1887

Abstract

belongs to a large family of important pathogens, which is part of the order Spirochaetales, a distinct group of eubacteria. In order to obtain a better understanding of the genetic organization of this species, we have constructed a DNA library of the serovar , using the vector pUC13. We have isolated DNA fragments containing the genetic information required to complement strains of with defects in proline and leucine biosynthesis. While a 3.9 kb fragment which complemented also complemented , a 15 kb fragment complementing could not complement other mutations. The origin of the cloned DNA fragments was confirmed by DNA-DNA hybridization. The hybridization was specific to the pathogenic species and was not seen with the saprophytic species

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1990-04-01
2024-04-19
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References

  1. Adams E., Frank L. 1980; Metabolism of proline and the hydroxyprolines. Annual Review of Biochemistry 49:1005–1061
    [Google Scholar]
  2. Barbour A.G. 1984; Isolation and cultivation of Lyme disease spirochetes. Yale Journal of Biological Medicine 57:521–555
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Boyer A. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. Journal of Molecular Biology 41:459–472
    [Google Scholar]
  5. Brahamsha B., Greenberg E.P. 1987; Complementation of a trpE deletion in Escherichia coli by Spirochaetaaurantia DNA encoding anthranilatesynthetase component I activity. Journal of Bacteriology 169:3764–3679
    [Google Scholar]
  6. Brendle J.J., Rogul M., Alexander A.D. 1974; Deoxyribonucleic acid hybridization among selected leptospiral serotypes. International Journal of Systematic Bacteriology 24:205–214
    [Google Scholar]
  7. Casabadan M.J., Cohen S.N. 1980; Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. Journal of Molecular Biology 138:179–207
    [Google Scholar]
  8. Charon N.W., Johnson R.C., Peterson D. 1974; Amino acid biosynthesis in the spirochete Leptospira: evidence for a novel pathway of isoleucine synthesis. Journal of Bacteriology 117:203–211
    [Google Scholar]
  9. Deutcha A.H., Smith C. J., Rushlow K. E., Kretschmer P. J. 1982; Escherichia coli △1-pyrroline-5-carboxylate reductase : gene sequence, protein overproduction and purification. Nucleic Acids Research 10:7701–7714
    [Google Scholar]
  10. Deutch A.H., Rushlow K.E., Smith C.J. 1984; Analysis of the Escherichia coli proBA locus by DNA and protein sequencing. Acids Research 12:6337–6354
    [Google Scholar]
  11. Doherty J.P., Adler B., Rood J.I., Billington S.J., Faine S. 1989; Expression of two conserved leptospiral antigens in Escherichia coli. . Journal of Medical Microbiology 28:143–149
    [Google Scholar]
  12. Ellinghausen H.C., McCullough W.G. 1965; Nutrition of Leptospirapomona and growth of 13 other serotypes : fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. American Journal of Veterinarian Research 26:45–51
    [Google Scholar]
  13. Friedberg D., Rosenthal E.R., Jones J.W., Calvo J.M. 1985; Characterization of the 3’ end of the leucine operon of Salmonella typhimurium. . Molecular and General Genetics 199:486–494
    [Google Scholar]
  14. Fukunaga M., Horie I., Mifuchi I. 1989; Nucleotide sequence of a 23s ribosomal RNA gene from Leptospira interrogans serovar canicola strain Moulton. Nucleic Acids Research 17:21–23
    [Google Scholar]
  15. Hanahand D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  16. Hottinger H., Ohgi T., Zwahlen M.C., Dhamija S., Sôll D. 1987; Allele-specific complementation of an Escherichia coli leuBmutation by a LactobacilusbulgaricustRNA gene. Gene 60:75–83
    [Google Scholar]
  17. Johnson R.C. 1977; The spirochetes. Annual Review of Microbiology 31:89–106
    [Google Scholar]
  18. Johnson R.C., Gary N.D. 1962; Nutrition of Leptospirapomona.11. Fatty acid requirements. Journal of Bacteriology 85:976–982
    [Google Scholar]
  19. Johnson R.C., Harris V.G. 1967; Differentiation of pathogenic and saprophytic Leptospires: growth at low temperatures. Journal of Bacteriology 94:27–31
    [Google Scholar]
  20. Johnson R.C., Harris V, Walby J.K. 1969; Characterization of leptospires according to their fatty acid requirements. Journal of General Microbiology 55:399–407
    [Google Scholar]
  21. Lee E.C., Walker R.I., Guerry P. 1985; Expression of Campylobacter genes for proline biosynthesis in Escherichia coli. . Canadian Journal of Microbiology 31:1064–1067
    [Google Scholar]
  22. LeFebvre R.B. 1987; DNA probe for detection of the Leptospirainterrogans serovar hardjo genotype hardjo-bovis. . Journal of Clinical Microbiology 25:2336–2338
