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Evidence of Nosocomial Stenotrophomonas Maltophilia Cross-Infection in a Neonatology Unit Analyzed by Three Molecular Typing Methods

Published online by Cambridge University Press:  02 January 2015

Darío García de Viedma*
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Mercedes Marín
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Emilia Cercenado
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Roberto Alonso
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Marta Rodríguez-Créixems
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Emilio Bouza
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
*
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, ” C/Dr. Esquerdo, 46. 28007 Madrid, Spain

Abstract

Objective:

To characterize the epidemiological relationships among Stenotrophomonas maltophilia isolates in the neonatology unit of our institution over a 4-month period in which an increased number of isolates was observed.

Setting:

The neonatology ward in a 2,000-bed university hospital in Madrid, Spain.

Design:

A retrospective molecular epidemiological analysis using three different typing methods, arbitrarily primed polymerase chain reaction (PCR), pulsed-field gel electrophoresis, and enterobacterial repetitive intergenic consensus-PCR, was performed with 11 isolates obtained from seven neonates over a 4-month period. Presumed unrelated isolates also were included as controls. A similarity dendrogram was obtained, to analyze the genetic relatedness among the isolates.

Results:

All isolates from the neonates, except one, showed a remarkably high homology among their typing patterns for the three methods assayed and clustered in the relatedness dendrogram at 96% similarity. The unrelated strains selected as controls were unclustered. The index case was considered to be a newborn who had an S maltophilia isolate from a culture drawn on the day of admission to the neonatology unit and which was included in the clustered similarity group.

Conclusions:

Such a high genetic similarity among the isolates, together with the presence of an index case who had been colonized or infected by S maltophilia before arrival at our institution, constitutes the first evidence of nosocomial cross-transmission of this microorganism.

Type
Original Articles
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1999

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