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Volume 72, Issue 10

sp. nov. a novel phytopathogenic bacterium isolated from black spots of tobacco Open Access

Abstract

Three Gram-stain-negative, motile, with amphilophotrichous flagella, and rod-shaped bacteria (LJ1, LJ2 and LJ3) were isolated from lower leaves with black spots on flue-cured tobacco in Yunnan, PR China. The results of phylogenetic analysis based on 16S rRNA gene sequences indicate that all the strains from tobacco were closely related to the type strains of the group within the lineage and LJ2 has the highest sequence identities with DSM 50259 (99.92 %), Pc19-1 (99.67 %) and F51 (98.94 %) . The 16S rRNA gene sequence identities between LJ2 and other members of the genus were below 98.50%. The average nucleotide identity by (ANIb) values between LJ2 and DSM 50259, Pc19-1 and F51 were less than 95 %, and the DNA–DNA hybridization (DDH) values (yielded by formula 2) were less than 70 %. The major fatty acids were Cω7 and/or Cω6 (summed feature 3), C and Cω7 and/or Cω6 (summed feature 8). The polar lipids profile of LJ2 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids and one unidentified glycolipid. The predominant respiratory quinone was Q-9. The DNA G+C content of LJ2 was 58.4 mol%. On the basis of these data, we concluded that LJ2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is LJ2 (=CCTCC AB 2021465=GDMCC 1.2884=JCM 35177).

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  • Accepted:
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Keyword(s): black spot of tobacco , plant-pathogenic bacterium , Pseudomonas and Pseudomonas fluorescens lineage
Funding
This study was supported by the:
  • Yunnan Provincial Tobacco Monopoly Bureau; National Natural Science Foundation of China (Award 2020530000241013, 2018530000241006, 2019530000241007 and 2020530000241012; 32260702)
    • Principle Award Recipient: LUCAN-HUA
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-10-25
2024-04-28
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Pseudomonas lijiangensis sp. nov., a novel phytopathogenic bacterium isolated from black spots of tobacco
Int J Syst Evol Microbiol 72, 005591 (2022); https://doi.org/10.1099/ijsem.0.005591
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