Elsevier

Journal of Endodontics

Volume 26, Issue 3, March 2000, Pages 166-168
Journal of Endodontics

Scientific Articles
CD45/Isotypes Expression in the Immune Cells of Human Periapical Lesions

https://doi.org/10.1097/00004770-200003000-00009Get rights and content

Expression of some leukocyte antigens (including CD45) and its isoforms (CD2, CD4, CD5, CD6, CD7, and CD8) was examined in the human periapical granulation tissue samples in the present study. The majority of the infiltrating cells expressed heavy molecular-weight isoforms of the CD45 antigen. Expression of CD2, CD5, CD6, and CD7 antigens was also detected, implying significant roles for these antigens in the immune reaction taking place in periapical lesions. This suggests that the immune response taking place at the periapical region is predominantly cellular and the humoral responses to antigenic challenge are conducted mainly by regional lymph nodes.

Section snippets

MATERIALS AND METHODS

The periapical granulation tissue samples (n = 25) were obtained from patients undergoing surgical treatment. Tissue samples were immediately frozen in liquid nitrogen and kept at −70°C until being sectioned. Indirect immunoperoxidase procedure used in the present study is described in detail previously (9). Sections were fixed in acetone for 10 min and air-dried for at least 30 min. Sections were then incubated with one of the primary antibodies listed in Table 1 for 60 min. Dilutions of the

RESULTS

The periapical granulation tissue samples examined comprised various types of blood vessels, including high endothelial venules, connective tissue elements, and a number of leukocytes that were predominantly mononuclear cells and a few granulocytes. In some of the samples, a part of the tissue was lined by a stratified squamous epithelium. We did not observe any cysts or scar tissue in any of the samples examined, therefore all 25 cases were considered as periapical granulomas.

Examinations with

DISCUSSION

Long-lasting dental pulp inflammations lead to lesions at the periapical regions of the involved teeth. The majority of these reactions are represented by a granulomatous tissue, although cyst and scar formations in some cases are also evident (1, 2, 3, 4, 5). The granulomatous tissue is rich in granulocytes at acute stages, whereas mononuclear cells dominate at chronic stages of inflammation (1, 2, 3, 4, 5). Several investigators examined the nature of immune cells in periapical lesions by

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Cited by (3)

We acknowledge M. Oğuz Güç, M.D., Ph.D., for critical reading of this manuscript.

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