1887

Abstract

A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek′s disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek′s disease. Chickens were immunized at 1 day old with 10 p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek′s disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production.

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2002-08-01
2024-04-19
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References

  1. Abujoub A., Coussens P. M. 1995; Development of a sustainable chick cell line infected with Marek′s disease virus. Virology 214:541–549
    [Google Scholar]
  2. Abujoub A. A., Coussens P. M. 1997; Evidence that Marek′s disease virus exists in a latent state in a sustainable fibroblast cell line. Virology 229:309–321
    [Google Scholar]
  3. Benton W. J., Cover M. S. 1957; The increased incidence of visceral lymphomatosis in broiler and replacement birds. Avian Disease320–327
    [Google Scholar]
  4. Biggs P. M. 2001; The history and biology of Marek’s Disease Virus. In Marek’s Disease pp 1–25 Edited by Kanji H. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  5. Brunovskis P., Velicer L. F. 1995; The Marek′s disease virus (MDV) unique short region: alphaherpesvirus-homologous, fowlpox virus-homologous, and MDV-specific genes. Virology 206:324–338
    [Google Scholar]
  6. Calnek B. W. 2001; Pathogenesis of Marek′s disease virus infection. In Marek’s Disease pp 25–55 Edited by Kanji H. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  7. Calnek B. W., Schat K. A., Peckham M. C., Fabricant J. 1983; Field trials with a bivalent vaccine (HVT and SB-1) against Marek′s disease. Avian Disease 27:844–849
    [Google Scholar]
  8. Dorange F., Tischer B. K., Vautherot J. F., Osterrieder N. 2002; Characterization of Marek′s disease virus serotype 1 (MDV-1) deletion mutants that lack UL46 to UL49 genes: MDV-1 UL49, encoding VP22, is indispensable for virus growth. Journal of Virology 76:1959–1970
    [Google Scholar]
  9. Jaikumar D., Read K. M., Tannock G. A. 2001; Adaptation of Marek′s disease virus to the Vero continuous cell line. Veterinary Microbiology 79:75–82
    [Google Scholar]
  10. Lee L. F., Wu P., Sui D., Ren D., Kamil J., Kung H. J., Witter R. L. 2000; The complete unique long sequence and the overall genomic organization of the GA strain of Marek′s disease virus. Proceedings of the National Academy of Sciences, USA 97:6091–6096
    [Google Scholar]
  11. Mettenleiter T. C. 2002; Herpesvirus assembly and egress. Journal of Virology 76:1537–1547
    [Google Scholar]
  12. Morgan R. W., Cantello J. L., McDermott C. H. 1990; Transfection of chicken embryo fibroblasts with Marek′s disease virus DNA. Avian Disease 34:345–351
    [Google Scholar]
  13. Osterrieder N., Wagner R., Brandmüller C., Schmidt P., Wolf H., Kaaden O. R. 1995; Protection against EHV-1 challenge infection in the murine model after vaccination with various formulations of recombinant glycoprotein gp14 (gB). Virology 208:500–510
    [Google Scholar]
  14. Rispens B. H., van Vloten H., Mastenbroek N., Maas H. J., Schat K. A. 1972a; Control of Marek′s disease in the Netherlands. I. Isolation of an avirulent Marek′s disease virus (strain CVI 988) and its use in laboratory vaccination trials. Avian Disease 16:108–125
    [Google Scholar]
  15. Rispens B. H., van Vloten H., Mastenbroek N., Maas J. L., Schat K. A. 1972b; Control of Marek′s disease in the Netherlands. II. Field trials on vaccination with an avirulent strain (CVI 988) of Marek′s disease virus. Avian Disease 16:126–138
    [Google Scholar]
  16. Saiki R. K., Bugawan T. L., Horn G. T., Mullis K. B., Erlich H. A. 1986; Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature 324:163–166
    [Google Scholar]
  17. Schat K. A., Calnek B. W., Fabricant J. 1982; Characterisation of two highly oncogenic strains of Marek′s disease virus. Avian Pathology 593–605
    [Google Scholar]
  18. Schumacher D., Tischer B. K., Fuchs W., Osterrieder N. 2000; Reconstitution of Marek′s disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant. Journal of Virology 74:11088–11098
    [Google Scholar]
  19. Schumacher D., Tischer B. K., Reddy S. M., Osterrieder N. 2001; Glycoproteins E and I of Marek′s disease virus serotype 1 are essential for virus growth in cultured cells. Journal of Virology 75:11307–11318
    [Google Scholar]
  20. Tischer B. K., Schumacher D., Messerle M., Wagner M., Osterrieder N. 2002; The products of the UL10 (gM) and the UL49.5 genes of Marek′s Disease Virus serotype 1 (MDV-1) are essential for virus growth in cultured cells. Journal of General Virology 83:997–1003
    [Google Scholar]
  21. Tulman E. R., Afonso C. L., Lu Z., Zsak L., Rock D. L., Kutish G. F. 2000; The genome of a very virulent Marek′s disease virus. Journal of Virology 74:7980–7988
    [Google Scholar]
  22. Witter R. L. 1983; Characteristics of Marek′s disease viruses isolated from vaccinated commercial chicken flocks: association of viral pathotype with lymphoma frequency. Avian Disease 27:113–132
    [Google Scholar]
  23. Witter R. L. 1992 Safety and comparative efficacy of the CVI988/Rispens vaccine strain World′s Poultry Science Association; Amsterdam:315–319
    [Google Scholar]
  24. Witter R. L. 1997; Increased virulence of Marek′s disease virus field isolates. Avian Disease 41:149–163
    [Google Scholar]
  25. Witter R. L. 2001; Protective efficacy of Marek′s disease vaccines. In Marek′s Disease pp 57–90 Edited by Hirai K. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  26. Witter R. L., Lee L. F., Fadly A. M. 1995; Characteristics of CVI988/Rispens and R2/23, two prototype vaccine strains of serotype 1 Marek′s disease virus. Avian Disease 39:269–284
    [Google Scholar]
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