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The urinary excretion of N-methyl histidine by cattle: validation as an index of muscle protein breakdown

Published online by Cambridge University Press:  09 March 2007

C. I. Harris
Affiliation:
The Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
G. Milne
Affiliation:
The Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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1. The recoveries of radioactivity in cattle urine following the intravenous administration of Nτ-[14CH3]methyl histidine were essentially quantitative in 5–7 d in non-lactating cows, bulls and steers and did not change with age.

2. The Nτ-methyl histidine was excreted unchanged in urine.

3. Nτ-methyl histidine occurred in muscle extracts both in the free form and as a perchloric acid-soluble, acid-labile form which accounted for approximately 85% of the total non-protein-bound Nτ-methyl histidine in muscle and appeared identical to a similar component identified in muscle extracts of sheep and pigs.

4. There was probably an age-related decrease in the concentration of the acid-labile component in muscle but which did not produce a measurable change in recovery of radioactivity in urine.

5. The daily excretion of Nτ-methyl histidine (E, μmol) by male cattle was highly correlated with live weight (W, kg) by the equation: <m:math><m:mrow><m:mtext>E</m:mtext><m:mo>=</m:mo><m:mn>50</m:mn><m:mo>⋅</m:mo><m:mn>4</m:mn><m:mo>+</m:mo><m:mn>3</m:mn><m:mo>⋅</m:mo><m:mn>536</m:mn><m:mo stretchy=“false”>(</m:mo><m:mo>±</m:mo><m:mn>0</m:mn><m:mo>⋅</m:mo><m:mn>044</m:mn><m:mo stretchy=“false”>)</m:mo><m:mtext>W</m:mtext><m:mspace width=“1”/><m:mo stretchy=“false”>(</m:mo><m:mi>r</m:mi><m:mspace width=“0.3”/><m:mn>0</m:mn><m:mo>⋅</m:mo><m:mn>997</m:mn><m:mo stretchy=“false”>)</m:mo><m:mo>.</m:mo></m:mrow></m:math> The excretions progressively decreased from 4.04 μmol/d per kg at 100 kg weight to 3.62 μmol/d per kg at 600 kg.

6. By the criterion of the rate of clearance of labelled Nτ-methyl histidine from the body, the excretion of Nτ-methyl histidine in urine appears to be a valid index of muscle protein breakdown in cattle.

Type
Papers on General Nutrition
Copyright
Copyright © The Nutrition Society 1981

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