1887

Abstract

sp. strain TWAR is an important cause of acute respiratory disease in humans. To determine the genetic relatedness of the TWAR organism to and , solution hybridization followed by SI nuclease assays were performed, and guanine-plus-cytosine (G+C) contents were determined. Deoxyribonucleic acid (DNA) preparations from three TWAR isolates were radioactively labeled and hybridized against DNAs from three serovars of and five strains of . Reciprocal experiments were also done by using radioactively labeled and DNAs against TWAR DNA. In all cases, the degree of DNA relatedness between TWAR and the two species was 10% or less. Within the group of TWAR isolates, there was 94% or greater sequence similarity. The G+C values for TWAR were intermediate between the G+C values for and C. psittaci. The degrees of DNA relatedness among the strains within the species ranged from 20 to 100%.

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1988-07-01
2024-04-24
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