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Growth inhibition of human endothelial cells by the phyto-oestrogen biochanin A, a metabolite of genistein

Published online by Cambridge University Press:  09 March 2007

Chingwen Ying ,
Jih-Tay Hsu  and
Show-Chi Shieh
Chingwen Ying*
Affiliation:
Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China
Jih-Tay Hsu
Affiliation:
Department of Animal Sciences, National Taiwan University, Taipei, Taiwan, Republic of China
Show-Chi Shieh
Affiliation:
Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China
*
*Corresponding author: Dr C. Ying, fax +886-2-2883-1193, email cying@mail.scu.edu.tw
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Abstract

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This study examined the growth regulatory effects of a phyto-oestrogen, biochanin A, on a transformed human endothelial cell line ECV304 in vitro. Biochanin A was found to inhibit cell proliferation in a dose-dependent fashion and this effect was influenced by the concentration of serum present in the culture medium. In the absence of serum, the calculated IC50 of biochanin A was 0.18 ± 0.1 μM COMPARED TO AN IC50 OF 35±5 Μm at 10 % serum. At low cell density, the growth inhibitory effects of biochanin A were more evident than at high cell density. Co-administration of a synthetic oestrogen diethylstilboestrol with biochanin A did not suppress the growth regulatory effects of biochanin A treatment. We conclude that biochanin A inhibits the cell proliferation of human endothelial cells at concentrations that are physiologically achievable in vivo and that this effect may play an important role in the cancer-preventing activity of the phyto-oestrogens.

Keywords

Phyto-oestrogenEndothelial cellsCell proliferation
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 5 , May 2001 , pp. 615 - 620
Copyright
Copyright © The Nutrition Society 2001

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