]
  11. Higgs G. A., McCall E., Youlten L. J. F. 1975; A chemotactic role for prostaglandins released from polymorphonuclear leucocytes during phagocytosis. British Journal of Pharmacology 5:539–546
    [Google Scholar]
  12. Matuchansky C., Bernier J. J. 1973; Effect of prostaglandin Et on glucose, water and electrolyte absorption in human jejunum. Gastroenterology 64:1111–1118
    [Google Scholar]
  13. Pierce N. F., Carpenter C. C. J., Elliott H. L., Greenough W. B. 1971; Effects of prostaglandins, theophylline and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum. Gastroenterology 60:22–32
    [Google Scholar]
  14. Qi G.-M., Clarke G. J., Wallis T. S., Stephen J. 1989; The effect of cultural conditions on the expression of cholera-related antigen in Salmonella typhimurium. Journal of Medical Microbiology 30:213–217
    [Google Scholar]
  15. Stephen J., Wallis T. S., Starkey W. G., Candy D. C. A., Osborne M. P., Haddon S. 1985; Salmonellosis: in retrospect and prospect. In Microbial toxins and diarrhoeal disease Ciba Foundation Symposium No. 112 Pitman; London: pp 175–192
    [Google Scholar]
  16. Wallis T. S., Starkey W. G., Stephen J., Haddon S. J., Osborne M. P., Candy D. C. A. 1986a; Enterotoxin production by Salmonella typhimurium strains of different virulence. Journal of Medical Microbiology 21:19–23
    [Google Scholar]
  17. Wallis T. S., Starkey W. G., Stephen J., Haddon S. J., Osborne M. P., Candy D. C. A. 1986b; The nature and role of mucosal damage in relation to Salmonella typhimurium-induced fluid secretion in the rabbit ileum. Journal of Medical Microbiology 22:39–49
    [Google Scholar]
  18. Worton K. J., Candy D. C. A., Wallis T. S., Clarke G. J., Osborne M. P., Haddon S. J., Stephen J. 1989; Studies on early association of Salmonella typhimurium with intestinal mucosa in vivo and in vitro: relationship to virulence. Journal of Medical Microbiology 29:283–294
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-30-2-149
Loading
/content/journal/jmm/10.1099/00222615-30-2-149
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error