Elsevier

Journal of Endodontics

Volume 26, Issue 2, February 2000, Pages 81-84
Journal of Endodontics

Scientific Article
Endothelial Cell and Stromal Antigens in Human Periapical Granulation Tissue

https://doi.org/10.1097/00004770-200002000-00005Get rights and content

Periapical granulation tissue consists of vasculature of varying sizes and types, infiltrating cells, and other stromal elements. We examined the differential expression of endothelial and stroma antigens in this tissue to determine their tissue distribution in order to obtain hints on their functions. Some of the antigens examined were present only in the endothelial lining of vasculature, including high endothelial venules (e.g. CD31 and CD105), whereas others were more widely expressed by both vascular and stromal elements (e.g. CD29, CD63, CD44, and CD151). Immunohistochemical analysis using monoclonal antibodies specific to certain tissue compartments revealed the tissue architecture more precisely and the expression of certain antigens in the tissue suggested special roles for these antigens. Tissue distribution of CD63, CD143, CD147, and CD151 in periapical granulation tissue is first reported in the present study.

Section snippets

MATERIALS AND METHODS

The periapical granulation tissue samples (n = 25) were obtained from patients undergoing surgical treatment. The samples were immediately frozen in liquid nitrogen and kept at −70°C until being sectioned. The indirect immunoperoxidase staining procedure used in the study was described in detail previously (14). Sections were fixed in acetone for 10 min and air-dried for at least 30 min. Sections were then incubated with the primary antibodies listed in Table 1 for 60 min. Dilutions of the

RESULTS

We examined periapical granulation tissue samples to determine the vascular pattern and stromal elements of the tissue by using markers specific for the endothelial cells and stromal elements. The granulation tissue consisted of various types of blood vessels, including high endothelial venules, connective tissue elements, and a number of leukocytes, predominantly mononuclear cells and a few granulocytes. High endothelial venules are special postcopillary venules through which lymphocyte

DISCUSSION

A variety of cell surface antigens were examined to determine their expression patterns and intensities in the periapical granulation tissue samples obtained from patients undergoing surgical treatment with special reference to those expressed by the vascular endothelium. CD29, the common β1 chain for VLA integrins is an antigen broadly expressed by a variety of cell types, including vasculature and connective tissue fibroblasts (15, 16). We also detected a similar reactivity pattern of this

References (20)

There are more references available in the full text version of this article.

Cited by (6)

  • CD44 Presence in Inflamed Pulp Tissue

    2007, Journal of Endodontics
    Citation Excerpt :

    Even though several in vitro studies have raised awareness of how CD44 is involved in the induction of proinflammatory events, it is still uncertain whether such mechanisms are present in the living being. Hyaluronate receptor CD44 was expressed mainly by the epithelium and connective tissue elements, including a majority of the mononuclear infiltrating cells (41). During the inflammatory process, matrix protein degeneration is produced in the pulp, requiring the activity of several endopeptidases, proteolytic enzymes that are essential for the degradation of the extracellular matrix.

View full text