1887

Abstract

SUMMARY

Western equine encephalitis virus, and RNA virus, and herpes simplex virus type 1, a DNA virus, were efficiently inactivated in less than 1 min by exposure to long-wave ultraviolet light (320 to 380 nm) in the presence of several psoralen derivatives. The psoralen photochemical reaction was chosen for study due to its known specificity for nucleic acids. Neither the light nor any of the drugs alone caused appreciable inactivation. The inactivation kinetics and dependence on light intensity and on different derivatives of psoralen were studied. The high solubility of a new aminomethyl psoralen derivative was found to be advantageous in the photochemical inactivation of the RNA virus, but was not in the case of the more easily inactivated DNA virus. Within its limited solubility range trimethylpsoralen was superior to its aminomethyl derivative on a molar basis for the inactivation of both types of viruses under most of the conditions studied.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-2-345
1978-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/2/JV0400020345.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-2-345&mimeType=html&fmt=ahah

References

  1. Averbeck D., Moustacchi E. 1975; 8-Methoxypsoralen plus 365 nm light effects and repair in yeast. Biochimica et Biophysica Acta 395:393–404
    [Google Scholar]
  2. Baccichetti F., Bordin F., Marciani S., Dall’Acqua F., Rodighiero G. 1975; Contribution of monofunctional adducts formed by furocoumarins with DNA to the inhibition of nucleic acids synthesis. Zeitschrift für Naturforschung 31c:207–208
    [Google Scholar]
  3. Bachrach H. L. 1966; Reactivity of viruses in vitro . Progress in Medical Virology 8:214–313
    [Google Scholar]
  4. Baden H. P., Parrington J. M., Delhanty J. D. A., Pathak M. A. 1971; DNA synthesis in normal and xeroderma pigmentosum fibroblasts following treatment with 8-methoxypsoralen and long wave ultraviolet light. Biophysica et Biochimica Acta 262:247–255
    [Google Scholar]
  5. Ben-Hur E., Elkind M. M. 1973; Psoralen plus near ultraviolet light inactivation of cultured Chinese hamster cells and its relation to DNA cross-links. Mutation Research 18:315–324
    [Google Scholar]
  6. Bordin F., Marciani S., Baccichetti F. R., Dall’Acqua F., Rodighiero G. 1975; Studies on the photosensitizing properties of angelicin, an angular furocoumarin forming only monofunctional adducts with the pyrimidine bases of DNA. Italian Journal of Biochemistry 24:258–267
    [Google Scholar]
  7. Bordin F., Carlassare F., Baccichetti F., Anselmo L. 1976; DNA repair and recovery in Escherichia coli after psoralen and angelicin photosensitization. Biochimica et Biophysica Acta 447:249–259
    [Google Scholar]
  8. Cech T., Pardue M. L. 1977; Cross-linking of DNA with trimethylpsoralen is a probe for chromatin structure. Cell 11:631–640
    [Google Scholar]
  9. Colburn N. H., Boutwell R. K. 1968; The in vivo binding of beta-propiolactone to mouse skin DNA, RNA and protein. Cancer Research 28:642–652
    [Google Scholar]
  10. Cole R. S. 1971; Psoralen monoadducts and interstrand cross-links in DNA. Biochimica et Biophysica Acta 254:30–39
    [Google Scholar]
  11. Cole R. S., Levitan D., Sinden R. R. 1976; Removal of psoralen interstrand cross-links from DNA of Escherichia coli: mechanism and genetic control. Journal of Molecular Biology 103:39–59
    [Google Scholar]
  12. Dall’Acqua F., Marciani S., Ciavatta L., Rodighiero G. 1971; Formation of inter-strand cross-linkings in the photoreactions between furocoumarins and DNA. Zeitschrift für Naturforschung 26b:561–569
    [Google Scholar]
  13. Day R. S., Giuffrida A. S., Dingman C. W. 1975; Repair by human cells of adenovirus-2 damaged by psoralen plus near ultraviolet light treatment. Mutation Research 33:311–320
