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Volume 136, Issue 12

The effects of osmotic upshock on the intracellular solute pools of Free

Abstract

The effects of hypersaline treatment (osmotic upshock) on solute accumulation have been studied in the Gram-positive bacterium Natural abundance C NMR spectroscopy studies revealed only proline as a major organic osmoticum in cells grown in defined medium (no exogenous organic solutes) and this finding was confirmed by amino acid analysis. Intracellular concentrations of both K and proline rose markedly after osmotic upshock. K influx from the medium was rapid (< 1 h) but proline synthesis was a slower process (5–9 h). Proline synthesis appeared to be dependent on the prior accumulation of K and it is possible that K serves in some manner as the signal for increased proline synthesis. In cells upshocked in medium enriched in glycine betaine the endogenous synthesis of proline was repressed and glycine betaine served as the sole organic osmoticum. K was also accumulated under these conditions.

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© Society for General Microbiology 1990
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1990-12-01
2024-04-19
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References

  1. Anderson C. B., Witter L. D. 1982; Glutamine and proline accumulation by Staphylococcus aureus with reduction in water activity. Applied and Environmental Microbiology 43:1501–1503
     [Google Scholar]
  2. Bates L. S., Waldren R. P., Teare I. D. 1973; Rapid determination of free proline for water stress studies. Plant and Soil 39:205–207
     [Google Scholar]
  3. Booth I. R., Cairney J., Sutherland L., Higgins C. F. 1988; Enteric bacteria and osmotic stress: an integrated homeostatic system. Journal of Applied Bacteriology Symposium Supplement35S–39S
     [Google Scholar]
  4. Brown A. D. 1976; Microbial water stress. Bacteriological Reviews 40:803–846
     [Google Scholar]
  5. Brown A. D., Simpson J. R. 1972; Water relations of sugar tolerant yeasts: the role of intracellular polyols. Journal of General Microbiology 72:589–591
     [Google Scholar]
  6. Chudek J. A., Foster R., Moore D., Reed R. H. 1987; Identification and quantification of methylated osmolytes in algae using proton nuclear magnetic resonance spectroscopy. British Phycological Journal 22:169–173
     [Google Scholar]
  7. Csonka L. N. 1989; Physiological and genetic responses of bacteria to osmotic stress. Microbiological Reviews 53:121–147
     [Google Scholar]
  8. Dinnibier U., Limpinsel E., Schmid R., Barker E. P. 1988; Transient accumulation of potassium glutamate and its replacement by trehalose during adaption of growing cells of Escherichia coli to elevated sodium chloride concentrations. Archives of Microbiology 150:348–357
     [Google Scholar]
  9. Epstein W. 1986; Osmoregulation by potassium transport in Escherichia coli . FEMS Microbiology Reviews 39:73–78
     [Google Scholar]
  10. Gadd G. M., Chudek J. A., Foster R., Reed R. H. 1984; The osmotic responses of Penicillium ochro-chloron: changes in internal solute levels in response to copper and salt stress. Journal of General Microbiology 130:1969–1975
     [Google Scholar]
  11. Giaever H. M., Stryvold O. B., Kaasen I., Strom A. R. 1988; Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli . Journal of Bacteriology 170:2841–2849
     [Google Scholar]
  12. Gould G. W., Measures J. C. 1977; Water relations in single cells. Philosophical Transactions of the Royal Society of London 278:151–166
     [Google Scholar]
  13. Hayzer D. J., Leisinger T. 1980; The gene-enzyme relationships of proline biosynthesis in Escherichia coli . Journal of General Microbiology 118:287–293
     [Google Scholar]
  14. Hecker M., Heim M., Volker U., Wolfel L. 1988; Induction of stress proteins by sodium chloride treatment in Bacillus subtilis . Archives of Microbiology 150:564–566
     [Google Scholar]
  15. Higgins C. F., Cairney J., Stirling J. A., Sutherland L., Booth I. R. 1987; Osmotic regulation of gene expression: ionic strength as an intracellular signal?. Trends in Biochemical Sciences 12:339–344
     [Google Scholar]
  16. Higgins C. F., Dorman C. J., Stirling J. A., Waddell L., Booth I. R., May G., Bremer E. 1988; A physiological role for DNA supercoiling in the osmotic regulation of gene expression in Salmonella typhimurium and Escherichia coli . Cell 52:569–584