    [Google Scholar]
  23. Leisinger T. 1987; Biosynthesis of proline. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp 345–351 Neidhardt F. C., Ingraham J. L., Low K. B., Magasanik B., Schaechter M., Umbarger H. E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Messing J. 1983; New Ml3 vectors for cloning. Methods in Enzymology. 101C:20–78
    [Google Scholar]
  26. Miller J.H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Parsot C. 1986; Evolution of biosynthetic pathways: a common ancestor for threonine synthase, threonine dehydratase and D-serine dehydratase. EMBO Journal 5:3013–3019
    [Google Scholar]
  28. Paster B.J., Stackebrandt E., Hespell R.B., Hahn C.M., Woese C.R. 1984; The phylogeny of the spirochetes. Systematic and Applied Microbiology 5:337–351
    [Google Scholar]
  29. Piggot P.J., Hoch J.A. 1985; Revised genetic linkage map of Bacillus subtilis. . Microbiological Reviews 49:158–179
    [Google Scholar]
  30. Rambach A., Hogness D.S. 1977; Translation of Drosophila melanogaster sequences in Escherichia coli. . Proceedings of the National Academy of Sciences of the United States of America 74:5041–5045
    [Google Scholar]
  31. Roberts R.B., Abelson P.H., Cowie D.B., Bolton E.T., Britten R.J. 1955; Studies of biosynthesis in Escherichia coli. Carnegie Institution of Washington publication 607
    [Google Scholar]
  32. Sanderson K.E., Roth J.R. 1988; Linkage map of Salmonella typhimurium,. , VII. Microbiological Reviews 52:485–532
    [Google Scholar]
  33. Shapira S.K., Chou J., Richaud F.V., Casadaban M.J. 1983; New versatile plasmid vectors for expression of hybrid protein coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of β-galactosidase. Gene 25:71–82
    [Google Scholar]
  34. Silhavy T.J., Berman M.L., Enquist L.W. 1984 Experiments with Gene Fusions. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Smith C.J., Deutch A.H., Rushlow K.E. 1984; Purification and characterization of a γ-glutamyl kinase involved in Escherichia coliproline synthesis. Journal of Bacteriology 157:545–551
    [Google Scholar]
  36. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  37. Umbarger H.E. 1987; Biosynthesis of the branched-chain amino- acids. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp 352–367 Neidhardt F. C., Ingraham J.I., Low K. B., Magasanik B., Schaechter M., Umbarger H.E. Edited by Washington, DC: American Society for Microbiolog);
    [Google Scholar]
  38. Van Eys G.J.J.M., Zaal J., Schoone G.J., Terpstra W.J. 1988; DNA hybridization with hardjobovis-specific recombinant probes as a method for type discrimination of Leptospirainterrogansserovarhardjo. . Journal of General Microbiology 134:567–574
    [Google Scholar]
  39. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7- derived system for insertion mutagenesis and sequencing universal primers. Gene 19:259–261
    [Google Scholar]
  40. Westfall H.N. 1980; Isoleucine biosynthetic pathways in Leptospirainterrogans. . PhD thesis West Virginia University:
    [Google Scholar]
  41. Westfall H.N., Charon N.W., Peterson D.E. 1983; Multiple pathways for isoleucine biosynthesis in the spirochete Leptospira. . Journal of Bacteriology 154:846–853
    [Google Scholar]
  42. Yamaguchi T., Ono E., Yanagawa R. 1988; Expression of antigen genes of Leptospirainterrogans serovar canicola in Escherichia coli. . Microbiology and Immunology 32:1179–1187
    [Google Scholar]
  43. Yelton D.B., Charon N.W. 1984; Cloning of a gene required for tryptophan biosynthesis from Leptospirabiflexa serovarpatoc into Escherichia coli. . Gene 28:147–152
    [Google Scholar]
  44. Yelton D.B., Cohen R.A. 1986; Analysis of cloned DNA from Leptospirabiflexa serovarpatoc which complements a deletion of the LeptospiratrpE gene. Journal of Bacteriology 165:41–46
    [Google Scholar]
  45. Yelton D.B., Peng S.L. 1989; Identification and nucleotide sequence of the Leptospirabiflexa serovar patoctrpEand trpGgenes. Journal of Bacteriology 171:2083–2089
    [Google Scholar]
  46. Zuerner R.L., Bolin C.A. 1988; Repetitive sequence element cloned from Leptospirainterrogansserovarhardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis. Journal of Clinical Microbiology 26:2495–2500
    [Google Scholar]
  47. Zuerner R.L., Charon N.W. 1988; Nucleotide sequence analysis of a gene cloned from Leptospirabiflexaserovarpatoc which complements an argE defect in Escherichia coli. . Journal of Bacteriology 170:4548–4554
    [Google Scholar]
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