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Use of Spoligotype Analysis to Detect Laboratory Cross-Contamination

Published online by Cambridge University Press:  02 January 2015

Beth Nivin ,
Jeffrey Driscoll ,
Tom Glaser ,
Pablo Bifani  and
Sonal Munsiff
Beth Nivin*
Affiliation:
New York City Department of Health, Tuberculosis Control Program, New York City, New York
Jeffrey Driscoll
Affiliation:
New York State Department of Health, Wadsworth Center, New York City, New York
Tom Glaser
Affiliation:
New York City Department of Health, Tuberculosis Control Program, New York City, New York
Pablo Bifani
Affiliation:
Public Health Research Institute, New York City, New York
Sonal Munsiff
Affiliation:
New York City Department of Health, Tuberculosis Control Program, New York City, New York
*
Tuberculosis Control Program, New York City Department of Health, 225 Broadway, 22nd Floor, New York City, NY 10007
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Abstract

Spoligotype analysis identified false-positive isolates of Mycobacterium tuberculosis caused by laboratory cross-contamination. Spoligotyping is faster, is less expensive than DNA fingerprinting, and can be used with a variety of media. Patients were reevaluated and had medications discontinued as a result of this investigation. Months of unnecessary patient follow-up and treatment were avoided.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 21 , Issue 8 , August 2000 , pp. 525 - 527
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2000

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References

1.Braden, CR, Templeton, GL, Stead, WS, Bates, JH, Cave, MD, Valway, SE. Retrospective detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures with use of DNA fingerprint analysis. Clin Infect Dis 1997;24:35–40.CrossRefGoogle ScholarPubMed
2.Frieden, TR, Woodley, CL, Crawford, JT, Lew, D, Dooley, SM. The molecular epidemiology of tuberculosis in New York City: the importance of nosocomial transmission and laboratory error. Tuber Lung Dis 1996;77:407–413.Google Scholar
3.Wurtz, R, Demairais, P, Trainor, W, McAuley, J, Kocka, F, Mosher, L, et al. Specimen contamination in mycobacteriology laboratory detected by pseudo-outbreak of multidrug-resistant tuberculosis: analysis by routine epidemiology and confirmation by molecular technique. J Clin Microbiol 1996;34:1017–1019.Google Scholar
4.Burman, WJ, Stone, BL, Reves, RR, Wilson, ML, Yang, Z, El-Hajj, H, et al. The incidence of false-positive cultures for Mycobacterium tuberculosis. Am J Respir Crit Care Med 1997;155:321–325.CrossRefGoogle ScholarPubMed
5.Nivin, B, Fujiwara, PI, Hannifin, J, Kreiswirth, BN. Cross-contamination with Mycobacterium tuberculosis: an epidemiological and laboratory investigation. Infect Control Hosp Epidemiol 1998;19:500–503.Google Scholar
6.Nivin, B, Kaye, K, Munsiff, SS. Detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures. Clin Infect Dis 1997;25:943.Google Scholar
7.Goual, M, Saunders, NA, van Embden, JD, Young, DB, Shaw, RJ. Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism. J Clin Microbiol 1997;35:647–651.Google Scholar
8.Kamerbeek, J, Schouls, L, Kolk, A, van Agterveld, K, van Soolingen, D, Kuijper, S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 1998;35:907–914.Google Scholar
9.Driscoll, JR, McGarry, MA, Taber, HW. DNA typing of a nonviable culture of Mycobacterium tuberculosis in a homeless shelter outbreak. J Clin Microbiol 1999;37:274–275.CrossRefGoogle Scholar
10.van Embden, JD, Cave, MD, Crawford, JT, Dale, JW, Eisenbach, KD, Gicquel, B, et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 1993;31:406–409.CrossRefGoogle ScholarPubMed