Molecular & Cellular Proteomics
Volume 2, Issue 12, 1 December 2003, Pages 1284-1296
Journal home page for Molecular & Cellular Proteomics

Research
Comprehensive Proteomic Profiling of the Membrane Constituents of a Mycobacterium tuberculosis Strain*S

https://doi.org/10.1074/mcp.M300060-MCP200Get rights and content
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Mycobacterium tuberculosis is an infectious microorganism that causes human tuberculosis. The cell membranes of pathogens are known to be rich in possible diagnostic and therapeutic protein targets. To compliment the M. tuberculosis genome, we have profiled the membrane protein fraction of the M. tuberculosis H37Rv strain using an analytical platform that couples one-dimensional SDS gels to a microcapillary liquid chromatography-nanospray-tandem mass spectrometer. As a result, 739 proteins have been identified by two or more distinct peptide sequences and have been characterized. Interestingly, ∼450 proteins represent novel identifications, 79 of which are membrane proteins and more than 100 of which are membrane-associated proteins. The physicochemical properties of the identified proteins were studied in detail, and then biological functions were obtained by sorting them according to Sanger Institute gene function category. Many membrane proteins were found to be involved in the cell envelope, and those proteins with energy metabolic functions were also identified in this study.

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Published, MCP Papers in Press, October 6, 2003, DOI 10.1074/mcp.M300060-MCP200

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This work was supported by Los Alamos National Laboratory Directed Research Development Grants 20030508ER and 20020048DR and by United States Department of Energy Grants ERW9840 and KP1103010 (to X. C.). This is publication No. LA-UR-03-4877 from the Los Alamos National Laboratory. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

S

The on-line version of this article (available at http://www.mcponline.org) contains Supplementary Tables I and II.