    [Google Scholar]
  14. Gilchrest B. A., Parrish J. A., Tanenbaum L., Haynes H. A., Fitzpatrick T. B. 1976; Oral methoxsalen photochemotherapy of mycosis fungoides. Cancer 38:683–389
    [Google Scholar]
  15. Graham F. L., Abrahams P. J., Mulder C., Heijneker H. L., Warnaar S. O., Devries F. A. J., Fiers W., Van Dereb A. J. 1974; Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40. Cold Spring Harbor Symposia on Quantitative Biology 39:637–650
    [Google Scholar]
  16. Hanson C. V., Shen C.-K. J., Hearst J. E. 1976; Cross-linking of DNA in situ as a probe for chromatin structure. Science 193:62–64
    [Google Scholar]
  17. Hearst J. E., Thiry L. 1977; The photoinactivation of an RNA virus, vesicular stomatitis virus, with the aid of newly synthesized psoralen derivatives. Nucleic Acids Research 4:1339–1347
    [Google Scholar]
  18. Hyde J. E., Hearst J. E. 1978; The binding of psoralen derivatives to DNA and chromatin: influence of the ionic environment on dark binding and photoreactivity. Biochemistry 17:1251–1256
    [Google Scholar]
  19. Isaacs S. T., Shen C.-K. J., Hearst J. E., Rapoport H. 1977; Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA. Biochemistry 16:1058–1964
    [Google Scholar]
  20. Marciani S., Terbojevich M., Dall’Acqua F., Rodighiero G. 1973; Bifunctional photobinding of psoralen to single stranded nucleic acids. Zeitschrift für Naturforschung 28c:370–375
    [Google Scholar]
  21. Musajo L., Rodighiero G. 1962; The skin-photosensitizing furocoumarins. Experientia 18:153–162
    [Google Scholar]
  22. Musajo L., Rodighiero G., Colombo J., , Torlone Dall’Acqua F. 1965; Photosensitizing furocoumarins: interactions with DNA and photoinactivation of DNA containing viruses. Experientia 21:22–24
    [Google Scholar]
  23. Parrish J. A., Fitzpatrick T. B., Tanenbaum L., Pathak M. A. 1974; Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. New England Journal of Medicine 291:1207–1211
    [Google Scholar]
  24. Pathak M. A., Kramer D. M. 1969; Photosensitization of skin in vivo by furocoumarins (psoralens). Biochimica et Biophysica Acta 195:197–206
    [Google Scholar]
  25. Pathak M. A., Kramer D. M., Fitzpatrick T. B. 1974; Photobiology and photochemistry of furocoumarins (psoralens). In Sunlight and Man: Normal and Abnormal Photobiologic Responses pp 335–368 Edited by Fitzpatrick T. B., Pathak M. A., Harber L. C., Seiji M., Kukita A. Tokyo: University of Tokyo Press;
    [Google Scholar]
  26. Reed L.J., Muench H. A. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  27. Scott B. R., Pathak M. A., Mohn G. R. 1976; Molecular and genetic basis of furocoumarin reactions. Mutation Research 39:29–74
    [Google Scholar]
  28. Shen C.-K. J., Hearst J. E. 1976; Psoralen-crosslinked secondary structure map of single-stranded virus DNA. Proceedings of the National Academy of Sciences of the United States of America 73:2649–2653
    [Google Scholar]
  29. Spikes J. D., Livingston R. 1969; The molecular biology of photodynamic action: sensitized photo-autoxidations in biological systems. In Advances in Radiation Biology vol 3 pp 29–121 Edited by Augenstein L. G. Mason R., Zelle M. New York: Academic Press;
    [Google Scholar]
  30. V St., Whitelock O. 1960; Inactivation of viruses. Annals of the New York Academy of Sciences 83:513–760
    [Google Scholar]
  31. Wiesehahn G. P., Hyde J. E., Hearst J. E. 1977; The photoaddition of trimethylpsoralen to Drosophilia melanogaster nuclei: a probe for chromatin substructure. Biochemistry 16:925–932
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-2-345
Loading
/content/journal/jgv/10.1099/0022-1317-40-2-345
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error