1887

Abstract

SUMMARY

Viroid-like RNA was isolated from some samples of grapevine leaves ( L.). When inoculated to cucumber, this RNA induced symptoms that were identical to those induced by hop stunt viroid (HSV). It had a molecular size similar to that of HSV, and hybridized with a P-labelled DNA probe for the cucumber isolate of HSV (HSV-c, pCP-55). This hybridization suggests high (> 55%) sequence homology with HSV. The HSV-related sequence was detected not only in the infected cucumber plants but also directly in RNA extracted from grapevine plants. The result indicates that viroid-like RNA, closely related to HSV, is present in grapevines in Japan.

Keyword(s): grapevine , hop stunt viroid and viroid
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/content/journal/jgv/10.1099/0022-1317-66-2-333
1985-02-01
2024-04-20
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References

  1. Carmichael G. G., Mcmaster G. K. 1980; The analysis of nucleic acids in gels using glyoxal and acridine orange. Methods in Enzymology 65:380–391
    [Google Scholar]
  2. Diener T. O. 1979 Viroids and Viroid Diseases New York: Academic Press;
    [Google Scholar]
  3. Iri M., Shimura T., Togawa H., Ueno K. 1982; Elimination of grapevine leafroll virus by heat treatment and meristem culture. Annals of the Phytopathological Society of Japan 48:685–687
    [Google Scholar]
  4. Ishikawa M., Meshi T., Ohno T., Okada V., Sano T., Uyeda L., Shikata E. 1984; A revised replication cycle for viroid: the role of longer than unit length RNA in viroid replication. Molecular and General Genetics (in press)
    [Google Scholar]
  5. Kiefer M. C., Owens R. A., Diener T. O. 1983; Structural similarities between viroids and transposable genetic elements. Proceedings of the National Academy of Sciences, U.S.A 80:6234–6238
    [Google Scholar]
  6. Loening U.E. 1967; The determination of the molecular weight of ribonucleic acid by polyacrylamide gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982; Labelling the 5′ end of DNA with T4 polynucleotide kinase. pp 122–123 In Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Ohno T., Takamatsu N., Meshi T., Okada Y. 1983; Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy. Nucleic Acids Research 11:6185–6197
    [Google Scholar]
  9. Sanger H. L. 1982; Biology, structure, functions and possible origin of viroids. In Encyclopedia of Plant Physiology, New Series vol 14B: pp 368–454 Edited by Parthier B., Boulter D. Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  10. Sano T., Uyeda I., Shikata E., Ohno T., Okada Y. 1984; Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid. Nucleic Acids Research 12:3427–3434
    [Google Scholar]
  11. Sasaki M., Shikata E. 1977a; Studies on the host range of hop stunt disease in Japan. Proceedings of the Japan Academy 55B:103–108
    [Google Scholar]
  12. Sasaki M., Shikata E. 1977b; On some properties of hop stunt disease agent, a viroid. Proceedings of the Japan Academy 55B:109–112
    [Google Scholar]
  13. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  14. Uyeda I., Sano T., Shikata E. 1984; Purification of cucumber pale fruit viroid. Annals of the Phytopathological Society of Japan 50:331–338
    [Google Scholar]
  15. Visvader J. E., Symons R. H. 1983; Comparative sequence and structure of different isolates of citrus exocortis viroid. Virology 130:232–237
    [Google Scholar]
  16. Yamamoto H., Kagami Y., Kurokawa M., Nishimura S., Kubo S., Inoue M., Murayama D. 1970; Studies On hop stunt disease I. Memoirs of the Faculty of Agriculture, Hokkaido University 7:491–512
    [Google Scholar]
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