Abstract
The mechanical and optical components are described of an integrating microspectrophotometer, which can either measure absorption spectra of specimens as small as 1 μm in diameter, or integrate the absorbances measured at one wavelength from any irregular specimen. Facilities are provided for making an outline drawing of any part of the specimen, which can then control the selection of areas to be integrated.
The electronic circuits include a high-speed electronic servo for maintaining the photomultiplier signal constant, logarithmic conversion and integrating elements. Various tests of the instrument are described.