Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-04-30T11:44:45.083Z Has data issue: false hasContentIssue false
  • English
  • Français

Influence of iron supplementation frequency on absorption efficiency and mucosal ferritin in anaemic rats

Published online by Cambridge University Press:  09 March 2007

Paloma Benito ,
William House  and
Dennis Miller
Paloma Benito
Affiliation:
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
William House
Affiliation:
US Plant, Soil and Nutrition Laboratory, USDA-ARS, Ithaca, NY 14853, USA
Dennis Miller
Affiliation:
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

It is believed that frequent Fe doses decrease the efficiency of absorption as a consequence of the loading of intestinal mucosal cells with Fe from the previous supplemental dose. We examined this premise in thirty anaemic Sprague-Dawley rats given Fe supplements as FeSO4 in 1 g preparations of a 50:50 (w/w mixture of low-Fe diet and sucrose under one of the following regimens: one 3 mg Fe dose daily for 3d, four 0.75mg doses daily at 6h intervals for 3d, and one 9mg dose on day 1 followed by two placebo (low-Fe diet) doses on days 2 and 3. All groups were fed on two low-Fe meals daily (8.3 mg Fe/kg diet). After an overnight fast rats were dosed with 1 ml of an 59Fe-labelled ferric nitrilotriacetic acid solution (37 kBq 59Fe, 50 μg Fe) orally and killed 10 h later. Absorption of 59Fe was measured as the percentage of the 59Fe retained by the carcass without the gastrointestinal tract 10 h after dosing relative to the initial 59Fe dose. Haemoglobin-Fe gain, liver non-haem-Fe, and mucosal duodenal ferritin were determined after the 3 d supplementation period. Absorption of the test dose in rats supplemented once 3 d before assessment of Fe absorption was 2.6-fold greater than those supplemented with daily single doses and 1.9-fold greater than those supplemented with daily multiple doses. Our data indicate that both mucosal ferritin and liver Fe levels account for the higher absorption efficiency found in rats supplemented once to simulate intermittent regimens.

