Molecular & Cellular Proteomics
ResearchComparative Proteome Analysis in Schizosaccharomyces pombe Identifies Metabolic Targets to Improve Protein Production and Secretion*
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Author contributions: C.H., T.K., L.C., M.B., K.S., and A.T. designed research; C.H., T.K., L.C., and S.L. performed research; C.H., T.K., L.C., D.L., U.A., M.B., E.H., K.S., and A.T. analyzed data; C.H., T.K., L.C., K.S., and A.T. wrote the paper.
Authors contributions: CWH, TK, SL, LC performed the experiments; CWH, TK, LC, DL, UA, MB, EH, KS, AT interpreted data; CWH, TK, LC, KS and AT wrote the manuscript.
- *
This work was supported by the BMBF (Project: “SweePro- Sustainable and resource-efficient production of sweet proteins for health and nutrition”) and the DFG-Cluster of Excellence “Inflammation at Interfaces,” (Clusterlab CL-X).
This article contains supplemental material.
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The abbreviations used are:
- CDW
cell dry weight
- FDR
false discovery rate
- GIS
global internal standard
- GO
gene ontology
- IP-RP
ion pairing reversed phase
- PPP
pentose phosphate pathway
- TCA
tricarboxylic acid cycle
- μ
growth rate
- 2D-LC
two-dimensional liquid chromatography.
- ‡‡
These authors contributed equally,
- §§
contributed equally as senior authors.