1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 21, Issue 4

Deoxyribonucleic Acid Homology Relationships Among Species of the Genus Free

Abstract

Genetic relatedness among 179 strains representing 13 named species and several unnamed taxa of the genus from three main habitats, i.e., (i) feces of man, (ii) feces of various other animals, and (iii) the bovine rumen, was assessed by means of deoxyribonucleic acid (DNA)—DNA hybridization by using a filter-paper technique in competition experiments. Assignment of these strains to the genus was based on the production of lactic and acetic acids as chief products from glucose, lack of gas production, fructose-6-phosphate phosphoketolase activity, and on morphology. About 180 DNA competitors were tested with 23 reference systems. Several genetically distinct groups were recognized. Little or no DNA homology was demonstrated between some of the groups, suggesting large evolutionary divergence in this genus. , and form one of these groups; the ecological significance of this relatedness was discussed. The DNA of the following pairs are homologous: (i) and , (ii) and , and (iii) and . Within a number of strains assigned to , many of which were isolated from waste waters, a large genetic heterogeneity was demonstrated: in addition to , at least three unrelated groups were recognized and are provisionally referred to as “,” and “.” Since these groups are not related genetically to any species of the genus and are phenotypically distinct, they may represent new species. The validity of the species , and was confirmed at the genetic level.

  • Published Online:
© American Society for Microbiology, 1971
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-21-4-276
1971-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/21/4/ijs-21-4-276.html?itemId=/content/journal/ijsem/10.1099/00207713-21-4-276&mimeType=html&fmt=ahah