     [Google Scholar]
  17. Hutkins R. W., Gilefson W. L., Kashket E. R. 1987; Betaine transport imparts osmotolerance on a strain of Lactobacillus acidophilus . Applied and Environmental Microbiology 53:2275–2281
     [Google Scholar]
  18. Imhoff J. F. 1986; Osmoregulation and compatible solutes in eubacteria. FEMS Microbiology Reviews 39:57–66
     [Google Scholar]
  19. Imhoff J. F., Rodriguez-Valera F. 1984; Betaine is the main compatible solute of halophilic eubacteria. Journal of Bacteriology 160:478–479
     [Google Scholar]
  20. Killham K., Firestone M. K. 1984; Salt stress control of intracellular solutes in Streptomyces indigenous to saline soils. Applied and Environmental Microbiology 47:301–306
     [Google Scholar]
  21. Krishna R. V., Leisinger T. 1979; Biosynthesis of proline in Pseudomonas aeuruginosa . Biochemical Journal 181:215–222
     [Google Scholar]
  22. Larsen P. I., Sydnes L. K., Landfald B., Strom A. R. 1987; Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid and trehalose. Archives of Microbiology 147:1–7
     [Google Scholar]
  23. Measures J. C. 1975; Role of amino acids in osmoregulation of non-halophilic bacteria. Nature; London: 257398–400
     [Google Scholar]
  24. Reed R. H., Stewart W. D. P. 1985; Evidence for turgor sensitive K+ influx in the cyanobacteria Anabaena variabilis ATCC 29413 and Synechocystis PCC 6714. Biochimica et Biophysica Acta 812:155–162
     [Google Scholar]
  25. Reed R. H., Collins J. C., Russell G. 1980; The effects of salinity upon galactosyl-glycerol content and concentration of the marine red alga Porphyra purpurea (Roth) C. Ag. Journal of Experimental Botany 31:1539–1554
     [Google Scholar]
  26. Reed R. H., Davison I. R., Chudek J. A., Foster R. 1985a; The osmotic role of mannitol in the Phaeophyta: an appraisal. Phycologia 24:35–47
     [Google Scholar]
  27. Reed R. H., Warr S. R. C., Richardson D. L., Moore D. J., Stewart W. D. P. 1985b; Multiphasic osmotic adjustment in a euryhaline cyanobacterium. FEMS Microbiology Letters 28:225–229
     [Google Scholar]
  28. Richey B., Scottcayley D., Mossing M. C., Kalka C., Anderson C. F., Farrar T. C., Record M. 1987; Variability of the intracellular ionic environment of Escherichia coli . Journal of Biological Chemistry 262:7157–7164
     [Google Scholar]
  29. Sutherland L., Cairney J., Elmore M. J., Booth I. R., Higgins C. F. 1986; Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium. Journal of Bacteriology 168:805–814
     [Google Scholar]
  30. Tempest D. W., Meers J. L., Brown C. M. 1970; Influence of environment on the content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
     [Google Scholar]
  31. Tomenchok D. M., Brandriss M. C. 1987; Gene-enzyme relationships in the proline biosynthetic pathway of Saccharomyces cerevisiae . Journal of Bacteriology 169:5364–5372
     [Google Scholar]
  32. Warr S. R. C., Reed R. H., Stewart W. D. P. 1985; Carbohydrate accumulation in osmotically stressed cyanobacteria (blue-green algae): interactions of temperature and salinity. New Phytologist 100:285–292
     [Google Scholar]
  33. Warr S. R. C., Reed R. H., Stewart W. D. P. 1988; The compatibility of osmotica in cyanobacteria. Plant Cell and Environment 11:137–142
     [Google Scholar]
  34. Whatmore A. M. 1989 Osmotic responses of Bacillus subtilis MSc thesis University of Dundee, UK:
     [Google Scholar]
  35. Whatmore A. M., Reed R. H. 1990; Determination of turgor pressure of Bacillus subtilis: a possible role for K+ in turgor regulation. Journal of General Microbiology 136:2521–2526
     [Google Scholar]
  36. Wohlfarth A., Severin J., Galinski E. A. 1990; The spectrum of compatible solutes in heterotrophic halophilic eubacteria of the family Halomonadaceae . Journal of General Microbiology 136:705–712
     [Google Scholar]
  37. Yoshinaga F., Tsuchida T., Okumura S. 1975; Purification and properties of glutamyl kinases for l-proline and Ll-glutamine biosynthesis in Brevibacterium flavum . Agricultural and Biological Chemistry 39:1269–1273
     [Google Scholar]
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The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis
Microbiology 136, 2527 (1990); https://doi.org/10.1099/00221287-136-12-2527
/content/journal/micro/10.1099/00221287-136-12-2527
/content/journal/micro/10.1099/00221287-136-12-2527
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