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Classification of Brachyspira spp. isolated from Swedish dogs

Published online by Cambridge University Press:  28 February 2007

Claes Fellström ,
Bertil Pettersson ,
Ulla Zimmerman ,
Anders Gunnarsson  and
Ricardo Feinstein
Claes Fellström*
Affiliation:
Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Uppsala, Sweden
Bertil Pettersson
Affiliation:
Department of Biotechnology, The Royal Institute of Technology, Stockholm, Sweden
Ulla Zimmerman
Affiliation:
Department of Bacteriology, The National Veterinary Institute, Uppsala, Sweden
Anders Gunnarsson
Affiliation:
Department of Bacteriology, The National Veterinary Institute, Uppsala, Sweden
Ricardo Feinstein
Affiliation:
Department of Pathology, The National Veterinary Institute, Uppsala, Sweden
*
*Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7018, SE-750 07 Uppsala, Sweden. E-mail: Claes.Fellstrom@kirmed.slu.se
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Abstract

Brachyspira spp. were isolated from 21 of 32 sampled dogs (66%) in a colony of Swedish beagle dogs with a history of diarrhea and from 3 of 17 Swedish pet dogs (17%) with diarrhea. All Swedish isolates were weakly β-hemolytic and gave a negative indole reaction. Eighty-eight percent showed negative α-galactosidase and hippurate reactions, but a positive β-glucosidase reaction. Two isolates were hippurate positive with a negative β-glucosidase reaction. One additional German isolate diverged by showing a positive indole reaction in combination with a positive hippurate reaction. Sequencing of 16S rDNA indicated that the hippurate-positive isolates belonged to the species Brachyspira pilosicoli. Four representative isolates were examined using pulsed-field gel electrophoresis (PFGE) and compared with six reference strains and five porcine isolates of Brachyspira spp. The canine isolates clustered together in the PFGE analysis. Necropsy examination of a culture-positive B. pilosicoli colony-raised beagle dog revealed macro- and microscopical lesions of colitis with numerous spiral-shaped bacteria in the lumens of the crypts, in goblet cells and within the colonic epithelium.

Type
Research Article
Information
Animal Health Research Reviews , Volume 2 , Issue 1 , June 2001 , pp. 75 - 82
Copyright
Copyright © CAB International 2001

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