Scientific ArticleEndothelial Cell and Stromal Antigens in Human Periapical Granulation Tissue
Section snippets
MATERIALS AND METHODS
The periapical granulation tissue samples (n = 25) were obtained from patients undergoing surgical treatment. The samples were immediately frozen in liquid nitrogen and kept at −70°C until being sectioned. The indirect immunoperoxidase staining procedure used in the study was described in detail previously (14). Sections were fixed in acetone for 10 min and air-dried for at least 30 min. Sections were then incubated with the primary antibodies listed in Table 1 for 60 min. Dilutions of the
RESULTS
We examined periapical granulation tissue samples to determine the vascular pattern and stromal elements of the tissue by using markers specific for the endothelial cells and stromal elements. The granulation tissue consisted of various types of blood vessels, including high endothelial venules, connective tissue elements, and a number of leukocytes, predominantly mononuclear cells and a few granulocytes. High endothelial venules are special postcopillary venules through which lymphocyte
DISCUSSION
A variety of cell surface antigens were examined to determine their expression patterns and intensities in the periapical granulation tissue samples obtained from patients undergoing surgical treatment with special reference to those expressed by the vascular endothelium. CD29, the common β1 chain for VLA integrins is an antigen broadly expressed by a variety of cell types, including vasculature and connective tissue fibroblasts (15, 16). We also detected a similar reactivity pattern of this
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