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The effect of lucerne-protein concentrate in the diet on growth, reproduction and body composition of Rats

Published online by Cambridge University Press:  07 January 2011

E. L. Hove ,
Evelyn Lohrey ,
M. K. Urs  and
R. M. Allison
E. L. Hove
Affiliation:
Applied Biochemistry Division, DSIR, Palmerston North, New Zealand
Evelyn Lohrey
Affiliation:
Applied Biochemistry Division, DSIR, Palmerston North, New Zealand
M. K. Urs
Affiliation:
Applied Biochemistry Division, DSIR, Palmerston North, New Zealand
R. M. Allison
Affiliation:
Applied Biochemistry Division, DSIR, Palmerston North, New Zealand
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Abstract

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1. Protein concentrates were prepared from freshly cut lucerne by the Pirie process and freeze-dried. When supplemented with methionine or cystine and given to rats as the sole source of protein at 120 g protein/kg diet, the adjusted mean protein efficiency ratio was 2.89 (casein standard at 2.50). As a supplement to protein from barley meal the lucerne leaf-protein concentrate (LPC) was similar to casein.

2. To investigate nutritional safety, lucerne LPC supplemented with methionine was given to rats at high levels for 6 months; exposure of these rats to diffuse daylight was avoided to prevent a severe disfiguring photosensitivity reaction. At a dietary protein concentration of 100 g/kg, rats grew equally well with lucerne LPC or casein. When the supplement was given at protein concentrations of 200 or 300 g/kg the rates of body-weight gain of male and female rats were less than those of control rats given casein. However, after 5 months on the diets, body-weights of male rats had nearly reached those of the controls.

3. Apparent protein digestibility ratio was about 0.80 with all three levels of lucerne LPC.

4. Reproduction was normal in seventeen of the eighteen female rats given the lucerne LPC at the three levels; lactation was also normal and litters were successfully raised to weaning.

5. Organ weights, liver histology and blood haemoglobin were normal in male rats given the lucerne LPC for 6 months.

6. Total body lipid of male rats given lucerne LPC was about half that of the control rats given casein. Body protein was slightly increased, and moisture content was higher in rats given lucerne LPC.

7. The ‘whey’ remaining after precipitation of the protein from lucerne juice strongly inhibited the initial growth of mice given a complete control diet. The mice soon accommodated to the depressive effect of ‘whey’, and body-weight gains were normal during the 3rd week.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 31 , Issue 2 , March 1974 , pp. 147 - 157
Copyright
Copyright © The Nutrition Society 1974

References

REFERENCES

Allison, R. M. (1971). In Leaf Protein, Its Agronomy, Preparation, Quality and Use p. 78 ‘Pirie, N. W., editor]. I.B.P. Handbook no. 20. Oxford: Blackwell Scientific Publications Ltd.Google Scholar
Allison, R. M. (1973). In Chemistry and Biochemistry of Herbage Vol. 3, p. 61 [Bailey, R. W. and Butler, G. W., editors]. London: Academic Press.Google Scholar
Allison, R. M., Laird, W. M. & Synge, R. L. M. (1973). Br. J. Nutr. 29, 51.CrossRefGoogle Scholar
Association of Official Agricultural Chemists (1965). Official Methods of Analysis of the Association of Official Agricultural Chemists. Washington, DC: Association of Official Agricultural Chemists.Google Scholar
Bailey, R. W. (1967). N.Z. Jl agric. Sci. 10, 15.CrossRefGoogle Scholar
Birk, Y. (1969). In Toxic Constituents of Plant Foodstuffs p. 177 [Liener, I. E., editor]. New York: Academic Press Inc.Google Scholar
Byers, M. (1971 a). J. Sci. Fd Agric. 22, 242.CrossRefGoogle Scholar
Byers, M. (1971 b). In Leaf Protein, Its Agronomy, Preparation, Quality and Use p. 95 [Pirie, N. W., editor]. I.B.P. Handbook no. 20. Oxford: Blackwell Scientific Publications Ltd.Google Scholar
Chapman, D. G., Castillo, R. & Campbell, J. A. (1959). Can. J. Biochem. Physiol. 37, 679.CrossRefGoogle Scholar
Chien, F. F. & Mitchell, H. L. (1970). Phytochem. 9, 77.CrossRefGoogle Scholar
Davys, M. N. G. & Pirie, N. W. (1965). J. agric. Engng Res. 8, 70.Google Scholar
Davys, M. N. G. & Pirie, N. W. (1969). Biotech. Bioengng 11, 517.CrossRefGoogle Scholar
Ferrando, R. & Spais, A. (1966). Int. Congr. Nutr. VII. Hamburg p. 276.Google Scholar
Garcha, J. S. & Kawastra, B. L. (1971). J. Fd Sci. Technol. 8, 23.Google Scholar
Hawk, P. B., Oser, B. L. & Summerson, W. H. (1947). Practical Physiological Chemistry p. 616. New York: McGraw-Hill.Google Scholar
Hove, E. L., Fry, G. S. & Schwarz, K. (1958). Proc. Soc. exp. Biol. Med. 98, 27.CrossRefGoogle Scholar
Kohler, G. O. & Bickoff, E. M. (1971). In Leaf Protein, Its Agronomy, Preparation, Quality and Use p. 69 [Pirie, N. W., editor]. I.B.P. Handbook no. 20. Oxford: Blackwell Scientific Publications Ltd.Google Scholar
Lohrey, E., Tapper, B. & Hove, E. L. (1974). Br. J. Nutr. 31, 159.CrossRefGoogle Scholar
Mann, L. T. Jr (1963). Analyt. Chem. 35, 2179.CrossRefGoogle Scholar
Mokady, S. & Zimmermann, G. (1966). Int. Congr. Nutr. VII. Hamburg p. 279.Google Scholar
Morrison, J. E. & Pirie, N. W. (1961). J. Sci. Fd Agric. 12, 1.CrossRefGoogle Scholar
Pick-Seng, Lu & Kinsella, J. E. (1972). J. Fd Sci. 37, 94.Google Scholar
Pirie, N. W. (1971). Leaf Protein, Its Agronomy, Preparation, Quality and Use. I.B.P. Handbook no. 20. Oxford: Blackwell Scientific Publications Ltd.Google Scholar
Subba Rau, B. H., Mahadeviah, S. & Singh, N. (1969). J. Sci. Fd Agric. 20, 355.Google Scholar
Subba Rau, B. H., Ramana, K. V. R. & Singh, N. (1972). J. Sci. Fd. Agric. 23, 233.CrossRefGoogle Scholar
Subba Rau, B. H. & Singh, N. (1971). J. Sci. Fd Agric. 22, 569.CrossRefGoogle Scholar
Woodham, A. A. (1971). In Leaf Protein, Its Agronomy, Preparation, Quality and Use p. 115 [Pirie, N. W., editor]. I.B.P. Handbook no. 20. Oxford: Blackwell Scientific Publications Ltd.Google Scholar