References

  1. Beerens H., Gérard A., Guillaume J. 1957; Étude de 30 souches de Bifidobacterium bifidum (Lactobacillus bifidus). Caractérisation d’une variété buccale. Comparaison avec les souches d’origine fécale. Ann. Inst. Pasteur Lille 9:77–85
     [Google Scholar]
  2. Citarella R. V., Colwell R. R. 1970; Poly-phasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. J. Bacteriol. 104:434–442
     [Google Scholar]
  3. Crociani F., Scardovi V., Trovatelli L. D. 1970; Mannitol fermenting bifids from rumen and their DNA homology relationships. Ann. Microbiol. 20:99–106
     [Google Scholar]
  4. Dehnert J. 1957; Untersuchung über die grampositive Stuhlflora des Brustmilchkindes. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I. Orig. 169:66–79
     [Google Scholar]
  5. De Ley J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek J. Microbiol. Serol. 36:461–474
     [Google Scholar]
  6. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23:641–646
     [Google Scholar]
  7. de Vries W., Gerbrandy S. J., Stouthamer A. H. 1967; Carbohydrate metabolism in Bifidobacterium bifidum. Biochim. Biophys. Acta 136:415–425
     [Google Scholar]
  8. de Vries W., Stouthamer A. H. 1969; Factors determining the degree of anaerobiosis of Bifidobacterium strains. Arch. Mikrobiol. 65:275–287
     [Google Scholar]
  9. Exterkate F. A., Veerkamp J. H. 1969; Biochemical changes in Bifidobacterium bifidumvar. pennsylvanicus after cell wall inhibition. I. Composition of lipids. Biochim. Biophys. Acta (Amst.) 175:65–77
     [Google Scholar]
  10. Gibson T., Abd-el-Malek Y. 1945; The formation of carbon dioxide by lactic acid bacteria and Bacillus licheniformis and a cultural method of detecting the process. J. Dairy Res. 14:35–44
     [Google Scholar]
  11. Gyllenberg H., Carlberg G. 1958; The dominance of a specific nutritional type of Lactobacillus bifidus in breast-fed infants. Acta Pathol. Microbiol. Scand. 42:380–384
     [Google Scholar]
  12. Haenel H., Müller-Beuthow W., Grüfte F. K. 1970; Zur fäkalen Mikroökologie des Säuglings in Abhängigkeit von der Ernährung: Zusammensetzung der Mikroflora sowie vorkommen der Lactobacillus bifidus-Typen. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I. Orig. 215:333–347
     [Google Scholar]
  13. Heberlein G. T., De Ley J., Tijtgat R. 1967; Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium.. J. Bacteriol. 94:116–124
     [Google Scholar]
  14. Hoyer B. H., McCarthy B. J., Bolton E. T. 1964; A molecular approach in the systematics of higher organisms. Science (London) 144:959–967
     [Google Scholar]
  15. Johnson J. L., Ordal E. J. 1968; Deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. J. Bacteriol. 95:893–900
     [Google Scholar]
  16. Kandier O. 1970; Amino acid sequence of the murein and taxonomy of the genera Lactobacillus, Bifidobacterium, Leuconostoc, and Pediococcus.. Int. J. Syst. Bacteriol. 20:491–507
     [Google Scholar]
  17. Komagata K., Yamada K., Ogawa H. 1969; Taxonomic studies on coryneform bacteria. I. Division of bacterial cells. J. Gen. Appl. Microbiol. 15:243–259
     [Google Scholar]
  18. Lerche M., Reuter G. 1961; Isolierung und Differenzierung anaerober Lactobacilleae aus dem Darm erwachsener Menschen (Beitrag zum Lactobacillus bifidus-Problem).. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I Orig. 182:324–356
     [Google Scholar]
  19. Liebscher S. 1964; Differenzierung von Bifidusbakterien aus Säuglings und Erwachsenenstuhl. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I Orig. 193:60–70
     [Google Scholar]
  20. McCarthy B. J. 1967; Arrangement of base sequences in deoxyribonucleic acid. Bacteriol. Rev. 31:215–229
     [Google Scholar]
  21. McCarthy B. J., Bolton E. T. 1963; An approach to the measurement of genetic relatedness among organisms. Proc. Nat. Acad. Sei. U.S.A. 50:156–164
     [Google Scholar]
  22. Malyoth G., Bauer A. 1950; Beobachtungen am Bacterium bifidum.. Z. Kinderheilk. 68:358–367
     [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
     [Google Scholar]
  24. Marmur J., Doty P. 1961; Thermal renaturation of deoxyribonucleic acids. J. Mol. Biol. 3:585–594
     [Google Scholar]
  25. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
     [Google Scholar]
  26. Matteuzzi D., Crociani F., Zani G., Trovatelli L. D. 1971; Bifidobacterium suis n.sp.: a new species of the genus Bifidobacterium isolated from pig feces. Z. Allg. Mikrobiol. 11:387–395
     [Google Scholar]
  27. Mitsuoka T. 1969; Vergleichende Untersuchungen über die Bifidobakterien aus dem Verdauungstrakt von Menschen und Tieren. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I Orig. 210:52–64
     [Google Scholar]
  28. Mitsuoka T., Sega T., Yamamoto S. 1965; Eine verbesserte Methodik der qualitativen und quantitativen Analyse der Darmflora von Menschen und Tieren. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I Orig. 195:455–469
     [Google Scholar]
  29. Müller H., Pech H. 1967; Bifidusbakterien bei der Frau. Arch. Gynäkol. 205:39–58
     [Google Scholar]
  30. Orla-Jensen S. 1924; La classification des bactéries lactiques. Lait 4:468–474
     [Google Scholar]
  31. Orla-Jensen S., Orla-Jensen A. D., Winter O. 1936; Bacterium bifidum und Thermobacterium intestinale.. Zentralbl. Bakteriol. Parasitenk. Infectionskr. Hyg. Abt. II 93:321–343
     [Google Scholar]
  32. Petuely F., Lindner G. 1965; Kritische Untersuchung über die Darmflora. Ill Mitt.: Bewertung quantitativer Züchtungsmethoden. Die Darmflora des Brustkindes. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I Orig. 195:347–384
     [Google Scholar]
  33. Pine L. 1970; Classification and phylogenetic relationship of microaerophilic actinomycètes. Int. J. Syst. Bacteriol. 20:445–474
     [Google Scholar]
  34. Prévôt A. R. 1938; Etudes de systématique bactérienne. III. Invalidité du genre BacteroidesCastellani et Chalmers; démembrement et reclassification. Ann. Inst. Pasteur 60:285–307
     [Google Scholar]
  35. Prévôt A. R. 1970; Importance of the biochemical composition of the cell wall as a taxonomic characteristic. Int. J. Syst. Bacteriol. 20:539–540
     [Google Scholar]
  36. Reuter G. 1963-64; Vergleichende Untersuchungen über die Bifidu s-Flora im Sauglings- und Erwachsenenstuhl. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. I Orig. 191:486–507
     [Google Scholar]
  37. Reuter G. 1971; Designation of type strains for Bifidobacterium species. Int. J. Syst. Bacteriol. 21:273–275
     [Google Scholar]
  38. Scardovi V., Sgorbati B., Zani G. 1971; Starch gel electrophoresis of fructose-6-phosphate phosphoketolase in the genus Bifidobacterium.. J. Bacteriol. 106:1036–1039
     [Google Scholar]
  39. Scardovi V., Trovatelli L. D. 1965; The fructose-6-phosphate shunt as peculiar pattern of hexose degradation in the genus Bifidobacterium. Ann. Microbiol. 15:19–29
     [Google Scholar]
  40. Scardovi V., Trovatelli L. D. 1969; New species of bifid bacteria from Apis mellifica L. and Apis indica F. A contribution to the taxonomy and biochemistry of the genus Bifidobacterium.. Zentralbl. Bakteriol. Parasitenk. Infektionskr. Hyg. Abt. II 123:64–88
     [Google Scholar]
  41. Scardovi V., Trovatelli L. D., Crociani F., Sgorbati B. 1969; Bifid bacteria in bovine rumen. New species of the genus Bifidobacterium: B. globosum n. sp. and B. ruminale n. sp. Arch. Mikrobiol. 68:278–294
     [Google Scholar]
  42. Scardovi V., Zani G., Trovatelli L. D. 1970; Deoxyribonucleic acid homology among the species of the genus Bifidobacterium isolated from animals. Arch. Mikrobiol. 72:318–325
     [Google Scholar]
  43. Schramm M., Klybas V., Racker F. 1958; Phosphorolytic cleavage of fructose-6-phosphate phosphoketolase from Acetobacter xylinum.. J. Biol. Chern. 233:1283–1288
     [Google Scholar]
  44. Seeliger H. P. R„, Werner H. 1963; Recherches qualitatives et quantitatives sur la flore intestinale de l’homme. Ann. Inst. Pasteur 105:911–936
     [Google Scholar]
  45. Sgorbati B., Zani G., Trovatelli L. D., Scardovi V. 1970; Gluconate dissimilation by the bifid bacteria of the honey bee. Ann. Microbiol. 20:57–64
     [Google Scholar]
  46. Werner H., Seeliger H. P. R. 1963; Kulturelle Untersuchungen über die Vaginalflora unter besonderer Berücksichtigung der Bifidusbakterien. Pathol. Microbiol. 26:53–73
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-21-4-276
Loading
Deoxyribonucleic Acid Homology Relationships Among Species of the Genus Bifidobacterium
Int J Syst Evol Microbiol 21, 276 (1971); https://doi.org/10.1099/00207713-21-4-276
/content/journal/ijsem/10.1099/00207713-21-4-276
/content/journal/ijsem/10.1099/00207713-21-4-276
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error