Keywords

IronFerritinMucosa
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 3 , September 1997 , pp. 469 - 477
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Bothwell, T. H. & Finch, C. A. (1962) Iron Metabolism. London: J. & A. Churchill Ltd.Google Scholar
Conrad, M. E., Parmley, R. T. & Osterloh, K. (1987) Small intestinal regulation of iron absorption in the rat. Journal of Laboratory and Clinical Medicine 110, 418–426.Google Scholar
DeMaeyer, E. (1989) Preventing and Controlling Iron Deficiency Anemia through Primary Health Care. Geneva: WHO.Google Scholar
DeMaeyer, E. & Adiels-Tegman, M. (1985) The prevalence of anemia in the world. World Health Statistics Quarterly 38, 302–316.Google Scholar
Hahn, P. F., Bale, W. F., Ross, J. F., Balfour, W. M. & Whipple, G. H. (1943) Radioactive iron absorption by the gastrointestinal tract: influence of anaemia, anoxia, and antecedent feeding. Journal of Experimental Medicine 78, 169–188.CrossRefGoogle ScholarPubMed
Hahn, P. F., Carothers, E. L., Darby, W. J., Martin, M., Sheppard, C. W., Cannon, R. O., Beam, A. S., Densen, P. M., Peterson, J. C. & McClellan, G. S. (1951) Iron metabolism in human pregnancy as studied by radioactive isotope, 59Fe. American Journal of Obstetrics and Gynecology 61, 477–486.Google Scholar
Hanks, J. H. & Wallace, R. E. (1949) Relation of oxygen and temperature in the preservation of tissues by refrigeration. Proceedings of the Society for Experimental Biology and Medicine 71, 196–200.CrossRefGoogle ScholarPubMed
Holt, P. R., Kotler, D. P. & Pascal, R. R. (1983) A simple method for determining epithelial cell turnover in small intestine: studies in young and aging rat gut. Gastroenterology 84, 69–74.Google Scholar
Mahoney, A. W. & Hendricks, D. G. (1976) Effect of dietary iron level on efficiency of converting food iron into haemoglobin by the anaemic rat. Nutrition and Metabolism 20, 222–227.CrossRefGoogle ScholarPubMed
Miller, D. K., Smith, V. L., Kanner, J., Miller, D. D. & Lawless, H. T. (1994) Lipid oxidation and warmed-over aroma in cooked ground pork from swine fed increasing levels of iron. Journal of Food Science 59, 751–756.Google Scholar
Minitab, (1994) Minitab for Windows, Release 10. State College, PA: Minitab Inc.Google Scholar
Momtazi, S. & Herbert, V. (1973) Intestinal absorption using vibration-obtained individual small bowel epithelial cells of the rat: folate absorption. American Journal of Clinical Nutrition 26, 23–29.Google Scholar
Mudd, A. J. & Stranks, M. H. (1985). Mineral and trace element requirements. In Recent Developments in Pig Nutrition, pp. 124–138 [Cole, D.J. A. and Haresing, W., editors]. London: Butterworths.Google Scholar
National Committee for Clinical Laboratory Standards (1984) Reference Procedure for the Quantitative Determination of Haemoglobin in Blood; Approved Standard. Villanova, PA: National Committee for Clinical Laboratory Standards.Google Scholar
Norrby, A. (1974). Iron absorption studies in iron deficiency. Scandinavian Journal of Haematology 20, Suppl., 33–125.Google Scholar
Savin, M. A. & Cook, J. D. (1978) Iron transport by isolated rat intestinal mucosal cells. Gastroenterology 75, 688–694.CrossRefGoogle ScholarPubMed
Smith, J. A., Drysdale, J. W., Goldberg, A. & Munro, H. N. (1968) The effect of enteral and parenteral iron on ferritin synthesis in the intestinal mucosa of the rat. British Journal of Haematology 14, 79–86.CrossRefGoogle ScholarPubMed
Snedecor, G. W. & Cochran, W. G. (1989) Statistical Methods. Ames, IA: Iowa State University Press.Google Scholar
Torrance, J. D. & Bothwell, T. H. (1968) A simple technique for measuring storage iron concentrations in formalinized liver samples. South African Journal of Medical Sciences 33, 9–11.Google Scholar
Viteri, F. E. (1996) Weekly compared with daily iron supplementation. American Journal of Clinical Nutrition 63, 610–614.CrossRefGoogle ScholarPubMed
Viteri, F. E., Liu, X., Tolomei, K. & Martin, A. (1995) True absorption and retention of supplemental iron is more efficient when iron is administered every three days rather than daily to iron-normal and iron-deficient rats. Journal of Nutrition 125, 82–91.Google ScholarPubMed
Welch, R. M. & House, W. A. (1980) Absorption of radiocadmium and radioselenium by rats fed intrinsically and extrinsically labelled lettuce leaves. Nutrition Report International 21, 135–145.Google Scholar
Wheby, M. S. & Crosby, W. H. (1963) The gastrointestinal tract and iron absorption. Blood 22, 416–428.Google Scholar
Whittaker, P., Skikne, B. S., Covell, A. M., Flowers, C., Cooke, A., Lynch, S. R. & Cook, J. D. (1989) Duodenal iron proteins in idiopathic haemochromatosis. Journal of Clinical Investigation 83, 261–267.CrossRefGoogle Scholar
Wright, A. J. A. & Southon, S. (1990) The effectiveness of various iron-supplementation regimens in improving the Fe status of anaemic rats. British Journal of Nutrition 63, 579–585.CrossRefGoogle ScholarPubMed
Yip, R. (1994) Iron deficiency: contemporary scientific issues and international programmatic approaches. Journal of Nutrition 124, 1479S–1490S.CrossRefGoogle ScholarPubMed