Scientific ArticlesCD45/Isotypes Expression in the Immune Cells of Human Periapical Lesions
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MATERIALS AND METHODS
The periapical granulation tissue samples (n = 25) were obtained from patients undergoing surgical treatment. Tissue samples were immediately frozen in liquid nitrogen and kept at −70°C until being sectioned. Indirect immunoperoxidase procedure used in the present study is described in detail previously (9). Sections were fixed in acetone for 10 min and air-dried for at least 30 min. Sections were then incubated with one of the primary antibodies listed in Table 1 for 60 min. Dilutions of the
RESULTS
The periapical granulation tissue samples examined comprised various types of blood vessels, including high endothelial venules, connective tissue elements, and a number of leukocytes that were predominantly mononuclear cells and a few granulocytes. In some of the samples, a part of the tissue was lined by a stratified squamous epithelium. We did not observe any cysts or scar tissue in any of the samples examined, therefore all 25 cases were considered as periapical granulomas.
Examinations with
DISCUSSION
Long-lasting dental pulp inflammations lead to lesions at the periapical regions of the involved teeth. The majority of these reactions are represented by a granulomatous tissue, although cyst and scar formations in some cases are also evident (1, 2, 3, 4, 5). The granulomatous tissue is rich in granulocytes at acute stages, whereas mononuclear cells dominate at chronic stages of inflammation (1, 2, 3, 4, 5). Several investigators examined the nature of immune cells in periapical lesions by
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Cited by (3)
Expression of macrophage inflammatory protein 3α in human inflamed dental pulp tissue
2005, Journal of Endodontics
We acknowledge M. Oğuz Güç, M.D., Ph.D., for critical reading of this manuscript.