Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-25T04:13:54.277Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic variation of Marchalina hellenica (Hemiptera: Margarodidae) sampled from different hosts and localities in Greece

Published online by Cambridge University Press:  09 March 2007

J.T. Margaritopoulos ,
N. Bacandritsos ,
A.N. Pekas ,
C. Stamatis ,
Z. Mamuris  and
J.A. Tsitsipis
J.T. Margaritopoulos*
Affiliation:
Department of Agriculture, Crop Production and Agricultural Environment, University of Thessaly, Fytokou Str.,38446 Nea Ionia, Magnesia, Greece
N. Bacandritsos
Affiliation:
Institute of Veterinary Research of Athens, Neapoleos 25, Agia Paraskevi, 15310 Athens, Greece
A.N. Pekas
Affiliation:
Department of Agriculture, Crop Production and Agricultural Environment, University of Thessaly, Fytokou Str.,38446 Nea Ionia, Magnesia, Greece
C. Stamatis
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26, Larissa 41221, Greece
Z. Mamuris
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26, Larissa 41221, Greece
J.A. Tsitsipis
Affiliation:
Department of Agriculture, Crop Production and Agricultural Environment, University of Thessaly, Fytokou Str.,38446 Nea Ionia, Magnesia, Greece
*
*Fax: +30 24210 93286 E-mail: jmarg@uth.gr
Get access Rights & Permissions [Opens in a new window]

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to 120 individuals of Marchalina hellenica (Gennadius) representing six populations collected in northern, central and southern mainland Greece. One population was sampled on one species of fir tree and the others on two species of pine trees. Four random decamer primers were used to evaluate genetic variation among the populations examined. The results revealed intra- and interpopulation polymorphism both related to host type and region of origin. Phylogenetic analysis based on genetic distances estimated by the RAPD frequencies revealed an important genetic differentiation in samples collected on fir trees in southern Greece and to a lesser extent in samples from pine trees in central and northern Greece. Furthermore, considerable subdivision and restricted gene flow among the populations examined were observed. The results are discussed in relation to the biology and geographical distribution of M. hellenica in Greece.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 93 , Issue 5 , September 2003 , pp. 447 - 453
Copyright
Copyright © Cambridge University Press 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bacandritsos, N. (1998) Control of mite diseases of honeybees without use of pesticides. PhD thesis, Agricultural University of Athens (in Greek).Google Scholar
Bacandritsos, N. (2002) A scientific note on the first successful establishment of the monophlebine coccid Marchalina hellenica (Coccoidea, Margarodidae) on the fir tree (Abies cephalonica). Apidologie 33, 353–354.CrossRefGoogle Scholar
Black, W.C. (1993) PCR with arbitrary primers: approach with care. Insect Molecular Biology 2, 1–6.CrossRefGoogle ScholarPubMed
Black, W.C. IV., DuTeau, N.M., Puterka, G.J., Nechols, J.R. & Pettorini, J.M. (1992) Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids. Bulletin of Entomological Research 82, 151–159.Google Scholar
Chatfield, C. & Collins, A.J. (1980) Introduction to multivariate analysis. London, Chapman & Hall.CrossRefGoogle Scholar
Erlinghagen, F. (2001) Portrait of an insect: Marchallina hellenica Genn. (Sternorryhncha: Coccina: Margarodidae), important producer of honeydew in Greece. Apiacta 36, 131–137.Google Scholar
Fimiani, P. & Sollino, G. (1994) An exotic insect dangerous to the native plants of the island of Ischia. Informatore Agrario 50, 65–68.Google Scholar
Frankham, R. (1995 a) Inbreeding and extinction: a threshold effect. Conservation Biology 9, 792–799.CrossRefGoogle Scholar
Frankham, R. (1995 b) Conservation genetics. Annual Review of Genetics 29, 305–327.Google Scholar
Hadrys, H., Balick, M. & Scierwater, B. (1992) Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology 1, 55–63.Google Scholar
Holsinger, K.E., Paul, O.L. & Dirak, K.D. (2002) A Bayesian approach to inferring population structure from dominant markers. Molecular Ecology 11, 1157–1164.Google Scholar
Jones, J.S. (1980) Can genes choose habitats? Nature 286, 757–758.CrossRefGoogle ScholarPubMed
Kailidis, D.L. (1965) Monophlebus hellenicus (Marchalina hellenica) Gen. The melitogenic insect of the pine tree. Dasika Chronica 81/82, 1–15 (in Greek).Google Scholar
Kailidis, D.S. (1991) Forest entomology and zoology. 4th edn. Thessaloniki, Christodoulidis Press. (in Greek).Google Scholar
Lynch, M. & Milligan, B.G. (1994) Analysis of population genetic structure with RAPD markers. Molecular Ecology 3, 91–99.Google Scholar
Nei, M. (1972) Genetic distance between populations. American Naturalist 106, 283–232.CrossRefGoogle Scholar
Nikolopoulos, C. (1964) Sur la decouverte de l'inconnum jusqu'aujourd'hui individuale de l'espece. Marchalina hellenica. (Gennadius) (Hemiptera, Margarodidae, Coclostomidiinae). Ecole de Hautes Etudes Agronomique Athenes, Lab. Zool. Agr. Et Series, 1964, 3–15 (in Greek).Google Scholar
Nikolopoulos, C. (1965) Morphology and biology of the species. Marchalina hellenica. (Gennadius) (Hemiptera, Margarodidae, Coclostomidiinae). Ecole de Hautes Etudes Agronomique Athenes, Lab. Zool. Agr. Et Series, 1964, 3–31 (in Greek).Google Scholar
Pollini, A. (1998) Manuale di entomologia applicata. Bologna, Edagricole-Edizioni Agricole.Google Scholar
Santas, L.A. (1983) Insects producing honeydew exploited by bees in Greece. Apidologie 14, 93–103.Google Scholar
Sneath, P.H.A. & Sokal, R.R. (1973) Numerical taxonomy. San Francisco, W.H. Freeman & Co.Google Scholar
Sureyya, M. & Hovasse, R. (1930) Les ennemis des pins aux Iles Princes. Istanbul.Google Scholar
Spiegelhater, D.J., Best, N.G., Carlin, B.P. & van der Linde, A. (2002) Bayesian measures of model complexity and fit. Journal of the Royal Statistical Society Series B 64, 483–689.Google Scholar
Thrasyvoulou, A. & Manikis, I. (1995) Some physicochemical and microscopic characteristics of Greek unifloral honeys. Apidologie 26, 441–452.Google Scholar
Tremblay, E. (1995) Entomologia applicata. Volume 2. Napoli, Liguori Editore.Google Scholar
Wallner, W.E. (1987) Factors affecting insect population dynamics: differences between outbreak and non-outbreak species. Annual Review of Entomology 32, 317–340.Google Scholar
Waples, R. (1987) A multispecies approach to the analysis of gene flow in marine shore fishes. Evolution 41, 385–400.CrossRefGoogle Scholar
Williams, J.G.K., Hanafey, M.K., Rafalski, J.A. & Tingey, S.V. (1993) Genetic analysis using random amplified polymorphic DNA markers. Methods in Enzymology 218, 704–740.Google Scholar