Abstract
A deoxynivalenol (DON) monoclonal antibody (DON-Mab) was labeled with gold nanospherical (AuNSs) with a particle size of 45±1.9 nm. The DON Immunochromatographic test card (DON-GICT) for detecting DON in food and feed was developed. The sample does not require special handling. The detection process takes about 20-25 minutes. In conjunction with the nano gold reader, the detection range of the actual sample by DON-GICT is 6.25-6250 μg/kg. DON standards with a final concentration of 500μg/kg were added to 5 grains and 1 feed sample, respectively, and the recovery was 98.3%-127.0%, and the coefficient of variation was 0.9%-4.7%. Twenty-three feed samples and 11 food samples were tested by DON-ELISA and DON-GICT, respectively, and the results were compared. The linear fitting correlation coefficient R2 = 0.9845. It shows that DON-GICT has good accuracy, precision and wide detection range, which can provide efficient and accurate detection method for detecting DON pollution in food and feed.
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