1887

Abstract

A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription , although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.

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1993-07-01
2024-04-19
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References

  1. Akkina RK, Richardson JC, Aguilera MC, Yang C. 1991; Heterogeneous forms of polymerase proteins exist in influenza A virus-infected cells.. Virus Research 19:17–30
    [Google Scholar]
  2. Bandzilius RJ, Swanson MS, Dreyfuss G. 1989; RNA-binding proteins as developmental regulators.. Genes and Development 3:431–437
    [Google Scholar]
  3. Braam J, Ulmanen I, Krug RM. 1983; Molecular model of a eucaryotic transcription complex: functions and movements of influenza P proteins during capped RNA-primed transcription.. Cell 34:609–618
    [Google Scholar]
  4. Desselberger U, Racaniello VR, Zazra JJ, Palese P. 1980; The 3ʹ and 5ʹ terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity.. Gene 8:315–328
    [Google Scholar]
  5. Digard P, Blok VC, Inglis SC. 1989; Complex formation between influenza virus polymerase proteins expressed in Xenopus oocytes.. Virology 171:162–169
    [Google Scholar]
  6. Galarza JM, Sowa A, Hill VM, Szewczyk M, Skorko R, Summers DF. 1991; Expression of influenza A virus RNA polymerase subunits and NP by recombinant baculoviruses and the biochemical activities of these proteins.. In Eighth International Conference on Negative Strand Viruses, Charleston, S.C., U.S.A.September 1991: p. 64
    [Google Scholar]
  7. Ishihama A, Nagata K. 1988; Viral RNA polymerases. CRC Critical Reviews in Biochemistry 23:27–76
    [Google Scholar]
  8. Jones IM, Reay PA, Philpott KL. 1986; Nuclear location of all three influenza polymerase proteins and a nuclear signal in polymerase PB2.. EMBO Journal 5:2371–2376
    [Google Scholar]
  9. Kingsbury DW, Jones IM, Murti KG. 1987; Assembly of influenza ribonucleoprotein in vitro using recombinant nucleo-protein.. Virology 156:396–403
    [Google Scholar]
  10. Kobayashi M, Tuchiya K, Nagata K, Ishihama A. 1992; Reconstitution of influenza virus RNA polymerase from three subunits expressed using recombinant baculovirus system.. Virus Research 22:235–245
    [Google Scholar]
  11. Krug RM, Ueda M, Palese P. 1975; Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis.. Journal of Virology 16:790–796
    [Google Scholar]
  12. Lamb RA, Choppin PW. 1976; Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription.. Virology 74:504–519
    [Google Scholar]
  13. Lamb RA, Choppin PW. 1983; The gene structure and replication of influenza virus.. Annual Review of Biochemistry 52:467–506
    [Google Scholar]
  14. Mahy BWJ, Barrett T, Nichol ST, Penn CR, Wolsten-holme AJ. 1981; Analysis of the functions of influenza virus genome RNA segments by use of temperature-sensitive mutants of fowl plague virus.. In In The Replication of Negative Stranded Viruses pp 379–387Edited by Bishop DHL, Compans RW. New York: Elsevier;
    [Google Scholar]
  15. McCauley JW, Mahy BWJ. 1983; Structure and function of the influenza virus genome.. Biochemical Journal 211:281–294
    [Google Scholar]
  16. Poch O, Sauvaget I, Delarue M, Tordo N. 1989; Identification of four conserved motifs among the RNA-dependent polymerase encoding elements.. EMBO Journal 8:3867–3874
    [Google Scholar]
  17. Robertson JS. 1979; 5ʹ and 3ʹ terminal nucleotide sequences of the RNA genome segments of influenza virus.. Nucleic Acids Research 6:3745–3757
    [Google Scholar]
  18. Schimmel PR, Budzik GP. 1977; Photo-cross-linking of protein-nucleic acid complexes.. Methods in Enzymology 46:168–180
    [Google Scholar]
  19. Seong BL, Brownlee GG. 1992a; A new method for reconstituting influenza polymerase and RNA in vitro: a study of promoter elements for cRNA and vRNA synthesis in vitro and viral rescue in vivo.. Virology 186:247–260
    [Google Scholar]
  20. Seong BL, Brownlee GG. 1992b; Nucleotides 9 to 11 of the influenza A virion RNA promoter are crucial for activity in vitro.. Journal of General Virology 73:3115–3124
    [Google Scholar]
  21. Ulmanen I., Broni BA, Krug RM. 1981; Role of two of the influenza virus core P proteins in recognizing cap 1 structures (m7GpppNm) on RNAs and initiating viral RNA transcription.. Proceedings of the National Academy of Sciences, U.S.A. 78:7355–7359
    [Google Scholar]
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