SCIENTIFIC ARTICLESEffectiveness of Intracanal Irrigants and Medications against the Yeast Candida albicans
Section snippets
Experimental Design
A dilution technique was used to expose a standardized inoculum of C. albicans to various concentrations of water-soluble medications. The medications tested were sodium hypochlorite (NaOCl), hydrogen peroxide (H2O2), calcium hydroxide (Ca(OH)2), and chlorhexidine digluconate. Negative controls, consisting of culture media with no test material added, were used to ensure that the yeast was viable in each experiment. Amphotericin B was used as a positive control to ensure that the yeast was
Controls
All negative controls, i.e. culture tubes in which no medication was placed, were positive for C. albicans growth. The mean OD530 ± SE for negative controls using RPMI growth medium was 0.640 ± 0.058. Chlorhexidine digluconate precipitated in RPMI; using half-strength RPMI to test the medication obviated this problem. The mean OD530 ± SE for negative controls using half-strength RPMI was 0.391 ± 0.015.
The mean OD530 of C. albicans growing in the positive, amphotericin B, controls was observed.
DISCUSSION
This in vitro study examined commonly used intracanal medications and irrigants and determined the MIC of each that is required to kill a standardized population of C. albicans. All negative controls were positive for fungal growth, indicating that the experimental protocol did allow for the growth of C. albicans. The positive controls showed complete inhibition of C. albicans at concentrations of 0.250 μg amphotericin B/ml and stronger. These results agree with published ranges of MICs for
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This research was supported by a grant from the Southern Illinois University School of Dental Medicine Research Committee.